Recombinant Human Sialic acid-binding Ig-like lectin 7 (SIGLEC7), partial

Code CSB-YP896485HU
MSDS
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Source Yeast
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Code CSB-EP896485HU
MSDS
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Source E.coli
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Code CSB-EP896485HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP896485HU
MSDS
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Source Baculovirus
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Code CSB-MP896485HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SIGLEC7
Uniprot No.
Alternative Names
Adhesion inhibitory receptor molecule 1; Adhesion inhibitory receptor molecule 1, siglec 7; AIRM 1; AIRM-1; AIRM1; CD328; CD328 antigen; CDw328; D siglec; D-siglec; HLDA 8; p75; p75/AIRM1; QA79; QA79 membrane protein; Sialic acid binding Ig like lectin 7; Sialic acid binding immunoglobulin like lectin 7; Sialic acid-binding Ig-like lectin 7; SIGL7_HUMAN; Siglec-7; SIGLEC19P; SIGLEC7; SIGLECP2
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3- and alpha-2,6-linked sialic acid. Also binds disialogangliosides (disialogalactosyl globoside, disialyl lactotetraosylceramide and disialyl GalNAc lactotetraoslylceramide). The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Mediates inhibition of natural killer cells cytotoxicity. May play a role in hemopoiesis. Inhibits differentiation of CD34+ cell precursors towards myelomonocytic cell lineage and proliferation of leukemic myeloid cells (in vitro).
Gene References into Functions
  1. Data reported that Siglec-7 was expressed on beta-cells and down-regulated in type 1 and type 2 diabetes and in infiltrating activated immune cells. Furthermore, Siglec-7 seems to play a critical role in the maintenance of an immune-suppressive anti-inflammatory microenvironment, which is lost in diabetes, and may contribute to the manifestation and progression of this metabolic syndrome. PMID: 28378743
  2. NK cells with Siglec-7neg phenotype possess a high and sustainable killing activity and have the capability to eliminate a NK-resistant leukemia cell and hypersialylated tumor cells PMID: 29617289
  3. CD56bright NK cells from obese subjects had a reduced expression of Siglec-7 PMID: 28786023
  4. Siglec-7 defines a highly functional natural killer cell subset and inhibits cell-mediated activities. PMID: 27312286
  5. Siglec-7 plays a significant role in inhibiting IgE-mediated mast cell activation, but only moderately affects IgE-mediated activation in primary human basophils. PMID: 24810846
  6. These results show that Siglec-7 binds HIV-1 via gp120 and contributes to enhance the susceptibility to infection of CD4 positive T cells and monocyte-derived macrophages. PMID: 24330394
  7. Expression of Siglec-7 and -9 ligands was associated with susceptibility of NK cell-sensitive tumor cells and, unexpectedly, of presumably NK cell-resistant tumor cells to NK cell-mediated cytotoxicity. PMID: 24569453
  8. Based on upregulated SIGLEC7 expression in blood monocytes during relapse, findings suggest a role for SIGLEC7 in acute disease activity in multiple sclerosis. PMID: 22933622
  9. Siglec-7 participates in generating a monocyte-mediated inflammatory outcome following pathogen recognition. PMID: 23029261
  10. Regulation of myeloid cell proliferation and survival by p75/AIRM1 and CD33 surface receptors. PMID: 11774609
  11. high resolution crystal structures of this protein; insights into ligand specificity of this family of proteins PMID: 12438315
  12. Sialidase unmasking of siglec-7 on NK cells permits it to interact with GD3 on target cells and inhibit killing. This has inplications for tumor escape from NK cells. PMID: 12778482
  13. x-ray crystallographic analysis of the saccharide-binding domain of p75/AIRM1 PMID: 14747738
  14. Siglec-7 and Siglec-9 are capable of negative regulation of TCR signaling and ligand binding is required for optimal activity PMID: 15292262
  15. Siglec-7 can inhibit Fc epsilon receptor I-mediated serotonin release from rat basophilic leukemia cells and recruit the tyrosine phosphatases SHP-1 and SHP-2. PMID: 15557178
  16. upon pervanadate (PV) treatment, Siglec-7 recruited the protein tyrosine phosphatases Src homology-2 (SH2) domain-containing protein-tyrosine phosphatase-1 and SHP-2 less efficiently than did other inhibitory receptors PMID: 15703304
  17. structural analysis of siglec-7 in complex with sialosides PMID: 16623661
  18. Siglecs-7 did not interact with sulfate derivatives of LacNAc and sulfated oligosaccharides containing sialic acid. PMID: 16732727
  19. The expression levels of Siglec-7 were detectable in bone marrow plasma from AML patients and serum from normal donors. PMID: 16828866
  20. The species-specific differences in the expression expression of Siglecs in SIV infection was studied. PMID: 18331725
  21. The decreased expression of Siglec-7 represents an early marker of dysfunctional natural killer-cell subsets associated with high levels of HIV-1 viremia. PMID: 19710502

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Subcellular Location
Membrane; Single-pass type I membrane protein.
Protein Families
Immunoglobulin superfamily, SIGLEC (sialic acid binding Ig-like lectin) family
Tissue Specificity
Predominantly expressed by resting and activated natural killer cells and at lower levels by granulocytes and monocytes. High expression found in placenta, liver, lung, spleen, and peripheral blood leukocytes.
Database Links

HGNC: 10876

OMIM: 604410

KEGG: hsa:27036

STRING: 9606.ENSP00000323328

UniGene: Hs.655393

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