Recombinant Human Rho guanine nucleotide exchange factor 1(ARHGEF1) ,partial

Code CSB-YP856434HU
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Source Yeast
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Code CSB-EP856434HU
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Source E.coli
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Code CSB-EP856434HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP856434HU
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Source Baculovirus
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Code CSB-MP856434HU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names ARHGEF1
Uniprot No. Q92888
Alternative Names 115 kD protein; 115 kDa guanine nucleotide exchange factor; ARHG1_HUMAN; ARHGEF 1; ARHGEF1; GEF 1; GEF1; LBCL 2; LBCL2; LSC; Lsc homolog; p115 RhoGEF; p115-RhoGEF; p115RhoGEF; Rho guanine nucleotide exchange factor (GEF) 1; Rho guanine nucleotide exchange factor 1; Sub1.5
Species Homo sapiens (Human)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Data

Function Seems to play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits. Acts as GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF) for RhoA GTPase. Activated G alpha 13/GNA13 stimulates the RhoGEF activity through interaction with the RGS-like domain. This GEF activity is inhibited by binding to activated GNA12. Mediates angiotensin-2-induced RhoA activation.
Gene References into Functions
  1. Data suggest that MCP1/CCL2 induces activation/tyrosine phosphorylation of ARHGEF1/p115-RhoGEF and up-regulates RAC1 signaling in vascular smooth muscle cells (VSMCs); ARHGEF1 inhibition suppresses MCP1-induced VSMC migration and proliferation. (ARHGEF1 = Rho guanine nucleotide exchange factor 1; RAC1 = Rac family small GTPase 1; CCL2 = C-C motif chemokine ligand 2) PMID: 28655771
  2. Data indicate that the crystal structure of PDZ-RhoGEF PDZ domain in complex with the CXC chemokine receptor 2 (CXCR2) C-terminal PDZ binding motif. PMID: 28179147
  3. We have reported here for the first time a reduced activity of both Rac1 and Cdc42 in human pheochromocytoma resection as well as tumor-associated expression changes of FARP1, ARHGEF1, and ARHGAP36 PMID: 26911374
  4. contactin-1 displayed the ability to phosphorylate the RhoA activator p115 RhoGEF PMID: 25916117
  5. The novel role for p115RhoGEF in regulation of epithelial plasticity is dependent on Rho-DRF signaling module. PMID: 24465552
  6. Regulated localization is sufficient for hormonal control of regulator of G protein signaling homology Rho guanine nucleotide exchange factors (RH-RhoGEFs). PMID: 24855647
  7. Modification of p115RhoGEF at Serine(330) regulates its RhoGEF activity. PMID: 23816534
  8. The action of DOCK7 in vivo may involve the coordinated integration of Cdc42/Rac1 signaling in the context of the membrane recruitment of a DOCK7 guanine nucleotide exchange factor (GEF) complex. PMID: 23718289
  9. High GEF1 expression is associated with metastasis of pancreatic cancer. PMID: 23070684
  10. Activation of p115-RhoGEF requires direct association of Galpha13 and the Dbl homology domain. PMID: 22661716
  11. Upon beta(2)AR activation, both betaArrestin2 and p115RhoGEF translocate to the plasma membrane, with concomitant activation of RhoA and formation of focal adhesions and stress fibers. PMID: 22500016
  12. Thromboxane receptor signaling is required for fibronectin-induced matrix metalloproteinase 9 production by human and murine macrophages and is attenuated by the Arhgef1 molecule. PMID: 22086927
  13. Mechanistic insights into specificity, activity, and regulatory elements of the regulator of G-protein signaling (RGS)-containing Rho-specific guanine nucleotide exchange factors (GEFs) p115, PDZ-RhoGEF (PRG), and leukemia-associated RhoGEF (LARG). PMID: 21454492
  14. The linker region connecting the N-terminal RGS-homology domain and the Dbl homology domain inhibits the intrinsic guanine nucleotide exchange activity of p115. PMID: 21064165
  15. ARHGEF1 is involed in hypertension by controlling its molecular mechanisms. PMID: 20619149
  16. coexpression of a dominant negative PDZ-RhoGEF abrogated the ability of plexin-B1 to cause stress fiber formation PMID: 12372594
  17. rgRGS domain may serve a structural or allosteric role in the regulation of the nucleotide exchange activity of p115RhoGEF on Rho by Galpha(13) PMID: 12525488
  18. CD44 interaction with p115RhoGEF and ROK plays a pivotal role in promoting Gab-1 phosphorylation leading to Gab-1.PI 3-kinase membrane localization, AKT signaling, and cytokine (M-CSF) production during HA-mediated breast cancer progression PMID: 12748184
  19. data demonstrate a pathway of Rho activation involving protein kinase c alpha-dependent phosphorylation of p115Rho guanine exchange factor PMID: 12754211
  20. Data show that different rho guanine nucleotide exchange factors (rhoGEFs; p115rhoGEF, LARG and PDZrhoGEF) mediate downstream rho signaling by the thrombin and lysophosphatidic acid receptors. PMID: 15143072
  21. Several features of a typical alpha/RGS interaction are preserved in the alpha(13)/p115RhoGEF interaction. PMID: 15735747
  22. analysis of a novel cross-talk exerted from the LPA/Galpha(13)/p115RhoGEF/RhoA pathway to the beta(2)-adrenergic receptor/Galpha(s)/adenylyl cyclase pathway PMID: 17493936
  23. A pronounced and rapid translocation of p115-RhoGEF from the cytosol to the plasma membrane was observed upon activation of several G(12/13)-coupled receptors in a cell type-independent fashion. PMID: 18320579
  24. Data show that microtubules in neighboring cells reorient and target p115 RhoGEF to sites where dying cells are squeezed out of the epithelial sheet. PMID: 19720875

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Subcellular Location Cytoplasm, Membrane
Tissue Specificity Ubiquitously expressed.
Database Links

HGNC: 681

OMIM: 601855

KEGG: hsa:9138

STRING: 9606.ENSP00000337261

UniGene: Hs.631550

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