Purity
Greater than 95% as determined by SDS-PAGE.
Greater than 95% as determined by SEC-HPLC.
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Activity
①Measured by its binding ability in a functional ELISA. Immobilized GH1 at 1 μg/ml can bind human PRLR(CSB-MP018727HU1), the EC50 of the protein is 60.71-69.65 ng/ml.②Measured by its binding ability in a functional ELISA. Immobilized GH1 at 1 μg/ml can bind human GHR(CSB-MP009411HU), the EC50 of the protein is 19.28-25.29 ng/ml.③Human GH1 protein his/myc tag (CSB-MP009407HU) captured on COOH chip can bind Human GHR protein Fc tag (CSB-MP009411HU) with an affinity constant of 6.1 nM as detected by LSPR Assay.④Measured by its binding ability in a functional ELISA. Immobilized human GH1 at 2 μg/ml can bind Biotinylated human GHR (CSB-MP009411HUj1-B), the EC50 is 2.067-3.208 ng/ml.
Research Area
Developmental Biology
Alternative Names
gH; GH-N; GH1; GHB5; GHN; Growth hormone 1; Growth hormone; Growth hormone B5; Growth hormone; normal; Growth hormone; pituitary; HG1; hGH-N; IGHD1B; Pituitary growth hormone; RNGHGP; SOMA_HUMAN; Somatotropin
Molecular Characterization
Species
Homo sapiens (Human)
Expression Region
27-217aa
Target Protein Sequence
FPTIPLSRLFDNAMLRAHRLHQLAFDTYQEFEEAYIPKEQKYSFLQNPQTSLCFSESIPTPSNREETQQKSNLELLRISLLLIQSWLEPVQFLRSVFANSLVYGASDSNVYDLLKDLEEGIQTLMGRLEDGSPRTGQIFKQTYSKFDTNSHNDDALLKNYGLLYCFRKDMDKVETFLRIVQCRSVEGSCGF
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 10xHis-tagged and C-terminal Myc-tagged
Form
Liquid or Lyophilized powder
Buffer
Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
Basically, we can dispatch the products out in 1-3
working days after receiving your orders. Delivery time may differ from different purchasing way or
location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with
normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance
and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The Recombinant Human Somatotropin GH1 is a recombinant hormone formed in mammalian cells. A DNA sequence encoding the growth hormone 1 (Phe21-Phe217) was fused with a polyhistidine tag at N-terminal and a Myc-tag at C-terminal. This sequence codes for the protein with 191 amino acids, and has a calculated molecular mass of 27.2 kDa (191 amino acids).
Human Somatotropin/Growth Hormone belongs to the growth factor family of hormones. It is secreted and stored in the anterior pituitary gland. It stimulates growth by aiding the uptake of amino acids, facilitating protein synthesis, and stimulating cellular proliferation. Most of its effects are accomplished through IGF-1.
Deletions and mutations in the gene coding for growth hormone lead to growth hormone deficiency. Growth hormone deficiency, in turn, plays a part in a couple of diseases.
The Recombinant Human Somatotropin GH1 finds application in studies involving growth hormone deficiency, Kowarski disease, Turner syndrome, cancer, metabolic disorders, and congestive heart failure.
It has an endotoxin content lower than 1 EU/µg and purity over 90%, as verified by LAL and SDS-PAGE, respectively.
