Recombinant Human Serum response factor(SRF)

In Stock
Code CSB-YP022659HU
Size US$694
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 85% as determined by SDS-PAGE.
Target Names SRF
Uniprot No. P11831
Research Area Cardiovascular
Alternative Names c fos serum response element binding factor; c fos serum response element binding transcription factor; ELK3; ERP; MCM 1; MCM1; OTTHUMP00000039820; SAP2; Serum response factor; SRF; SRF serum response factor c fos serum response element binding transcription factor; SRF_HUMAN
Species Homo sapiens (Human)
Source Yeast
Expression Region 1-508aa
Target Protein Sequence MLPTQAGAAAALGRGSALGGSLNRTPTGRPGGGGGTRGANGGRVPGNGAGLGPGRLEREAAAAAATTPAPTAGALYSGSEGDSESGEEEELGAERRGLKRSLSEMEIGMVVGGPEASAAATGGYGPVSGAVSGAKPGKKTRGRVKIKMEFIDNKLRRYTTFSKRKTGIMKKAYELSTLTGTQVLLLVASETGHVYTFATRKLQPMITSETGKALIQTCLNSPDSPPRSDPTTDQRMSATGFEETDLTYQVSESDSSGETKDTLKPAFTVTNLPGTTSTIQTAPSTSTTMQVSSGPSFPITNYLAPVSASVSPSAVSSANGTVLKSTGSGPVSSGGLMQLPTSFTLMPGGAVAQQVPVQAIQVHQAPQQASPSRDSSTDLTQTSSSGTVTLPATIMTSSVPTTVGGHMMYPSPHAVMYAPTSGLGDGSLTVLNAFSQAPSTMQVSHSQVQEPGGVPQVFLTASSGTVQIPVSAVQLHQMAVIGQQAGSSSNLTELQVVNLDTAHSTKSE
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 53.6 kDa
Protein Length Full Length
Tag Info N-terminal 6xHis-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). Required for cardiac differentiation and maturation.
Gene References into Functions
  1. miR-647 functions as a tumor metastasis suppressor in gastric cancer by targeting SRF/MYH9 axis. PMID: 28900514
  2. Findings indicate that miR-101-3p suppresses HOTAIR-induced proliferation and invasion through directly targeting SRF in gastric carcinoma cells. PMID: 28251884
  3. promote GC metastasis by facilitating myofibroblast-cancer cell crosstalk PMID: 27323859
  4. High SRF expression is associated with breast cancer. PMID: 26885614
  5. Results identified SRF as one of the transcription factors responsible for docetaxel-resistant prostate cancer where docetaxel treatment increases its transcriptional activity. Its knockdown re-sensitizes resistant cells to docetaxel. PMID: 28249598
  6. NANOG enabled reactivation of the ROCK and Transforming Growth Factor (TGF)-beta pathways-both of which were impaired in senescent cells-leading to ACTIN polymerization, MRTF-A translocation into the nucleus and serum response factor (SRF)-dependent myogenic gene expression. PMID: 27350449
  7. HOTAIR is regulated by the RhoC-MRTF-A-SRF signaling pathway in breast cancer cells. PMID: 28069441
  8. The findings of the work indicate a role for Galphaq and/or Galpha14 and in CCR2a/CCR2b-stimulated Rho A GTPase-mediated serum response factor activation. PMID: 26823487
  9. Blood pressure-associated polymorphism controls ARHGAP42 expression via serum response factor DNA binding PMID: 28112683
  10. Subset of cellular variants of myofibroma and myopericytoma showing a smooth muscle-like immunophenotype and harboring recurrent SRF-RELA gene fusions, which mimic sarcomas with myogenic differentiation. PMID: 28248815
  11. We identified that SRF was a novel target gene for miR-22 by luciferase reporter assay and knockdown of SRF could cause endothelial dysfunction. PMID: 28161397
  12. miR-181a/b is one of the factors involved in VSMC differentiation toward a synthetic phenotype through targeting at SR PMID: 27911586
  13. miR-483-3p is upregulated in Endothelial progenitor cells (EPCs) from deep vein thrombosis patients, and it targets SRF to decrease EPCs migration and tube formation. PMID: 26801758
  14. ADGRG2 constitutively activates RhoA-SRE pathways. PMID: 26321231
  15. FLNA functions as a positive cellular transducer linking actin polymerization to MKL1-SRF activity, counteracting the known repressive complex of MKL1 and monomeric G-actin. PMID: 26554816
  16. The SRF-IL6 axis is the critical mediator of YAP-induced stemness in mammary epithelial cells and breast cancer. PMID: 26671411
  17. Data show that microRNA miR-320a is a key regulator of rtherogenesis, and down-regulating serum response factor (SRF). PMID: 25728840
  18. These data support a central role of the SRF/MRTF pathway in the pathobiology of lung fibrosis. PMID: 25681733
  19. STAT3 protein regulates vascular smooth muscle cell phenotypic switch by interaction with myocardin and SRF. PMID: 26100622
  20. high levels of Myc engage Miz1 in repressive DNA binding complexes and suppress an SRF-dependent transcriptional program that supports survival of epithelial cells. PMID: 25896507
  21. SRF regulates neutrophil migration, integrin activation, and trafficking. Disruption of the SRF pathway results in myelodysplasia and immune dysfunction. PMID: 25402621
  22. Methylation changes of GFRA1, SRF, and ZNF382 may be a potential biomarker set for prediction of gastric carcinoma metastasis. PMID: 25009298
