Recombinant Human Pleckstrin homology domain-containing family F member 2 (PLEKHF2)

Code CSB-YP884492HU
MSDS
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Source Yeast
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Code CSB-EP884492HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP884492HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP884492HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PLEKHF2
Uniprot No.
Alternative Names
110070J07Rik; AA673237; FLJ13187; PH and FYVE domain containing protein 2; PH and FYVE domain-containing protein 2; PH domain containing family F member 2; PH domain-containing family F member 2; Phafin 2; Phafin-2; PHAFIN2; PKHF2_HUMAN; pleckstrin homology domain containing, family F (with FYVE domain) member 2; pleckstrin homology domain containing, family F (with FYVE domain) member2 ; Pleckstrin homology domain-containing family F member 2; Plekhf2; ZFYVE18; Zinc finger FYVE domain containing protein 18 ; Zinc finger FYVE domain-containing protein 18
Species
Homo sapiens (Human)
Expression Region
1-249
Target Protein Sequence
MVDRLANSEA NTRRISIVEN CFGAAGQPLT IPGRVLIGEG VLTKLCRKKP KARQFFLFND ILVYGNIVIQ KKKYNKQHII PLENVTIDSI KDEGDLRNGW LIKTPTKSFA VYAATATEKS EWMNHINKCV TDLLSKSGKT PSNEHAAVWV PDSEATVCMR CQKAKFTPVN RRHHCRKCGF VVCGPCSEKR FLLPSQSSKP VRICDFCYDL LSAGDMATCQ PARSDSYSQS LKSPLNDMSD DDDDDDSSD
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
May play a role in early endosome fusion upstream of RAB5, hence regulating receptor trafficking and fluid-phase transport. Enhances cellular sensitivity to TNF-induced apoptosis.
Gene References into Functions
  1. PtdIns(3)P binding to Phafin2 occurs with high affinity, triggering minor conformational changes in the protein. Taken together, these studies represent a platform for establishing the structural basis of Phafin2 molecular interactions and the role of the two potentially redundant PtdIns(3)P-binding domains of the protein in endomembrane compartments. PMID: 28152563
  2. These findings establish that lysosomal accumulation of Akt and Phafin2 is a critical step in the induction of autophagy via an interaction with phosphatidylinositol 3 phosphate. PMID: 24416124
  3. Phafin2 controls EGFR trafficking through early endosomes by facilitating endosome fusion in concert with EEA1. PMID: 22816767
  4. These results provide a vivid example that an endosome modulator, such as Phafin2, may control the cells' responses to the extracellular cues. PMID: 19995552
  5. Results demonstrate that EAPF/Phafin-2 facilitates TNF-alpha-induced cellular apoptosis through an ER-mitochondrial apoptotic pathway. PMID: 18288467

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Subcellular Location
Early endosome membrane; Peripheral membrane protein. Endoplasmic reticulum.
Tissue Specificity
Expressed in placenta, ovary and small intestine, as well as in heart and pancreas. Also expressed in peripheral blood mononuclear cells and dendritic cells.
Database Links

HGNC: 20757

OMIM: 615208

KEGG: hsa:79666

STRING: 9606.ENSP00000322373

UniGene: Hs.29724

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