Purity
Greater than 90% as determined by SDS-PAGE.
Alternative Names
Alteplase; DKFZp686I03148; Plasminogen activator tissue; Plasminogen activator tissue type; PLAT; Reteplase; t PA; T Plasminogen Activator; t-PA; T-plasminogen activator; Tissue plasminogen activator (t PA); Tissue type plasminogen activator; Tissue-type plasminogen activator chain B; tPA; TPA_HUMAN; TPA1
Species
Homo sapiens (Human)
Expression Region
311-556aa
Target Protein Sequence
IKGGLFADIASHPWQAAIFAKHRRSPGERFLCGGILISSCWILSAAHCFQERFPPHHLTVILGRTYRVVPGEEEQKFEVEKYIVHKEFDDDTYDNDIALLQLKSDSSRCAQESSVVRTVCLPPADLQLPDWTECELSGYGKHEALSPFYSERLKEAHVRLYPSSRCTSQHLLNRTVTDNMLCAGDTRSGGPQANLHDACQGDSGGPLVCLNDGRMTLVGIISWGLGCGQKDVPGVYTKVTNYLDWI
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Tag Info
N-terminal 6xHis-SUMO-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
Tris-based buffer,50% glycerol
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
CUSABIO team inserts the gene coding for the Human PLAT protein (314-556aa) into a plasmid vector to form recombinant plasmid, which is then introduced into e.coli cells. e.coli cells demonstrating successful uptake of the recombinant plasmid are selected based on their ability to survive in the presence of a specific antibiotic. The positive e.coli cells are cultured under conditions that promote the expression of the gene of interest. A N-terminal 6xHis-SUMO tag is linked to the protein. Following expression, affinity purification is used to isolate and purify the recombinant Human PLAT protein from the cell lysate. Denaturing SDS-PAGE is then applied to resolve the resulting recombinant Human PLAT protein, demonstrating a purity greater than 90%.
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