Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

PPP1R1B

Uniprot No.

Q9UD71

Research Area

Neuroscience

Alternative Names

(DARPP-32)(Dopamine- and cAMP-regulated neuronal phosphoprotein)

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

1-168aa

Target Protein Sequence

MDPKDRKKIQFSVPAPPSQLDPRQVEMIRRRRPTPAMLFRLSEHSSPEEEASPHQRASGEGHHLKSKRPNPCAYTPPSLKAVQRIAESHLQSISNLNENQASEEEDELGELRELGYPREEDEEEEEDDEEEEEEEDSQAEVLKVIRQSAGQKTTCGQGLEGPWERPPP
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

23.2 kDa

Protein Length

Full Length of Isoform 2

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

An appropriate expression vector successfully incorporates the full-length human PPP1R1B gene fragment, spanning amino acids 1-168 and featuring an N-terminal 6xHis-tag. Subsequently, the recombinant expression vector harboring the PPP1R1B gene fragment is introduced into competent E. coli cells. Following a meticulous selection process, the transformed cells are cultured to enable the expression of the desired recombinant protein. After a suitable induction period, the E. coli cells are harvested and lysed, resulting in the release of cellular contents, including the expressed recombinant PPP1R1B protein. SDS-PAGE analysis reveals the purity of the obtained human PPP1R1B protein to surpass 90%.

Target Background

Function

Inhibitor of protein-phosphatase 1.

