Name: Recombinant Human Somatotropin (GH1) (Active)
Brand: CUSABIO
SKU: CSB-MP009407HU

Product Details

Purity

Greater than 95% as determined by SDS-PAGE.
Greater than 95% as determined by SEC-HPLC.

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Activity

①Measured by its binding ability in a functional ELISA. Immobilized GH1 at 1 μg/ml can bind human PRLR(CSB-MP018727HU1), the EC50 of the protein is 60.71-69.65 ng/ml.②Measured by its binding ability in a functional ELISA. Immobilized GH1 at 1 μg/ml can bind human GHR(CSB-MP009411HU), the EC50 of the protein is 19.28-25.29 ng/ml.③Human GH1 protein his/myc tag (CSB-MP009407HU) captured on COOH chip can bind Human GHR protein Fc tag (CSB-MP009411HU) with an affinity constant of 6.1 nM as detected by LSPR Assay.④Measured by its binding ability in a functional ELISA. Immobilized human GH1 at 2 μg/ml can bind Biotinylated human GHR (CSB-MP009411HUj1-B), the EC₅₀ is 2.067-3.208 ng/ml.

Target Names

GH1

Uniprot No.

P01241

Research Area

Developmental Biology

Alternative Names

gH; GH-N; GH1; GHB5; GHN; Growth hormone 1; Growth hormone; Growth hormone B5; Growth hormone; normal; Growth hormone; pituitary; HG1; hGH-N; IGHD1B; Pituitary growth hormone; RNGHGP; SOMA_HUMAN; Somatotropin

Molecular Characterization

Species

Homo sapiens (Human)

Source

Mammalian cell

Expression Region

27-217aa

Target Protein Sequence

FPTIPLSRLFDNAMLRAHRLHQLAFDTYQEFEEAYIPKEQKYSFLQNPQTSLCFSESIPTPSNREETQQKSNLELLRISLLLIQSWLEPVQFLRSVFANSLVYGASDSNVYDLLKDLEEGIQTLMGRLEDGSPRTGQIFKQTYSKFDTNSHNDDALLKNYGLLYCFRKDMDKVETFLRIVQCRSVEGSCGF

Mol. Weight

27.2 kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder

Buffer

Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The Recombinant Human Somatotropin GH1 is a recombinant hormone formed in mammalian cells. A DNA sequence encoding the growth hormone 1 (Phe21-Phe217) was fused with a polyhistidine tag at N-terminal and a Myc-tag at C-terminal. This sequence codes for the protein with 191 amino acids, and has a calculated molecular mass of 27.2 kDa (191 amino acids).
Human Somatotropin/Growth Hormone belongs to the growth factor family of hormones. It is secreted and stored in the anterior pituitary gland. It stimulates growth by aiding the uptake of amino acids, facilitating protein synthesis, and stimulating cellular proliferation. Most of its effects are accomplished through IGF-1.
Deletions and mutations in the gene coding for growth hormone lead to growth hormone deficiency. Growth hormone deficiency, in turn, plays a part in a couple of diseases.
The Recombinant Human Somatotropin GH1 finds application in studies involving growth hormone deficiency, Kowarski disease, Turner syndrome, cancer, metabolic disorders, and congestive heart failure.
It has an endotoxin content lower than 1 EU/µg and purity over 90%, as verified by LAL and SDS-PAGE, respectively.

Code	CSB-MP009407HU
Abbreviation	Recombinant Human GH1 protein (Active)
MSDS	MSDS Datasheet
Size	$138
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Activity Measured by its binding ability in a functional ELISA. Immobilized GH1 at 1 μg/ml can bind human PRLR(CSB-MP018727HU1), the EC₅₀ of the protein is 60.71-69.65 ng/ml. Biological Activity Assay Activity Measured by its binding ability in a functional ELISA. Immobilized GH1 at 1 μg/ml can bind human GHR(CSB-MP009411HU), the EC₅₀ of the protein is 19.28-25.29 ng/ml. Biological Activity Assay Human GH1 protein his/myc tag (CSB-MP009407HU) captured on COOH chip can bind Human GHR protein Fc tag (CSB-MP009411HU) with an affinity constant of 6.1 nM as detected by LSPR Assay. Biological Activity Assay Activity Measured by its binding ability in a functional ELISA. Immobilized human GH1 at 2 μg/ml can bind Biotinylated human GHR (CSB-MP009411HUj1-B), the EC₅₀ is 2.067-3.208 ng/ml. Biological Activity Assay The purity of GH1 was greater than 95% as determined by SEC-HPLC
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Recombinant Human Somatotropin (GH1) (Active)

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