Recombinant Human TATA box-binding protein-like protein 1 (TBPL1)

Code CSB-YP023240HU
MSDS
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Source Yeast
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Code CSB-EP023240HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP023240HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP023240HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
TBPL1
Uniprot No.
Alternative Names
21 kDa TBP-like protein; 21kDa TBP like protein; Second TBP of unique DNA; Second TBP of unique DNA protein; STUD; TATA box binding protein like protein 1; TATA box binding protein-related factor 2; TATA box-binding protein-like protein 1; TATA box-binding protein-related factor 2; TBP like 1; TBP like protein; TBP related factor 2; TBP-like factor; TBP-like protein 1; TBP-related factor 2; TBP-related protein; tbpl1; TBPL1_HUMAN; TLF; TLP; TRF2
Species
Homo sapiens (Human)
Expression Region
1-186
Target Protein Sequence
MDADSDVALD ILITNVVCVF RTRCHLNLRK IALEGANVIY KRDVGKVLMK LRKPRITATI WSSGKIICTG ATSEEEAKFG ARRLARSLQK LGFQVIFTDF KVVNVLAVCN MPFEIRLPEF TKNNRPHASY EPELHPAVCY RIKSLRATLQ IFSTGSITVT GPNVKAVATA VEQIYPFVFE SRKEIL
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Part of a specialized transcription system that mediates the transcription of most ribosomal proteins through the 5'-TCT-3' motif which is a core promoter element at these genes. Seems to also mediate the transcription of NF1. Does not bind the TATA box.
Gene References into Functions
  1. TLP Prevents p53 Degradation through Disrupting the p53-MDM2 Interaction. PMID: 28082682
  2. The interaction of TBP-like protein with TFIIA is essential to suppress proteasome-dependent turnover of TBP-like protein PMID: 27696626
  3. miR18a exhibits a protective role in colorectal cancer (CRC) via inhibiting proliferation, invasion and migration of CRC cells by directly targeting the TBPL1 gene. PMID: 26398009
  4. results suggest that activation of the p21 upstream promoter is mediated by interaction between specific regions of TLP and p53 PMID: 24594805
  5. Western blotting and immunohistochemistry both indicated a higher TBPL1 protein expression level in colorectal cancer tissue. siRNA for TBPL1 transfection obviously slowed down the cell proliferation in both SW-620 and HT-29 cells. PMID: 24870791
  6. TLP mediates p53-governed transcriptional activation of the p21 upstream promoter. PMID: 22511763
  7. Data show that during interphase, endogenous or exogenously expressed TRF2 is located almost exclusively in the nucleolus PMID: 15269281
  8. TLF is a functional regulator of transcription with targets distinct from those of TBP. PMID: 15767669

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Subcellular Location
Cytoplasm. Nucleus.
Protein Families
TBP family
Tissue Specificity
Ubiquitously expressed, with highest levels in the testis and ovary.
Database Links

HGNC: 11589

OMIM: 605521

KEGG: hsa:9519

STRING: 9606.ENSP00000237264

UniGene: Hs.486507

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