Recombinant Human Pregnancy-specific beta-1-glycoprotein 1 (PSG1)

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Code CSB-EP018849HU
Size $256
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 85% as determined by SDS-PAGE.
Target Names
PSG1
Uniprot No.
Research Area
Tags & Cell Markers
Alternative Names
(PS-beta-G-1)(PSBG-1)(Pregnancy-specific glycoprotein 1)(CD66 antigen-like family member F)(Fetal liver non-specific cross-reactive antigen 1/2)(FL-NCA-1/2)(PSG95)(Pregnancy-specific beta-1 glycoprotein C/D)(PS-beta-C/D)(CD antigen CD66f)
Species
Homo sapiens (Human)
Source
E.coli
Expression Region
35-419aa
Target Protein Sequence
QVTIEAEPTKVSEGKDVLLLVHNLPQNLTGYIWYKGQMRDLYHYITSYVVDGEIIIYGPAYSGRETAYSNASLLIQNVTREDAGSYTLHIIKGDDGTRGVTGRFTFTLHLETPKPSISSSNLNPRETMEAVSLTCDPETPDASYLWWMNGQSLPMTHSLKLSETNRTLFLLGVTKYTAGPYECEIRNPVSASRSDPVTLNLLPKLPKPYITINNLNPRENKDVLNFTCEPKSENYTYIWWLNGQSLPVSPRVKRPIENRILILPSVTRNETGPYQCEIRDRYGGIRSDPVTLNVLYGPDLPRIYPSFTYYRSGEVLYLSCSADSNPPAQYSWTINEKFQLPGQKLFIRHITTKHSGLYVCSVRNSATGKESSKSMTVEVSDWTVP
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
49.5 kDa
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description

The production of recombinant Human PSG1 protein involves manipulating the expression of the desired gene in e.coli cells. The sequence (35-419aa) of foreign DNA is merged with an expression vector and subsequently introduced into e.coli cells. Positive cells are chosen and cultivated to induce the expression of the desired protein. A N-terminal 6xHis tag is fused to the protein. The resulting recombinant Human PSG1 protein undergoes purification through affinity purification, with a purity exceeding 85%, as confirmed by SDS-PAGE.

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Target Background

Gene References into Functions
  1. Inhibiting PSG1 can effectively reduce chemoresistance via a transforming growth factor-beta (TGF-beta)-related pathway in ER-negative breast cancer cells. PMID: 27653744
  2. PSG stimulated IDO activity under the conditions of induction of the monocytes by interferon-gamma. PMID: 27595833
  3. Under conditions of directed induction of phenotypic changes in CD4(+) lymphocytes to regulatory T cells (Treg), pregnancy-specific at high concentrations (10 and 100 mug/mL) increased FOXP3 expression. The evaluation of the spontaneous expression level of FOXP3 mRNA showed that pregnancy-specific beta1-glycoprotein (1 and 100 mug/mL) stimulated the expression of this factor in mononuclear cells and isolated CD4(+) lym... PMID: 27817017
  4. results identify a non-placental site of PSG expression in the gut and associated tumors, with implications for determining whether PSGs have a role in tumor progression, and utility as tumor biomarkers. PMID: 26926266
  5. the presence of GAGs on the surface of endothelial cells was required for the ability of PSG1 to induce tube formation PMID: 21193412
  6. The aspartic acid at position 95 is not required for PSG1 activity but the amino acids implicated in the formation of a salt bridge within the N-domain are essential. PMID: 20335639
  7. first trimester maternal serum marker of small-for-gestational age and preterm delivery PMID: 19911417
  8. Human PSG1a induces alternative activation in human & mouse monocytes & suppresses the accessory-cell-dependent T-cell proliferation. PSG1a contributes to immunomodulation during pregnancy, with opposite effects on maternal innate & adaptative systems. PMID: 12223519
  9. The differential expression of HLA-E and PSG1 in the sperm of fertile and infertile men is discussed. PMID: 18987160

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Subcellular Location
Secreted.
Protein Families
Immunoglobulin superfamily, CEA family
Database Links

HGNC: 9514

OMIM: 176390

KEGG: hsa:5669

STRING: 9606.ENSP00000244296

UniGene: Hs.709192

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