Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

SRR

Uniprot No.

Q9GZT4

Research Area

Neuroscience

Alternative Names

D serine ammonia lyase; D serine dehydratase; D-serine ammonia-lyase; D-serine dehydratase; ILV1; ISO1; L serine ammonia lyase; L serine dehydratase; L-serine ammonia-lyase; L-serine dehydratase; Serine racemase; srr; SRR_HUMAN

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

1-340aa

Target Protein Sequence

MCAQYCISFADVEKAHINIRDSIHLTPVLTSSILNQLTGRNLFFKCELFQKTGSFKIRGALNAVRSLVPDALERKPKAVVTHSSGNHGQALTYAAKLEGIPAYIVVPQTAPDCKKLAIQAYGASIVYCEPSDESRENVAKRVTEETEGIMVHPNQEPAVIAGQGTIALEVLNQVPLVDALVVPVGGGGMLAGIAITVKALKPSVKVYAAEPSNADDCYQSKLKGKLMPNLYPPETIADGVKSSIGLNTWPIIRDLVDDIFTVTEDEIKCATQLVWERMKLLIEPTAGVGVAAVLSQHFQTVSPEVKNICIVLSGGNVDLTSSITWVKQAERPASYQSVSV
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

52.6kDa

Protein Length

Full Length

Tag Info

N-terminal 6xHis-SUMO-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The generation of the recombinant Human SRR protein typically includes the following steps: synthesizing the recombinant plasmid containing the gene encoding the Human SRR protein (1-340aa), transforming the recombinant plasmid into e.coli cells, selecting the positive e.coli cells, and culturing the positive e.coli cells for protein expression. The protein is equipped with a N-terminal 6xHis-SUMO tag. After expression, affinity purification is employed to isolate and purify the recombinant SRR protein from the cell lysate. Denaturing SDS-PAGE is utilized to resolve the resulting recombinant Human SRR protein, revealing a purity level exceeding 90%.

Target Background

Function

Catalyzes the synthesis of D-serine from L-serine. D-serine is a key coagonist with glutamate at NMDA receptors. Has dehydratase activity towards both L-serine and D-serine.

Gene References into Functions

SRR was identified as a type 2 diabetes susceptibility gene. SRR plays a role in insulin secretion in vitro. PMID: 28580277
rs391300 SNP, located on the serine racemase (SRR) gene and linked to increased susceptibility to type 2 diabetes, was associated with progression from mild cognitive impairment to probable Alzheimer's disease. PMID: 29338921
Study found an inverse association between the genetic risk off schizophrenia based on 108 genome-wide significantly associated SNPs and the prevalence for treated migraine in a general population sample. This association was primary linked to SNPs associated with genes encoding proteins involved in glutamatergic neurotransmission and could be attributed to the single intronic variant rs4523957 in SRR. PMID: 27394076
Data suggest that Ser-84 and Arg-135 are important in catalysis and substrate specificity of SRR. PMID: 28696262
Magnesium and calcium ions differentially affect human serine racemase activity and modulate its quaternary equilibrium toward a tetrameric form PMID: 28089597
MiR-193a-3p and miR-193a-5p play important roles in osteosarcoma metastasis through down-regulation of the Rab27B and SRR genes and therefore may serve as useful biomarkers for the diagnosis of osteosarcoma PMID: 26913720
Loss-of-function mutation of the gene encoding serine racemase significantly attenuates excitotoxicity in retina. PMID: 26485193
Serine racemase activity and dynamics are regulated by halides, ATP and malonate. PMID: 25331425
In serine racemase, similarly to the related enzyme alanine racemase, the unprotonated pyridoxal-5'-phosphate -substrate intermediate is stabilized mostly due to solvation effects contributed by water molecules and active-site residues. PMID: 25493718
FBXO22 protein is required for optimal synthesis of NMDA receptor coagonist D-serine by interacting with serine racemase, activating it, and preventing its targeting to membranes. PMID: 25336657
cross-talk between allosteric and active sites, leading to the stabilization of two alternative protein conformations with ATP affinities of ~ 10 muM and 1.8 mm PMID: 23992455
S84A serine racemase mutant behaved like serine dehydratase, whereas A65S serine dehydratase mutant acquired an additional function of using D-serine as a substrate. PMID: 23112234
The structural characteristics of SR obtained from live cells suggest that SR is sensitive to oxidation in vivo, perhaps consistent with a scenario in which such modification plays a role in feedback or other forms of regulation. PMID: 22151352
Serine racemase and D-serine are involved in both pre-symptomatic and progressive phases of amyotrophic lateral sclerosis, demonstrating a link between mutant superoxide dismutase (SOD)1 and a glial-derived toxic mediator in transgenic mice. PMID: 22117694
The SRR mRNA is elevated in people death with suicide. PMID: 20385472
The structure of mammalian serine racemase: evidence for conformational changes upon inhibitor binding PMID: 20106978
Data report on the isolation of a cDNA encoding a human serine racemase (SRR) from a human neuronal like cell line. PMID: 15193426
D-serine is synthesized in human placenta by the racemization of L-serine by serine racemase. PMID: 15219883
serine racemase catalyzes the degradation of cellular D-serine itself, through the alpha,beta-elimination of water PMID: 15536068
The frequency of the genotypes showed that 5'-G/C serine racemase is not a major risk factor for schizophrenia. PMID: 16446740
Expression of serine racemaseusing Western blot analysis in postmortem hippocampus and cortex in schizophrenia and a comparison group. PMID: 16837850
Not associated with schizophrenia in a Gefman case-control study. PMID: 17413455
Not associated with bipolar disorder in a German case-control study. PMID: 17413456
observed activation of serine racemase by divalent cations has been assumed to be a side-effect associated with ATP binding, which is known to form a complex with Mg(2+) ions PMID: 17697119
serine racemase and D-amino acid oxidase are expressed in human brain and demonstrate aberrant D-serine metabolism in schizophrenia PMID: 17880399
Analysis of SRR genetic variants in humans identified a robust association with schizophrenia. PMID: 19483194

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Protein Families

Serine/threonine dehydratase family

Tissue Specificity

Brain: expressed at high levels in hippocampus and corpus callosum, intermediate levels in substantia nigra and caudate, and low levels in amygdala, thalamus, and subthalamic nuclei. Expressed in heart, skeletal muscle, kidney and liver.

Database Links

HGNC: 14398

OMIM: 606477

KEGG: hsa:63826

STRING: 9606.ENSP00000339435

UniGene: Hs.461954

Code	CSB-EP022691HU
Abbreviation	Recombinant Human SRR protein
MSDS	MSDS Datasheet
Size	US$306
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Serine racemase (SRR)

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Target Background

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