Recombinant Human Phosphoinositide 3-kinase regulatory subunit 5 (PIK3R5)

Code CSB-EP841225HU
Size $224
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
PIK3R5
Uniprot No.
Research Area
Signal Transduction
Alternative Names
5-bisphosphate 3-kinase regulatory subunit; F730038I15Rik; FOAP 2; FOAP2; OTTHUMP00000135339; p101; P101 PI3K; p101-PI3K; Phosphatidylinositol 4; Phosphatidylinositol 4,5 bisphosphate 3 kinase regulatory subunit; Phosphoinositide 3 kinase, regulatory subunit 5; Phosphoinositide 3 kinase, regulatory subunit 5, p101; Phosphoinositide 3 kinase, regulatory subunit, polypeptide p101; Phosphoinositide 3-kinase regulatory subunit 5; PI3 kinase p101 subunit; PI3 kinase regulatory subunit 5; PI3-kinase p101 subunit; PI3-kinase regulatory subunit 5; PI3R5_HUMAN; PIK3 R5; PIK3R5; Protein FOAP 2; Protein FOAP-2; PtdIns 3 kinase p101; PtdIns 3 kinase regulatory subunit; PtdIns-3-kinase p101; PtdIns-3-kinase regulatory subunit
Species
Homo sapiens (Human)
Source
E.coli
Expression Region
1-494aa
Target Protein Sequence
MDSGYVEDSEESSSEWPWRRGSQERRGHRRPGQKFIRIYKLFKSTSQLVLRRDSRSLEGSSDTALPLRRAGSLCSPLDEPVSPPSRAQRSRSLPQPKLGTQLPSWLLAPASRPQRRRPFLSGDEDPKASTLRVVVFGSDRISGKVARAYSNLRRLENNRPLLTRFFKLQFFYVPVKRSHGTSPGACPPPRSQTPSPPTDSPRHASPGELGTTPWEESTNDISHYLGMLDPWYERNVLGLMHLPPEVLCQQSLKAEAQALEGSPTQLPILADMLLYYCRFAARPVLLQVYQTELTFITGEKTTEIFIHSLELGHSAATRAIKASGPGSKRLGIDGDREAVPLTLQIIYSKGAISGRSRWSNLEKVCTSVNLNKACRKQEELDSSMEALTLNLTEVVKRQNSKSKKGFNQISTSQIKVDKVQIIGSNSCPFAVCLDQDERKILQSVVRCEVSPCYKPEKSDLSSPPQTPPDLPAQAAPDLCSLLCLPIMTFSGALP
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
70.9kDa
Protein Length
Full Length of Isoform 2
Tag Info
N-terminal 6xHis-SUMO-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

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Target Background

Function
Regulatory subunit of the PI3K gamma complex. Required for recruitment of the catalytic subunit to the plasma membrane via interaction with beta-gamma G protein dimers. Required for G protein-mediated activation of PIK3CG.
Gene References into Functions
  1. data argue for differential regulatory functions of the non-catalytic subunits and a specific Gbetagamma-dependent regulation of p101 in PI3Kgamma activation. PMID: 26173259
  2. PIK3R5 promoter hypermethylation is associated with oral squamous cell carcinoma. PMID: 25374236
  3. expression and activities of PI3Kgamma are modified differently by p87 and p101 in vitro and in living cells, arguing for specific regulatory roles of the non-catalytic subunits in the differentiation of PI3Kgamma signaling pathways. PMID: 24014027
  4. Our characterization of the PIK3R5 protein and findings suggest that it may play a role in the development of the cerebellum and vermis. PMID: 22065524
  5. p101 has a role in membrane recruitment and activation of PI3K gamma PMID: 12507995
  6. analysis of functional domains in the p101 regulatory subunit of phosphoinositide 3-kinase gamma PMID: 15611065
  7. Overexpression of p101 activates PI3Kgamma signaling and is associated with T-cell lymphomas PMID: 17486067

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Involvement in disease
Ataxia-oculomotor apraxia 3 (AOA3)
Subcellular Location
Nucleus. Cytoplasm. Cell membrane; Peripheral membrane protein.
Tissue Specificity
Ubiquitously expressed with high expression in fetal brain compared to adult brain. Abundant expression is observed in cerebellum, cerebral cortex, cerebral meninges, and vermis cerebelli.
Database Links

HGNC: 30035

OMIM: 611317

KEGG: hsa:23533

STRING: 9606.ENSP00000392812

UniGene: Hs.278901

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