Recombinant Human TATA box-binding protein-like protein 1 (TBPL1), partial

Code CSB-EP023240HU
MSDS
Size $224
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
TBPL1
Uniprot No.
Research Area
Epigenetics and Nuclear Signaling
Alternative Names
21 kDa TBP-like protein; 21kDa TBP like protein; Second TBP of unique DNA; Second TBP of unique DNA protein; STUD; TATA box binding protein like protein 1; TATA box binding protein-related factor 2; TATA box-binding protein-like protein 1; TATA box-binding protein-related factor 2; TBP like 1; TBP like protein; TBP related factor 2; TBP-like factor; TBP-like protein 1; TBP-related factor 2; TBP-related protein; tbpl1; TBPL1_HUMAN; TLF; TLP; TRF2
Species
Homo sapiens (Human)
Source
E.coli
Expression Region
1-185aa
Target Protein Sequence
MDADSDVALDILITNVVCVFRTRCHLNLRKIALEGANVIYKRDVGKVLMKLRKPRITATIWSSGKIICTGATSEEEAKFGARRLARSLQKLGFQVIFTDFKVVNVLAVCNMPFEIRLPEFTKNNRPHASYEPELHPAVCYRIKSLRATLQIFSTGSITVTGPNVKAVATAVEQIYPFVFESRKEI
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
36.8kDa
Protein Length
Partial
Tag Info
N-terminal 6xHis-SUMO-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

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Target Background

Function
Part of a specialized transcription system that mediates the transcription of most ribosomal proteins through the 5'-TCT-3' motif which is a core promoter element at these genes. Seems to also mediate the transcription of NF1. Does not bind the TATA box.
Gene References into Functions
  1. TLP Prevents p53 Degradation through Disrupting the p53-MDM2 Interaction. PMID: 28082682
  2. The interaction of TBP-like protein with TFIIA is essential to suppress proteasome-dependent turnover of TBP-like protein PMID: 27696626
  3. miR18a exhibits a protective role in colorectal cancer (CRC) via inhibiting proliferation, invasion and migration of CRC cells by directly targeting the TBPL1 gene. PMID: 26398009
  4. results suggest that activation of the p21 upstream promoter is mediated by interaction between specific regions of TLP and p53 PMID: 24594805
  5. Western blotting and immunohistochemistry both indicated a higher TBPL1 protein expression level in colorectal cancer tissue. siRNA for TBPL1 transfection obviously slowed down the cell proliferation in both SW-620 and HT-29 cells. PMID: 24870791
  6. TLP mediates p53-governed transcriptional activation of the p21 upstream promoter. PMID: 22511763
  7. Data show that during interphase, endogenous or exogenously expressed TRF2 is located almost exclusively in the nucleolus PMID: 15269281
  8. TLF is a functional regulator of transcription with targets distinct from those of TBP. PMID: 15767669

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Subcellular Location
Cytoplasm. Nucleus.
Protein Families
TBP family
Tissue Specificity
Ubiquitously expressed, with highest levels in the testis and ovary.
Database Links

HGNC: 11589

OMIM: 605521

KEGG: hsa:9519

STRING: 9606.ENSP00000237264

UniGene: Hs.486507

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