Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

TLR7

Uniprot No.

Q9NYK1

Research Area

Epigenetics and Nuclear Signaling

Alternative Names

PRO285; TLR 7; Tlr7; TLR7_HUMAN; Toll like receptor 7; Toll-like receptor 7; UNQ248

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

861-1049aa

Target Protein Sequence

HLYFWDVWYIYHFCKAKIKGYQRLISPDCCYDAFIVYDTKDPAVTEWVLAELVAKLEDPREKHFNLCLEERDWLPGQPVLENLSQSIQLSKKTVFVMTDKYAKTENFKIAFYLSHQRLMDEKVDVIILIFLEKPFQKSKFLQLRKRLCGSSVLEWPTNPQAHPYFWQCLKNALATDNHVAYSQVFKETV
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

29.5 kDa

Protein Length

Partial

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Amino acids 861-1049 form the expressed segment for recombinant Human TLR7. The expected molecular weight for the TLR7 protein is calculated to be 29.5 kDa. This TLR7 recombinant protein is manufactured in e.coli. The TLR7 coding gene included the N-terminal 10xHis tag and C-terminal Myc tag, which simplifies the detection and purification processes of the recombinant TLR7 protein in following stages of expression and purification.

Toll-like receptor 7 (TLR7) is a critical component of the innate immune system, belonging to the Toll-like receptor (TLR) family. TLR7 is predominantly expressed in immune cells, such as dendritic cells and macrophages, and is located within endosomal compartments. It plays a key role in recognizing single-stranded RNA from viruses. Upon binding to its ligands, TLR7 activates signaling pathways that involve the adaptor protein MyD88, leading to the activation of transcription factors like NF-κB and the production of pro-inflammatory cytokines, interferons, and other immune mediators. TLR7 is especially important in antiviral responses, contributing to the host defense against viral infections. Research on TLR7 aims to elucidate its signaling cascades, identify specific ligands, and understand its role in various diseases. Additionally, TLR7 agonists are explored for their potential in vaccine development and immunotherapy against viral infections and certain cancers.

Target Background

Function

Endosomal receptor that plays a key role in innate and adaptive immunity. Controls host immune response against pathogens through recognition of uridine-containing single strand RNAs (ssRNAs) of viral origin or guanosine analogs. Upon binding to agonists, undergoes dimerization that brings TIR domains from the two molecules into direct contact, leading to the recruitment of TIR-containing downstream adapter MYD88 through homotypic interaction. In turn, the Myddosome signaling complex is formed involving IRAK4, IRAK1, TRAF6, TRAF3 leading to activation of downstream transcription factors NF-kappa-B and IRF7 to induce proinflammatory cytokines and interferons, respectively.

