Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

GNAS

Uniprot No.

P63092

Research Area

Signal Transduction

Alternative Names

Adenylate cyclase-stimulating G alpha protein; AHO; alternative gene product encoded by XL-exon; C20orf45; extra large alphas protein; GNAS; GNAS complex locus; GNAS1; GNAS1 gene, formerly; GNAS1, formerly; GNAS2_HUMAN; GPSA; GSA; GSP; guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1; guanine nucleotide regulatory protein; Guanine nucleotide-binding protein G(s) subunit alpha isoforms short; guanine nucleotide-binding protein G(s) subunit alpha isoforms XLas; NESP; neuroendocrine secretory protein; PHP1A; PHP1B; PHP1C; POH; protein ALEX; secretogranin VI

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

1-394aa

Target Protein Sequence

MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKSTIVKQMRILHVNGFNGEGGEEDPQAARSNSDGEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDYILSVMNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

61.7kDa

Protein Length

Full Length

Tag Info

N-terminal 6xHis-SUMO-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The recombinant Human GNAS protein is made through genetic engineering, also called gene splicing or recombinant DNA technology. By putting Human GNAS genes into the genetic material of the E.coli system. These microorganisms can be used as factories or producers to make proteins for medical, academic and research uses. DNA to be manipulated it must be placed within a “transport vehicle” in which proteins may be produced from the genetic code of the DNA. The host cells used for Human GNAS protein synthesis are E.coli cells, the whole production processes include isolation of GNAS gene, amplification of GNAS gene, cloning, GNAS gene selection, and expression, and the GNAS protein purification, the vector contains N-terminal 6xHis-SUMO tag in addition to the specific DNA sequence, this facilitates the purification of the recombinant protein and it’s finally detected with a purity of 90%+ by SDS-PAGE.

GNAS is the encoding gene for the α-subunit of the stimulatory G protein (Gsα), a widely expressed signaling protein that is required for the actions of many hormones and other endogenous molecules by generating cAMP. Mutations in GNAS have been highly linked to McCune-Albright syndrome, characterized by developmental delay, short stature, and skeletal aberrations. GNAS mutation also regulates mucin expression.

Target Background

Function

Guanine nucleotide-binding proteins (G proteins) function as transducers in numerous signaling pathways controlled by G protein-coupled receptors (GPCRs). Signaling involves the activation of adenylyl cyclases, resulting in increased levels of the signaling molecule cAMP. GNAS functions downstream of several GPCRs, including beta-adrenergic receptors. Stimulates the Ras signaling pathway via RAPGEF2.

Involvement in disease

Albright hereditary osteodystrophy (AHO); Pseudohypoparathyroidism 1A (PHP1A); McCune-Albright syndrome (MAS); Progressive osseous heteroplasia (POH); ACTH-independent macronodular adrenal hyperplasia 1 (AIMAH1); Pseudohypoparathyroidism 1B (PHP1B); GNAS hyperfunction (GNASHYP); Pseudohypoparathyroidism 1C (PHP1C)

Subcellular Location

Cell membrane; Lipid-anchor.

Protein Families

G-alpha family, G(s) subfamily

Database Links

HGNC: 4392

OMIM: 103580

KEGG: hsa:2778

UniGene: Hs.125898

Code	CSB-EP352389HU
Abbreviation	Recombinant Human GNAS protein
MSDS	MSDS Datasheet
Size	US$306
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Guanine nucleotide-binding protein G (s) subunit alpha isoforms short (GNAS)

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