Recombinant Human Sialic acid-binding Ig-like lectin 15 (SIGLEC15), partial

Code CSB-EP761623HU
MSDS
Size $224
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
SIGLEC15
Uniprot No.
Research Area
Others
Alternative Names
CD33 antigen-like 3; CD33 molecule-like 3; CD33L3; HsT1361; Sialic acid-binding Ig-like lectin 15; SIG15_HUMAN; Siglec-15; SIGLEC15
Species
Homo sapiens (Human)
Source
E.coli
Expression Region
20-263aa
Target Protein Sequence
FVRTKIDTTENLLNTEVHSSPAQRWSMQVPPEVSAEAGDAAVLPCTFTHPHRHYDGPLTAIWRAGEPYAGPQVFRCAAARGSELCQTALSLHGRFRLLGNPRRNDLSLRVERLALADDRRYFCRVEFAGDVHDRYESRHGVRLHVTAAPRIVNISVLPSPAHAFRALCTAEGEPPPALAWSGPALGNSLAAVRSPREGHGHLVTAELPALTHDGRYTCTAANSLGRSEASVYLFRFHGASGAST
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
42.6kDa
Protein Length
Extracellular Domain
Tag Info
N-terminal 6xHis-SUMO-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description

This Recombinant Human SIGLEC15 protein is a powerful resource for researchers investigating sialic acid-binding Ig-like lectin 15 (Siglec-15). Known for its roles in various biological processes, including immune regulation and cell adhesion, SIGLEC15 is a valuable target for scientific exploration in both health and disease.

Our Recombinant Human SIGLEC15 protein is expressed in E.coli, resulting in the production of the extracellular domain (20-263aa). This protein features an N-terminal 6xHis-SUMO tag, allowing for efficient purification and detection while maintaining native structure and function. With a purity greater than 90% as determined by SDS-PAGE, this Recombinant Human SIGLEC15 protein delivers the reliability and consistency essential for your research efforts. Choose between liquid and lyophilized powder formats to best accommodate your experimental requirements.

Customer Reviews and Q&A

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Target Background

Function
Binds sialylated glycoproteins.
Gene References into Functions
  1. Siglec-15 recognizes the tumoral sTn antigen and transduces a signal for enhanced TGF-beta secretion in TAMs PMID: 23035012
Subcellular Location
Membrane; Single-pass type I membrane protein.
Protein Families
Immunoglobulin superfamily, SIGLEC (sialic acid binding Ig-like lectin) family
Tissue Specificity
Expressed in macrophage and/or dendritic cells of spleen and lymph nodes.
Database Links

HGNC: 27596

KEGG: hsa:284266

STRING: 9606.ENSP00000374125

UniGene: Hs.287692

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