Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

LPO

Uniprot No.

P22079

Research Area

Others

Alternative Names

LPO; SAPX; Lactoperoxidase; LPO; EC 1.11.1.7; Salivary peroxidase; SPO

Species

Homo sapiens (Human)

Source

Mammalian cell

Expression Region

81-712aa

Target Protein Sequence

TRTAIRNGQVWEESLKRLRQKASLTNVTDPSLDLTSLSLEVGCGAPAPVVRCDPCSPYRTITGDCNNRRKPALGAANRALARWLPAEYEDGLSLPFGWTPGKTRNGFPLPLAREVSNKIVGYLNEEGVLDQNRSLLFMQWGQIVDHDLDFAPDTELGSSEYSKAQCDEYCIQGDNCFPIMFPPNDPKAGTQGKCMPFFRAGFVCPTPPYKSLAREQINALTSFLDASFVYSSEPSLASRLRNLSSPLGLMAVNQEVSDHGLPYLPYDSKKPSPCEFINTTARVPCFLAGDSRASEHILLATSHTLFLREHNRLARELKRLNPQWDGEKLYQEARKILGAFVQIITFRDYLPILLGDHMQKWIPPYQGYSESVDPRISNVFTFAFRFGHLEVPSSMFRLDENYQPWGPEPELPLHTLFFNTWRMVKDGGIDPLVRGLLAKKSKLMKQNKMMTGELRNKLFQPTHRIHGFDLAAINTQRCRDHGQPGYNSWRAFCDLSQPQTLEELNTVLKSKMLAKKLLGLYGTPDNIDIWIGAIAEPLVERGRVGPLLACLLGKQFQQIRDGDRFWWENPGVFTNEQKDSLQKMSFSRLVCDNTRITKVPRDPFWANSYPYDFVDCSAIDKLDLSPWASVKN
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

76.6 kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The region for expressing recombinant Human LPO contains amino acids 81-712. This LPO protein is theoretically predicted to have a molecular weight of 76.6 kDa. Expression of this LPO protein is conducted in mammalian cell. The N-terminal 10xHis tag and C-terminal Myc tag was fused into the coding gene segment of LPO, making it easier to detect and purify the LPO recombinant protein in the later stages of expression and purification.

The human lactoperoxidase (LPO) is an enzyme belonging to the peroxidase family and is primarily found in secretory fluids such as milk, saliva, and tears. LPO plays a crucial role in innate immune defense mechanisms. It contributes to the antimicrobial activity of these fluids by catalyzing the oxidation of thiocyanate and iodide ions, producing reactive intermediates that possess antimicrobial properties. Additionally, LPO is involved in the generation of reactive oxygen species, contributing to the elimination of bacteria and other pathogens. Research on lactoperoxidase focuses on its role in host defense, its potential applications in food preservation, and its relevance to human health.

Target Background

Function

Antimicrobial agent which utilizes hydrogen peroxide and thiocyanate (SCN) to generate the antimicrobial substance hypothiocyanous acid (HOSCN). May contribute to airway host defense against infection.

Gene References into Functions

The crystal structure of the complex of LPO with MZY showed that MZY bound to LPO in the substrate-binding site on the distal heme side. MZY was oriented in the substrate-binding site in such a way that the sulfur atom is at a distance of 2.58 A from the heme iron PMID: 28653416
LPO mRNA was undetectable in the gastric mucosa. PMID: 27048452
Oral peroxidase activity as a marker of chronic alcohol abuse may help in the diagnosis of alcoholism. PMID: 23042278
establish urate as a likely physiological substrate for LPO that will influence host defense and give rise to reactive electrophilic metabolites PMID: 24928513
High intensity of caries is associated with increased levels of some salivary components - sIgA, histatin-5 and lactoperoxidase. PMID: 24974109
a functional LPO system exists in human airways and may contribute to airway host defense against infection PMID: 12626341
lactoperoxidase may play an important role in the metabolic events associated with Parkinson's disease PMID: 15384204
Analysis of the role of thiocyanate (SCN-), in modulating the catalytic activity of myeloperoxidase (MPO) and other members of the lactoperoxidase (LPO) and eosinophil peroxidase (EPO) PMID: 15894800
Splice variants may contribute to LPO molecular heterogeneity and its regulation by intracellular compartmental localization in respiratory epithelium. PMID: 19059195

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Subcellular Location

Secreted.

Protein Families

Peroxidase family, XPO subfamily

Tissue Specificity

Mammary gland; milk and salivary gland. Found in bronchial submucosal glands.

Database Links

HGNC: 6678

OMIM: 150205

KEGG: hsa:4025

STRING: 9606.ENSP00000262290

UniGene: Hs.234742

Code	CSB-MP013066HU
Abbreviation	Recombinant Human LPO protein
MSDS	MSDS Datasheet
Size	$660
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Recombinant Human Lactoperoxidase (LPO)

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