Recombinant Human Phosphoinositide 3-kinase regulatory subunit 5 (PIK3R5), partial

Code CSB-YP841225HU
MSDS
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Source Yeast
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Code CSB-EP841225HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP841225HU
MSDS
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Source Baculovirus
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Code CSB-MP841225HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PIK3R5
Uniprot No.
Alternative Names
5-bisphosphate 3-kinase regulatory subunit; F730038I15Rik; FOAP 2; FOAP2; OTTHUMP00000135339; p101; P101 PI3K; p101-PI3K; Phosphatidylinositol 4; Phosphatidylinositol 4,5 bisphosphate 3 kinase regulatory subunit; Phosphoinositide 3 kinase, regulatory subunit 5; Phosphoinositide 3 kinase, regulatory subunit 5, p101; Phosphoinositide 3 kinase, regulatory subunit, polypeptide p101; Phosphoinositide 3-kinase regulatory subunit 5; PI3 kinase p101 subunit; PI3 kinase regulatory subunit 5; PI3-kinase p101 subunit; PI3-kinase regulatory subunit 5; PI3R5_HUMAN; PIK3 R5; PIK3R5; Protein FOAP 2; Protein FOAP-2; PtdIns 3 kinase p101; PtdIns 3 kinase regulatory subunit; PtdIns-3-kinase p101; PtdIns-3-kinase regulatory subunit
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Regulatory subunit of the PI3K gamma complex. Required for recruitment of the catalytic subunit to the plasma membrane via interaction with beta-gamma G protein dimers. Required for G protein-mediated activation of PIK3CG.
Gene References into Functions
  1. data argue for differential regulatory functions of the non-catalytic subunits and a specific Gbetagamma-dependent regulation of p101 in PI3Kgamma activation. PMID: 26173259
  2. PIK3R5 promoter hypermethylation is associated with oral squamous cell carcinoma. PMID: 25374236
  3. expression and activities of PI3Kgamma are modified differently by p87 and p101 in vitro and in living cells, arguing for specific regulatory roles of the non-catalytic subunits in the differentiation of PI3Kgamma signaling pathways. PMID: 24014027
  4. Our characterization of the PIK3R5 protein and findings suggest that it may play a role in the development of the cerebellum and vermis. PMID: 22065524
  5. p101 has a role in membrane recruitment and activation of PI3K gamma PMID: 12507995
  6. analysis of functional domains in the p101 regulatory subunit of phosphoinositide 3-kinase gamma PMID: 15611065
  7. Overexpression of p101 activates PI3Kgamma signaling and is associated with T-cell lymphomas PMID: 17486067

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Involvement in disease
Ataxia-oculomotor apraxia 3 (AOA3)
Subcellular Location
Nucleus. Cytoplasm. Cell membrane; Peripheral membrane protein.
Tissue Specificity
Ubiquitously expressed with high expression in fetal brain compared to adult brain. Abundant expression is observed in cerebellum, cerebral cortex, cerebral meninges, and vermis cerebelli.
Database Links

HGNC: 30035

OMIM: 611317

KEGG: hsa:23533

STRING: 9606.ENSP00000392812

UniGene: Hs.278901

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