Gene References into Functions

Enhanced TLR7 expression owing to biallelism contributes to the higher risk of developing SLE. PMID: 29374079
Btk acts in the TLR7/8 pathway and mediates Ser-536 phosphorylation of p65 RelA and subsequent nuclear entry in primary human macrophages. PMID: 29567473
the miRNA profile did not significantly differ between SLE and APS, but was driven by the presence or absence of an IFN signature. TLR7 stimulation induced a general downregulation of miRNAs, similar to the pattern observed in SLE and APS patients. PMID: 29873766
TLR7 upregulation has a role in liver cancer cell proliferation involving lipid rafts PMID: 27588480
Study demonstrates that Hepatitis C virus (HCV) genomic RNA harbours specific sequences that initiate an anti-HCV immune response through TLR7 and TLR8 in various antigen presenting cells. PMID: 27385120
TLR7, TLR9, and JAK2 genes are potential biomarkers for systemic sclerosis. High TLR7 expression positively correlated with the late form of disease. Decreased levels of TLR9 and JAK2 mRNA were found in the patient's cohort in comparison to non-SSc individuals. PMID: 29147913
The results indicate that TLR7 up-regulation is related to Respiratory syncytial virus infection and the induction of oxidative stress and that TLR7 expression was mediated by the anti-inflammatory effects of Nrf2/ARE pathway inhibitors or agonists. PMID: 29392496
Thus, TLR7 and TLR8 might modulate different immune responses in monocytes and macrophages. PMID: 29448098
Positive rates of iNOS in cervical tissues were 72.1%, 28.2%, and 3.1% in the -HPV-positive patients with cervical cancer (CC group), HR-HPV group, and controls, respectively (P < 0.05). Levels of TLR3, TLR4, TLR7, TLR8, NF-kappaB p65, and iNOS in cervical epithelial cells were higher in CC group than in other groups. PMID: 28626766
Data suggest a central role of XBP1 in TLR7-induced IFNalpha production and identify XBP1 as a potential novel therapeutic target in IFNalpha-driven autoimmune and inflammatory diseases. PMID: 28408069
These findings suggest that increased EV71 and CA16 replication meditated by autophagy in 16HBE cells might promote degradation of the endosome, leading to suppression of the TLR7-mediated IFN-I signaling pathway. PMID: 29052054
The present study investigated the effects of vitamin D3 on the expression of TLR3, TLR7, and TLR9 in Systemic lupus erythematosus patients. PMID: 28544067
findings indicate that hepatitis C virus induces CD4 T cell impairment via TLR7 which may contribute to failure of virus eradication, casting doubts on the use of TLR7 agonists to boost innate immunity in chronic RNA virus infections. PMID: 27876497
TLR 5, 7, and 9 expression patterns differed between HPV-positive and -negative oropharyngeal squamous cell carcinoma patients. In HPV-positive tumors the expression of TLR 5 and 7 correlated with tumor recurrence. PMID: 28856441
Low TLR7 copy number is a risk factor for chronic hepatitis B virus infection but is not associated with later stages of disease progression PMID: 28321161
TLR7 and TLR8 genetic polymorphisms are associated with susceptibility to mycobacterium tuberculosis infection, and the link is shaped by less effective MTB phagocytosis and impaired TLR signaling. PMID: 27156628
Study evaluated innate immune profiles following TLR stimulation in HIV-1-infected mothers and newborns, found significantly compromised cytokine responses upon extracellular and intracellular TLR activation. Myeloid dendritic cell (DC) responsiveness appeared to be less impaired than plasmacytoid DCs, and might be enhanced through TLR7/TLR8 activation. PMID: 23826189
This is the first study illustrating certain genotypes of TLR-7 and TLR-8 single nucleotide polymorphisms viz. CT(p = 0.002)]; rs3853839[GC(p < 0.001), CC(p = 0.039)] and rs3764879[GC(p < 0.001)] were considerably associated with Chikungunya virus susceptibility. PMID: 28888110
study demonstrates that activation of TLR7 signaling in T cells can inhibit Th17 cell differentiation from naive T cells and IL-17 production in established Th17 cells; further report that downregulation of STAT3 signaling is responsible for TLR7-mediated inhibition of Th17 cells due to induction of suppressor of cytokine signaling 3 and 5 PMID: 28652396
Data indicate a mechanism in which Toll-like receptors TLR7/8 signaling, through shedding of FcgRIIA, shifts neutrophil function from phagocytosis to a programmed necrosis pathway, neutrophil extracellular trap formation (NETosis). PMID: 28606989
TLR7 is abundantly expressed in human atherosclerotic plaques. TLR7 ligation elicits the secretion of pro-inflammatory and anti-inflammatory cytokines, and high TLR7 expression in plaques is associated with better patient outcome. PMID: 27864310
this study identified TLR7 as mediator of monocyte differentiation and M2 macrophage polarization during hepatitis C virus infection PMID: 28122964
Increased susceptibility to autoimmune is associated with single nucleotide polymorphisms and copy number variations of Toll like receptor 7. [review] PMID: 28397237
COPD lung tissue explants showed a greater pro-inflammatory response to TLR3 or TLR7/8 activation than control smokers. PMID: 27729782
