Recombinant Human Nuclear receptor coactivator 1(NCOA1) ,partial

Code CSB-YP015539HU
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Source Yeast
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Code CSB-EP015539HU
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Source E.coli
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Code CSB-EP015539HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP015539HU
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Source Baculovirus
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Code CSB-MP015539HU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names NCOA1
Uniprot No. Q15788
Alternative Names bHLHe74; Class E basic helix-loop-helix protein 74; F SRC 1; Hin 2 protein; Hin2 protein; MGC129719; MGC129720; mNRC 1; NCoA 1; NCoA-1; Ncoa1; NCOA1_HUMAN; Nuclear receptor coactivator 1; Nuclear receptor coactivator protein 1; NY REN 52 antigen; Protein Hin 2; Protein Hin-2; Protein Hin2; Renal carcinoma antigen NY REN 52; Renal carcinoma antigen NY-REN-52; RIP 160; RIP160; SRC 1; SRC-1; Steroid receptor coactivator 1
Species Homo sapiens (Human)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Involved in the coactivation of different nuclear receptors, such as for steroids (PGR, GR and ER), retinoids (RXRs), thyroid hormone (TRs) and prostanoids (PPARs). Also involved in coactivation mediated by STAT3, STAT5A, STAT5B and STAT6 transcription factors. Displays histone acetyltransferase activity toward H3 and H4; the relevance of such activity remains however unclear. Plays a central role in creating multisubunit coactivator complexes that act via remodeling of chromatin, and possibly acts by participating in both chromatin remodeling and recruitment of general transcription factors. Required with NCOA2 to control energy balance between white and brown adipose tissues. Required for mediating steroid hormone response. Isoform 2 has a higher thyroid hormone-dependent transactivation activity than isoform 1 and isoform 3.
Gene References into Functions
  1. Our findings directly associate the AR/NCOA1 complex with PRKD1 regulation and cellular migration and support the concept of therapeutic inhibition of NCOA1 in prostate cancer. PMID: 27255895
  2. SRC-1 can impact osteoblast function in an estrogen receptor-independent manner. PMID: 28286232
  3. The pregnane X receptor down-regulates organic cation transporter 1 (SLC22A1) in human hepatocytes by competing for ("squelching") SRC-1 coactivator. PMID: 26920453
  4. miR-4443 acts in a tumor-suppressive manner by down-regulating TRAF4 and NCOA1 downstream of MEK-C/EBP-mediated leptin and insulin signaling, and that insulin and/or leptin resistance (e.g. in obesity) may suppress this pathway and increase the risk of metastatic CRC. PMID: 27842582
  5. Results provide the first evidence that NCOA1 is a direct target of miR-105-1 suggesting that NCOA1 and miR-105-1 may have potential prognostic value and may be useful as tumor biomarkers for the diagnosis of HCC patients. PMID: 28060733
  6. Our study indicated that genotype and allele frequencies of rs79480871 showed strong associations with MM patients (pa = 3.5x10-4 and pa = 1.5x10-4), and the rs6457327 genotype was more readily associated with MM patients than with controls PMID: 28264017
  7. The mechanism of IL-6-induced AR activation is mediated through enhancing AR-SRC-1 interaction and inhibiting AR-SMRT interaction. PMID: 28396297
  8. IL-6-mediated AR antagonism induced by cypermethrin is related to repress the recruitment of co-regulators SRC-1 and SMRT to the AR in a ligand-independent manner PMID: 27239967
  9. Report novel PAX3-NCOA1 gene fusions in biphenotypic sinonasal sarcomas with focal rhabdomyoblastic differentiation. PMID: 26371783
  10. Data indicate that finerenone inhibits mineralocorticoid receptor (MR), steroid receptor coactivator-1 binding at the regulatory sequence. PMID: 26203193
  11. SRC-1 polymorphisms may be the underlying cause of coronary artery aneurysms in children with Kawasaki disease. PMID: 24652666
  12. Data show that disrupting the steroid receptor coactivator-1 (SRC1) binding site on retinoid X receptor alpha (RXRalpha) alters the transactivation by CAR:RXR. PMID: 25053412
  13. NCOA1 plays a necessary role in E2-induced CXCL12 expression and NCOA2 is required for P4 to inhibit the E2-induced CXCL12 production in normal and ectopic endometrium. PMID: 24586072
  14. SRC1 and Twist1 expression are associated with poor survival in breast cancer. PMID: 24719557
  15. SRC-1 responds to cellular energy stress in tumor cells to promote the expression of genes responsible for oxidative phosphorylation. PMID: 24438340
  16. In a cohort of 453 human breast tumors, NCOA1 and CSF1 levels correlated positively with disease recurrence, higher tumor grade, and poor prognosis. PMID: 24769444
  17. IL-6 secreted by ovarian cancer cells may contribute to the refractoriness of these cells to tamoxifen via the estrogen receptor alpha isoform and SRC-1 (NCOA1). PMID: 24189439
  18. Elucidation of how SRC-1 controls transcription in breast cancers. PMID: 24648347
  19. Overexpression of SRC-1 (NCOA1) and TIF-2 (NCOA2) increases estrogen-induced gene expression. PMID: 12403846
  20. Binding of the ERalpha and ARNT1 AF2 domains to exon 21 of the SRC1 isoform SRC1e is essential for estrogen- and dioxin-transcription. PMID: 22328528
  21. Our data demonstrate that SIP is a novel regulator in caspase-independent and AIF-mediated apoptosis. PMID: 22371500
