Recombinant Human Pleckstrin homology domain-containing family F member 2 (PLEKHF2)

Code CSB-EP884492HU
Size $224
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
PLEKHF2
Uniprot No.
Research Area
Cell Biology
Alternative Names
110070J07Rik; AA673237; FLJ13187; PH and FYVE domain containing protein 2; PH and FYVE domain-containing protein 2; PH domain containing family F member 2; PH domain-containing family F member 2; Phafin 2; Phafin-2; PHAFIN2; PKHF2_HUMAN; pleckstrin homology domain containing, family F (with FYVE domain) member 2; pleckstrin homology domain containing, family F (with FYVE domain) member2 ; Pleckstrin homology domain-containing family F member 2; Plekhf2; ZFYVE18; Zinc finger FYVE domain containing protein 18 ; Zinc finger FYVE domain-containing protein 18
Species
Homo sapiens (Human)
Source
E.coli
Expression Region
1-249aa
Target Protein Sequence
MVDRLANSEANTRRISIVENCFGAAGQPLTIPGRVLIGEGVLTKLCRKKPKARQFFLFNDILVYGNIVIQKKKYNKQHIIPLENVTIDSIKDEGDLRNGWLIKTPTKSFAVYAATATEKSEWMNHINKCVTDLLSKSGKTPSNEHAAVWVPDSEATVCMRCQKAKFTPVNRRHHCRKCGFVVCGPCSEKRFLLPSQSSKPVRICDFCYDLLSAGDMATCQPARSDSYSQSLKSPLNDMSDDDDDDDSSD
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
54.8kDa
Protein Length
Full Length
Tag Info
N-terminal GST-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

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Target Background

Function
May play a role in early endosome fusion upstream of RAB5, hence regulating receptor trafficking and fluid-phase transport. Enhances cellular sensitivity to TNF-induced apoptosis.
Gene References into Functions
  1. PtdIns(3)P binding to Phafin2 occurs with high affinity, triggering minor conformational changes in the protein. Taken together, these studies represent a platform for establishing the structural basis of Phafin2 molecular interactions and the role of the two potentially redundant PtdIns(3)P-binding domains of the protein in endomembrane compartments. PMID: 28152563
  2. These findings establish that lysosomal accumulation of Akt and Phafin2 is a critical step in the induction of autophagy via an interaction with phosphatidylinositol 3 phosphate. PMID: 24416124
  3. Phafin2 controls EGFR trafficking through early endosomes by facilitating endosome fusion in concert with EEA1. PMID: 22816767
  4. These results provide a vivid example that an endosome modulator, such as Phafin2, may control the cells' responses to the extracellular cues. PMID: 19995552
  5. Results demonstrate that EAPF/Phafin-2 facilitates TNF-alpha-induced cellular apoptosis through an ER-mitochondrial apoptotic pathway. PMID: 18288467

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Subcellular Location
Early endosome membrane; Peripheral membrane protein. Endoplasmic reticulum.
Tissue Specificity
Expressed in placenta, ovary and small intestine, as well as in heart and pancreas. Also expressed in peripheral blood mononuclear cells and dendritic cells.
Database Links

HGNC: 20757

OMIM: 615208

KEGG: hsa:79666

STRING: 9606.ENSP00000322373

UniGene: Hs.29724

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