Gene References into Functions

t-Darpp phosphorylation at T39 seems to be crucial for t-Darpp-mediated PKA activation and this activation appears to occur through an association with RI and sequestering of RI away from PKAc. The t-Darpp-RI interaction could be a druggable target to reduce PKA activity in drug-resistant cancer. PMID: 28867659
our results lend support for a potential role of DARPP-32 genetic variants in neural response to potential reward triggering cues PMID: 29048604
To facilitate live-cell identification, a human embryonic stem cell line was generated using CRISPR-mediated gene editing at the DARPP32 locus. PMID: 28065797
Study associates polymorphisms in DRD2, DARPP-32, and COMT genes with novel category learning performance. Modeling results suggest that striatal dopaminergic genes influence selective attention processes whereas cortical genes mediate the ability to update complex rule representations. PMID: 26918585
Results show that expression of human DARPP-32 protein isoforms depends on the striatal neurodevelopmental stage with t-DARPP being specific for the human adult striatum. PMID: 27475250
In vivo xenograft models demonstrated that overexpression of DARPP-32 promotes angiogenesis and tumour growth. PMID: 25779598
This study strongly suggests that inflammation-regulated DARPP-32 constitutes a key component of H. pylori-mediated gastric tumourigenesis. PMID: 27590997
This study did not find any statistical difference in the state-anxiety dimension for the rs12601930C/T, rs879606A/G, and rs3764352A/G genotypes, but statistical differences in the trait-anxiety dimension for the rs879606A/G (F = 5.207, P = 0.006) and rs3764352A/G (F = 3.960, P = 0.020) genotype. PMID: 27995567
DARPP-32 is mainly cleaved at Thr(153) by calpain and this cleavage of DARPP-32 reduces CREB phosphorylation via loss of its inhibitory function on PP1; results suggest a novel mechanism of DARPP-32-CREB signalling dysregulation in Alzheimer's disease PMID: 26178297
No significant differences in mRNA expression levels of DRD2 and DARPP-32 were found among controls, patients with psychotic disorder not otherwise specified, and schizophrenia/schizophreniform disorder. PMID: 26561806
Striatal function was indexed by a gene coding for DARPP-32, which is densely expressed in the striatum where it is necessary for synaptic plasticity PMID: 26818509
Findings show that PPP1R1B-STARD3 fusion transcript has a key role in subsets of gastric cancers through the activation of PI3K/AKT signaling. PMID: 24276243
The results of this study found that the mean t-DARPP expression level in the caudate was higher in patients with schizophrenia than in control individuals. PMID: 24704945
Results suggest that variation in PPP1R1B affects the abundance of the splice variant t-DARPP-32 mRNA and may reflect potential molecular mechanisms implicated in schizophrenia and affective disorders PMID: 23295814
The immunostaining was more decreased for DARPP-32 than for the morphological changes of striatal neurons in multiple system atrophy. PMID: 23715974
This study showed that variations in genotypes relevant for DARPP-32 functions are associated more with aging-related impairments in the explicit than the implicit component of sequence learning. PMID: 24035787
density of DARPP-32-immunoreactive (IR) neurons in the II and III layers of the RAIC was significantly decreased (p<0.05) in the schizophrenia group compared with the healthy control group. PMID: 21821092
Bcl-2 and DARPP-32 have roles in regulating inositol 1,4,5-trisphosphate receptor phosphorylation and promoting cell survival PMID: 24395794
High DARPP32/STAT3, DARPP32/STAT5B, and STAT5B/STAT3 ratios were associated with longer patient progression free survival. PMID: 23250732
The present study provides novel evidence for an association between the DARPP-32 gene (SNP rs907094) and attentional control of auditory perception. PMID: 23639477
Findings show a novel mechanism by which DARPP-32 promotes cell invasion by regulating CXCR4-mediated activation of the MT1-MMP/MMP-2 pathway. PMID: 23160836
PPP1R1B rs12601930 was associated with projection and splitting. rs3764352 was associated with splitting. No significant association was found between rs879606 and defenses. PPP1R1B polymorphisms are involved in immature defenses. PMID: 23080070
Our findings support a role for the DRD2 and PPP1R1B genes in conferring risk for autism in families with only affected males and show an additive effect of these genes towards prediction of affected status in our families. PMID: 22559203
Our findings uncover a novel mechanism of TRAIL resistance mediated by DARPP-32, whereby it inhibits the intrinsic apoptosis pathway through upregulation of BCL-xL PMID: 22589394
DARPP32 has a role in pluripotent stem cell differentiation toward medium-sized spiny neurons PMID: 23250204
no significant association of BDNF or DARPP-32 polymorphisms with schizophrenia in Malays. PMID: 22576830
the decrease in DARPP-32 in schizophrenia was more marked in neurons of dorsolateral prefrontal cortex than in other cells or other brain regions PMID: 22179181
DARPP-32 increases interactions between epidermal growth factor receptor and ERBB3 to promote tumor resistance to gefitinib. PMID: 21741919
[review] Genetic polymorphisms modulating DARPP-32 mRNA expression and cognition in humans are associated with changes in activation in the entire striatum, and striatal connectivity with frontal cortex, without connection to any other brain region. PMID: 20631684
the DARPP-32-related pathogenesis in schizophrenia may be more severe in the superior temporal gyrus than previously found in the prefrontal cortex PMID: 21453742
Results suggest that polymorphisms in the DARPP-32 gene are involved in the biological mechanisms that confer the traits of novelty seeking and harm avoidance. PMID: 21369787
Findings demonstrate that t-DARPP regulates beta-catenin/TCF activity, thereby implicating a novel oncogenic signaling in upper gastrointestinal cancers. PMID: 21447180
our data suggest: (i) C14ORF28, GNB2L1, MLLT3, DRD2 and DARPP-32 are important in the pathogenesis of schizophrenia and bipolar disorder PMID: 20874815
Findings underscore the potential role of t-DARPP in regulating cell growth and proliferation through PI3 kinase-dependent mechanism. PMID: 20836878
This study did not find DAOA significant associations with schizophrenia. Thus, PPP1R1B genes do not fit the antagonistic pleiotropy PMID: 20483474
The composite expression score, calculated from immunostaining patterns, increased significantly from normal or gastritis to metaplasia, dysplasia, and adenocarcinoma (P < .001). PMID: 20580047
DARPP-32 changes the usage of tra2-beta1 dependent alternative exons in a concentration-dependent manner, suggesting that the DARPP-32:tra2-beta1 interaction is a molecular link between signaling pathways and pre-mRNA processing. PMID: 20074680
t-Darpp and DARPP-32 expression are novel prognostic and predictive biomarkers in breast cancer. PMID: 19301121
DARPP 32 frequently are overexpressed in common subtypes of human adenocarcinomas suggest that these proteins may be important in tumorigenesis. PMID: 14508844
overexpression of DARPP-32 is associated with gastric cancer PMID: 14991576
DARPP-32 expression arises after a phase of dysplasia esophageal squamous cell carcinoma; tumours expressing DARPP-32 progress less rapidly than DARPP-32-negative tumours PMID: 15188007
multiple domains in I-1 target cellular PP1 complexes, and I-1 has a role as a cellular regulator of eIF2alpha phosphorylation PMID: 15345721
Expression of Darpp-32 is associated with a potent antiapoptotic advantage for cancer cells through a p53-independent mechanism that involves preservation of mitochondrial potential and increased Bcl2 levels. PMID: 16061638
Thus, DARPP-32 signaling downstream of DDR1 is a potential new target for effective anti-metastatic breast cancer therapy PMID: 17027969
Potential involvement of PPP1R1B in the etiology of nicotine dependence. PMID: 17171661
DARPP-32 plays a pivotal role in cognitive function and possibly in the pathogenesis of schizophrenia. PMID: 17290303
The up-regulation of ZNRD1 significantly inhibited the drug sensitivity of gastric cancer cells over-expressing DARPP-32, indicating that ZNRD1 may be important in the DARPP-32-mediated MDR of gastric cancer PMID: 17492506
The present study suggests that DARPP-32 decreases in the DLPFC of patients with schizophrenia and bipolar disorder. PMID: 17521792
The results of this study do not preclude the possibility that the PPP1R1B is a susceptibility gene for schizophrenia in the Chinese population. PMID: 17618027
The decreased expression of DARPP-32 in oral premalignant and malignant lesions suggests a tumor suppressor role for this protein in the tumorigenesis of these lesions. PMID: 17695523

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Subcellular Location

Cytoplasm.

Protein Families

Protein phosphatase inhibitor 1 family

Database Links

HGNC: 9287

OMIM: 604399

KEGG: hsa:84152

STRING: 9606.ENSP00000254079

UniGene: Hs.286192

Code	CSB-EP880070HUa0
Abbreviation	Recombinant Human PPP1R1B protein
MSDS	MSDS Datasheet
Size	$306
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Protein phosphatase 1 regulatory subunit 1B (PPP1R1B)

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