Code | CSB-EP887028HU |
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Size | $224 |
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Expressing the recombinant Human IL36A protein in e.coli cells involves inserting a DNA fragment encoding the Human IL36A protein (1-158aa) into a plasmid vector and transferring it to the e.coli cells. Positive cells are screened, cultured, and induced to express the IL36A protein. The protein carries a N-terminal GST tag. The cells are lysed to collect the recombinant Human IL36A protein, which is purified through affinity purification and then identified using SDS-PAGE and subsequent staining of the gel with Coomassie Brilliant Blue. The purity of the resulting recombinant Human IL36A protein exceeds 90%.
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