Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

PDCD2

Uniprot No.

Q16342

Research Area

Cell Biology

Alternative Names

Zinc finger MYND domain-containing protein 7 Zinc finger protein Rp-8

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

1–344aa

Target Protein Sequence

MAAAGARPVELGFAESAPAWRLRSEQFPSKVGGRPAWLGAAGLPGPQALACELCGRPLSFLLQVYAPLPGRPDAFHRCIFLFCCREQPCCAGLRVFRNQLPRKNDFYSYEPPSENPPPETGESVCLQLKSGAHLCRVCGCLGPKTCSRCHKAYYCSKEHQTLDWRLGHKQACAQPDHLDHIIPDHNFLFPEFEIVIETEDEIMPEVVEKEDYSEIIGSMGEALEEELDSMAKHESREDKIFQKFKTQIALEPEQILRYGRGIAPIWISGENIPQEKDIPDCPCGAKRILEFQVMPQLLNYLKADRLGKSIDWGILAVFTCAESCSLGTGYTEEFVWKQDVTDTP
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

44.6 kDa

Protein Length

Full Length

Tag Info

N-terminal 10xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

To achieve the expression of the recombinant Human PDCD2 protein in e.coli cells, a DNA fragment encoding the Human PDCD2 protein (1–344aa) is inserted into a plasmid vector, which is then transferred to e.coli cells. After screening positive cells, they are cultured and induced to creat the PDCD2 protein. The protein features a N-terminal 10xHis tag fusion. The recombinant Human PDCD2 protein is purified from the cell lysate through affinity purification. The purity of the obtained recombinant Human PDCD2 protein is greater than 85%, measured by SDS-PAGE.

Target Background

Function

May be a DNA-binding protein with a regulatory function. May play an important role in cell death and/or in regulation of cell proliferation.

Gene References into Functions

PDCD2 and NCoR1 may act as tumor suppressors in Gastrointestinal stromal tumors cells through the Smad signaling pathway. PMID: 26589942
PDCD2 may play an important role in tumor suppression in osteosarcoma. These mechanisms might be related to immune response induced by CD4(+) and CD8(+) T cells. PMID: 26617804
Low PDCD2 expression is associated with gastric cancer. PMID: 25111461
Loss of PDCD2 expression could contribute to gastric cancer development and progression. PMID: 25334010
Expression of human PDCD2 fully rescues the Drosophila Zfrp8 phenotype, underlining the functional conservation of Zfrp8/PDCD2. PMID: 24381196
PDCD2 RNA expression in acute leukemia patients correlates with disease status and is a significant predictor of clinical relapse. PMID: 23760497
PDCD2 has a novel regulatory role in human hematopoiesis and is essential for erythroid development. PMID: 22922207
Transfection of a construct expressing PDCD2 induces apoptosis in human cell lines through activation of the caspase cascade. PMID: 20605493
To study the role of PDCD2_C domain in apoptosis, the cDNAs of two isoforms of PDCD2 and MGC13096 were cloned. PDCD2 (NM_002598) was over expressed when endothelial cells treated with leukotriene D4 or natural killer cells were activated by IL-2. PMID: 16311922
repression of PDCD2 by BCL6 is likely important in the pathogenesis of certain human lymphomas PMID: 17468402

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Subcellular Location

Nucleus.

Tissue Specificity

Ubiquitous.

Database Links

HGNC: 8762

OMIM: 600866

KEGG: hsa:5134

STRING: 9606.ENSP00000439467

UniGene: Hs.367900

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Code	CSB-EP618070HU
Abbreviation	Recombinant Human PDCD2 protein
MSDS	MSDS Datasheet
Size	$306
	Order now
Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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