  23. SRF promotes gastric cancer metastasis and the epithelial to mesenchymal transition through miR-199a-5p-mediated downregulation of E-cadherin. PMID: 25080937
  24. Studied a new therapeutic alternative in the treatment of multiresistant lung adenocarcinoma via siRNA-specific transfection of six crucial molecules involved in lung carcinogenesis: SFR, E2F1, Survivin, HIF1, HIF2 and STAT3. PMID: 24627437
  25. These results suggest that SRF is critical for HCC to acquire a mesenchymal phenotype, which leads to resistance against a sorafenib-mediated apoptotic effect. PMID: 24173109
  26. Dilated cardiomyopathy-associated FHOD3 variant impairs the ability to induce activation of transcription factor serum response factor. PMID: 24088304
  27. results demonstrate that serum response factor (SRF) nuclear expression in castration-resistant prostate cancer bone metastases is associated with survival with patients with shortest survival showing high SRF nuclear expression and patients with longest survival having low SRF nuclear expression PMID: 24249383
  28. Androgen-responsive SRF target genes affect CaP cell behavior. PMID: 23576568
  29. The RhoA signaling axis, a well known upstream stimulator of SRF action that harbors drugable targets, conveyed androgen-responsiveness to SRF. PMID: 23469924
  30. Transfection of the NSCLC cell lines with specific siRNAs against SRF, E2F1 and survivin resulted in a very considerable reduction of the intracellular mRNA concentration. PMID: 23152437
  31. Itk enhances Galpha13 mediated activation of serum response factor (SRF) transcriptional activity dependent on its ability to interact with Galpha13, but its kinase activity is not required to enhance SRF activity. PMID: 23454662
  32. Substitution of any of the TFBS from our particular search of MEF2, CREB and SRF significantly decreased the number of identified clusters PMID: 23382855
  33. High expression of serum response factor is associated with gastric carcinoma. PMID: 23134219
  34. Upon neuronal injury via facial nerve transection, constitutively active SRF enhances motorneuron survival. PMID: 22537405
  35. Dysfunction or loss of the SRF-activating mitogen-associated kinase pathway under stress conditions in transgenic mice may be part of Parkinson's disease etiology. PMID: 22356487
  36. Lmod1 is a new SMC-restricted SRF/MYOCD target gene. PMID: 22157009
  37. Downregulation of the activity of the MRTF-SRF axis and the expression of muscle-specific microRNAs, particularly miR-1, may contribute to COPD-associated skeletal muscle dysfunction. PMID: 21998125
  38. overexpression of serum response factor in hepatocellular carcinoma may play an important role in tumor cell migration and invasion through upregulation of matrix metalloproteinase-2 and matrix metalloproteinase-9 PMID: 21842128
  39. RTVP-1 plays a role in the effect of serum response factor on glioma cell migration PMID: 21777672
  40. Data show that serum response factor (SRF) is one of miR-483-5p target genes. PMID: 21893058
  41. SRF is able to gain nuclear entry through an auxiliary, nuclear localization sequence-independent mechanism PMID: 21131446
  42. These data define serum response factor as a host cell transcription factor that regulates immediate early gene expression in Toxoplasma-infected cells. PMID: 21479245
  43. Serum response factor up-regulation, combined with the presence of co-morbidities, increase the risk of recurrent gastric ulcer bleeding. PMID: 21410985
  44. SRF pathway alterations are linked to insulin resistance, may contribute to type 2 diabetes pathogenesis, and could represent therapeutic targets PMID: 21393865
  45. Data show that the ancestor sequence of SRF- and MEF2-type MADS domains is more similar to MEF2-type MADS domains than to SRF-type MADS domains. PMID: 20724380
  46. Overexpression of SRF in hepatocellular carcinoma (HCC) cells modulates the Wnt/beta-catenin pathway, and this plays an important role in HCC progression. PMID: 20811705
  47. Data reveal that serum response factor is a novel interferon (IFN)gamma-regulated gene and further elucidate the molecular pathway between IFNgamma, IFNgamma-regulated genes, and SRF and its target genes. PMID: 20685657
  48. two transcription factors, SRF and TFAP2, as well as an intronic element encompassing EGR3-like sequence, that work together to regulate expression of the FXN gene PMID: 20808827
  49. SRF depletion affects the expansion of the high and low differentiation grade HCC cells HepG2 and JHH6 PMID: 20144681
  50. Data show that serum response factor is an essential regulator of primary human vascular smooth muscle cell proliferation and senescence. PMID: 20096952

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Subcellular Location Nucleus
Database Links

HGNC: 11291

OMIM: 600589

KEGG: hsa:6722

STRING: 9606.ENSP00000265354

UniGene: Hs.520140

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