  22. We have identified a functional genetic variant of SRC-1 with decreased activity, resulting, at least in part, from the loss of a GSK3beta phosphorylation site, which was also associated with decreased bone mineral density in tamoxifen-treated women. PMID: 22174377
  23. findings suggest that SRC-1 switches steroid-responsive tumors to a steroid-resistant state in which the SRC-1 target gene ADAM22 has a critical role PMID: 22072566
  24. The induced AF1 conformation facilitates its interaction with SRC-1, and subsequent AF1-mediated transcriptional activity. PMID: 21760925
  25. NR box-2 and -3 are the essential binding targets for the SRC-1-induced stimulation of LXR transactivity; the competitive in vitro binding of NR box-2 and -3 to LXR was observed. PMID: 20682316
  26. HOXC11 and SRC-1 cooperate to regulate expression of the calcium-binding protein S100beta in resistant breast cancer cells. PMID: 20145129
  27. Studies expand knowledge of PAX3 variant translocations in rhabdomyossarcoma with identification of a novel PAX3-NCOA2 fusion. PMID: 19953635
  28. Clomiphene citrate may inhibit E2-induced endometrial epithelial cell proliferation and ERE transactivation by inhibiting the recruitment of SRC-1 to ER alpha. PMID: 19934375
  29. describes in detail the BRET(2) assay as it is used to examine the physical interaction between the nuclear receptor ERalpha and the transcriptional coactivator SRC-1 PMID: 19763509
  30. CBP/p300 and NcoA/SRC1a may function in a common pathway to regulate STAT3 transcriptional activity. PMID: 11773079
  31. Reduction of coactivator expression by antisense oligodeoxynucleotides inhibits ERalpha transcriptional activity and MCF-7 proliferation PMID: 11818499
  32. CAR antagonizes ER-mediated transcriptional activity by squelching limiting amounts of p160 coactivators, such as SRC-1 and GRIP-1. PMID: 12114525
  33. mutation of the AF2 transactivation domain on Glucocorticoid Receptors disrupts the direct interaction of GR with steroid receptor coactivator 1 (SRC-1) PMID: 12118039
  34. STAT6 has a protein binding motif that controls the interaction with NcoA-1 in transcriptional activation PMID: 12138096
  35. Data show that steroid receptor coactivator-1 (SRC-1) enhanced ligand-independent activation of the AR by IL-6 to the same magnitude as that obtained via ligand-dependent activation, and that activation required MAPK. PMID: 12163482
  36. our results indicate an important role for Mediator, as well as its functional interplay with p300/CBP-SRC, in the enhancement of ERalpha-dependent transcription with chromatin templates PMID: 12482985
  37. Sumoylation was shown to increase progesterone receptor-SRC-1 interaction and to prolong SRC-1 retention in the nucleus. PMID: 12529333
  38. is a coactivator of MHC class II genes that stimulates their interferon gamma (IFNgamma) and class II transactivator (CIITA)-mediated expression. PMID: 12933903
  39. NCoA-1/SRC-1 is an essential coactivator of STAT5 that binds to the FDL motif in the alpha-helical region of the STAT5 transactivation domain PMID: 12954634
  40. differential recruitment of steroid receptor coactivator-1 and silencing mediator for retinoid and thyroid receptors by estrogen receptor-alpha and beta in breast cancer may be central to the response of the tumor to endocrine treatment PMID: 14715875
  41. surface complementarity between the hydrophobic faces of the STAT6 fragment and of the NCoA-1 PAS-B domain almost exclusively defines the binding specificity between the two proteins PMID: 14757047
  42. TNF-alpha impairs progesterone-stimulated PR-B-mediated transactivation, and these effects appear to be due, in part, to reduced expression of SRC-1 and -2 PMID: 15231721
  43. A novel translocation t(2;2)(q35;p23), which generates a fusion protein composed of PAX3 and the NCOA1was identified in rhabdomyosarcoma. PMID: 15313887
  44. Data show that steroid receptor coactivator-1 (SRC-1) overexpressing cells are more responsive to Po mRNA induction by dihydroprogesterone (DHP) than SRC-1-deficient cells, and that DHP treatment increases not only Po but also SRC-1 mRNA levels. PMID: 15456935
  45. The conserved STAT3 region from 752 to 761, called STAT3 CR2, plays critical roles in STAT3-dependent transcription by recruiting SRC-1 and allowing Ser727 phosphorylation. PMID: 15530426
  46. The SRC-1 has been shown to be involved in HIF-1alpha-mediated activation of transcription. PMID: 15615775
  47. SRC-1 and SMRT content change in a tissue-specific manner in the rat brain during the estrous cycle. PMID: 15862975
  48. ASXL1 is a novel coactivator of RAR that cooperates with SRC-1 PMID: 16606617
  49. SRC1-deficient P19 cells showed severely compromised retinoid-induced responses, in agreement with the supposed role of SRC1 as a retinoic acid recepetor coactivator. PMID: 16723356
  50. HPV E7 proteins dysregulate hormone-dependent gene expression by association with and relocalization of SRC-1. PMID: 16775354

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Involvement in disease A chromosomal aberration involving NCOA1 is a cause of rhabdomyosarcoma. Translocation t(2;2)(q35;p23) with PAX3 generates the NCOA1-PAX3 oncogene consisting of the N-terminus part of PAX3 and the C-terminus part of NCOA1. The fusion protein acts as a transcriptional activator. Rhabdomyosarcoma is the most common soft tissue carcinoma in childhood, representing 5-8% of all malignancies in children.
Subcellular Location Nucleus.
Protein Families SRC/p160 nuclear receptor coactivator family
Tissue Specificity Widely expressed.
Database Links

HGNC: 7668

OMIM: 602691

KEGG: hsa:8648

STRING: 9606.ENSP00000320940

UniGene: Hs.596314

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