the activation of TLR7 increased CCND3 expression via the downregulation of miR-15b in B cells. PMID: 26144250
TLR9 and TLR7 were upregulated both at the mRNA and the protein levels in wounds of type 2 diabetes mellitus patients compared with the non-diabetic patients and may lead to an unresolved inflammatory response and non-healing chronic ulcers. PMID: 25586463
This study suggests that deliberate control of TLR signaling is a key factor in the success of mRNA-driven cellular reprogramming. PMID: 27586271
changes in mRNA expression of selected toll-like receptors (TLR2, TLR4, TLR7), stress cytokine prolactin (PRL), and proand anti-inflammatory cytokines (TNF-alpha, IL-6, IL-12, IL-10) in peripheral blood monocytes of celiac disease patients, were investiagted. PMID: 28028951
The ER-stress inducing property of IMQ is possibly of importance for its efficacy in treating basal cell carcinoma, in situ melanoma, and squamous cell carcinoma. Our data could potentially be harnessed for rational design of even more potent ER-stress inducers and new anti-cancer drugs. PMID: 27003259
TLR8 polymorphisms were associated with an increased risk and TLR7 polymorphisms with a decreased risk of recurrent rhinovirus infections. PMID: 28403045
We found lower expression levels of TLR1, TLR3, TLR4, TLR7 and TLR9 in PBMCs from patients with ALL compared with those from control patients. We also observed that the PBMCs from patients with Pre-B and B ALL had lower TLR4 expression than controls PMID: 27277333
interferon alpha (IFN-alpha) secretion induced by Toll-like receptor 7 and Toll-like receptor 8 (TLR7/TLR8) activation was observed in common variable immunodeficiency (CVID), which was recovered with Toll-like receptor 9 (TLR9) signaling. PMID: 27392462
this study shows that TLR7 gene polymorphism is associated with susceptibility to HIV and HCV co-infection PMID: 28062211
TLR7 gene rs2897827 may influence TLR7 mRNA expression and the plasma ApoA1 level in male ischemic stroke patients. PMID: 27427388
Considering that chronic hepatitis B infection is not yet curable, it could be possible to activate TLR7-related immunological pathways as a therapy directed towards persistent HBV infection PMID: 27373425
Both TLR 5 and 7 are expressed in salivary adenoid cystic carcinoma on the cell membranes as well as in cytoplasm. PMID: 26888781
human Toll-like receptor 7 ligand binding domain PMID: 25817271
TLR7 mRNA expression was significantly elevated in patients with relapsing remitting multiple sclerosis. PMID: 26996115
Our meta-analysis suggests that TLR7, TLR8, and TLR9 polymorphisms are associated with the development of systemic lupus erythematosus in Caucasian, Asian, and African populations PMID: 26762473
activation of TLR7 upregulated the expression levels of IFN-lambda1 and MMP-9 were increased by ~3 fold, whereas other genes (p53, PTEN, TIMP-1) were upregulated by ~2 fold, and VEGF was marginally upregulated after 10 min. PMID: 26718740
Increased expression of TLR7 and TLR9 in myasthenia gravis thymus is accompanied by active Epstein-Barr virus infection. PMID: 26723518
The low expression of TLR-7 in tumour and high expression of TLR-7 in stroma predict a good clinical outcome for oral squamous cell carcinoma patients PMID: 25828894
TLR7 and 8 polymorphisms may play a considerable role in the pathogenesis of asthma. PMID: 26725554
NADPH oxidase-deficient patient-derived B cells also expressed enhanced levels of TLR7 and TLR9 mRNA and protein compared with the same cells reconstituted to restore oxidase activity. PMID: 26340429
Data show that differences in codon bias limit Toll-like receptor 7 (TLR7) expression relative to Toll-like receptor 9 (TLR9). PMID: 26903634
Activation of TLR7 suppresses the progression of pancreatic cancer. PMID: 26238718
Results show that TLR7 and TLR8 on trophoblastic cells play an important role in the prevention of intrauterine HBV transmission by inhibiting HBV translocation across trophoblasts. PMID: 26315138
In females, the incidence of the X-linked TLR7 rs179008/Gln11Leu polymorphism was significantly lower in sarcoidosis patients. PMID: 24071890
A key regulatory cytokine found increased upon TLR7 stimulation. PMID: 25923141
This study established a correlation between miR-155 and TLR7 during hepatitis B virus infection and also demonstrated in vitro that increased miR-155 level could help to reduce viral load by targeting C/EBP-beta. PMID: 25720442

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Subcellular Location

Endoplasmic reticulum membrane; Single-pass type I membrane protein. Endosome. Lysosome. Cytoplasmic vesicle, phagosome.

Protein Families

Toll-like receptor family

Tissue Specificity

Detected in brain, placenta, spleen, stomach, small intestine, lung and in plasmacytoid pre-dendritic cells. Expressed in peripheral mononuclear blood cells.

Database Links

HGNC: 15631

OMIM: 300365

KEGG: hsa:51284

STRING: 9606.ENSP00000370034

UniGene: Hs.659215

Code	CSB-EP023606HU
Abbreviation	Recombinant Human TLR7 protein, partial
MSDS	MSDS Datasheet
Size	$306
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Toll-like receptor 7 (TLR7), partial

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