Greater than 85% as determined by SDS-PAGE.
hWNT 5A; hWNT5A; Protein Wnt 5a; Protein Wnt-5a; Protein Wnt5a; Wingless type MMTV integration site family member 5A; Wnt 5a; WNT 5A protein; WNT 5A protein precursor; WNT5A; WNT5A protein precursor; WNT5A_HUMAN
Homo sapiens (Human)
Target Protein Sequence
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Full Length of Mature Protein
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
3-7 business days
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
The expression of recombinant human WNT5A protein includes the construction of the expression vector containing the recombinant DNA and the transformation of the expression vector into the E.coli, which provides a variety of macromolecules and components required for transcription and translation. The recombinant DNA was formed by fusing the N-terminal 6xHis tag sequence to the designated sequence encoding the 62-380aa of the human WNT5A protein. This N-terminal GST-tagged recombinant human WNT5A protein is also characterized by high purity, >85%. Under SDS-PAGE condition, this recombinant WNT5A protein migrated to the band of about 40 kDa molecular weight. It may have applications in WNT5A-mediated cancer research.
WNT5B is considered to be a non-classical Wnt protein in the process of fat differentiation. Wnt protein family includes at least 19 members, which is a group of secretory glycoproteins rich in cysteine and participates in many biological processes such as embryonic development, cell proliferation and differentiation, and even tumor occurrence. Wnt5b is a member of the same family of proteins as Wnt5a. Evidence has accumulated that Wnt5b expression is involved in mouse chondrocyte maturation. Wnt5b were reported to be highly expressed in head and neck squamous cell carcinomas. Other studies also found highly expressed in breast cancer with high invasive activity. Of late, the role of Wnt5b overexpression in cancer aggressiveness has been identified. For example, in lung cancer and pancreatic cancer cells, upregulated Wnt5b has been observed, but the biochemical properties and cancer-associated activities of Wnt5b have not been well characterized.