Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

TNFAIP6

Uniprot No.

P98066

Research Area

Cancer

Alternative Names

Hyaluronate binding protein; Hyaluronate-binding protein; TNF alpha induced protein 6; TNF alpha-induced protein 6; TNF stimulated gene 6 protein; TNF-stimulated gene 6 protein; TNFAIP 6; Tnfaip6; TSG 6; TSG-6; TSG6_HUMAN; Tumor necrosis factor alpha induced protein 6; Tumor necrosis factor alpha inducible protein 6; Tumor necrosis factor alpha-induced protein 6; Tumor necrosis factor inducible gene 6 protein; Tumor necrosis factor stimulated gene 6 protein; Tumor necrosis factor-inducible gene 6 protein

Species

Homo sapiens (Human)

Source

Baculovirus

Expression Region

18-277aa

Target Protein Sequence

WGFKDGIFHNSIWLERAAGVYHREARSGKYKLTYAEAKAVCEFEGGHLATYKQLEAARKIGFHVCAAGWMAKGRVGYPIVKPGPNCGFGKTGIIDYGIRLNRSERWDAYCYNPHAKECGGVFTDPKQIFKSPGFPNEYEDNQICYWHIRLKYGQRIHLSFLDFDLEDDPGCLADYVEIYDSYDDVHGFVGRYCGDELPDDIISTGNVMTLKFLSDASVTAGGFQIKYVAMDPVSKSSQGKNTSTTSTGNKNFLAGRFSHL
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The recombinant Human TNFAIP6 was expressed with the amino acid range of 18-277. This TNFAIP6 protein is expected to have a theoretical molecular weight of 33 kDa. The TNFAIP6 protein was expressed in baculovirus. The N-terminal 10xHis tag and C-terminal Myc tag was fused into the coding gene segment of TNFAIP6, making it easier to detect and purify the TNFAIP6 recombinant protein in the later stages of expression and purification.

TNFAIP6 (Tumor necrosis factor-inducible gene 6 protein) is a protein of significant research interest. The primary focus of research lies in its role in inflammation and immune regulation. TNFAIP6 plays a crucial role in regulating gene expression, cell proliferation, and tumor development. Researchers investigate its expression in inflammatory conditions like rheumatoid arthritis and inflammatory bowel disease, along with its potential involvement in tumor development and treatment resistance. These studies contribute to revealing the molecular mechanisms of TNFAIP6 in the onset and progression of diseases, providing a theoretical basis for the treatment of related conditions.

Target Background

Function

Possibly involved in cell-cell and cell-matrix interactions during inflammation and tumorigenesis.

Gene References into Functions

TSG-6 up-regulation counteracts the progression of atherosclerosis. (Review) PMID: 29401724
TSG-6 from MSCs attenuated severe burn-induced excessive inflammation via inhibiting activation of P38 and JNK signaling. PMID: 27444207
TSG-6 secreted by human mesenchymal stem cells suppresses inflammatory reactions through p38 and ERK in the mitogen-activated protein kinase pathway PMID: 28247086
This review will focus on the potential of mesenchymal stem/stromal cells for treatment of fibrotic diseases, with emphasis on the role of TSG-6 as a mediator of anti-inflammatory effects PMID: 26807758
abnormally elevated in both the plasma and aortic wall of patients with abdominal aortic aneurysms PMID: 29180197
Single nucleotide polymorphism in TNFAIP6 gene is associated with Systemic lupus erythematosus. PMID: 28246883
TNFAIP6 expression is significantly upregulated in human masticatory mucosa during wound healing PMID: 28005267
Data show that TNF-stimulated gene-6 (TSG-6) interacts with chemokines through their glycosaminoglycans (GAGs)-binding sites and inhibits their binding to GAGs and endothelial cell surfaces. PMID: 27044744
Data postulate that the molecular cross-linking enhanced by the multiple binding modes of the Link module might be critical for remodeling the ECM during inflammation/ovulation and might contribute to other functions of TSG-6. PMID: 26685054
the well characterized hyaluronan-binding site in the TSG-6 Link module is not used for recognition during transfer of HCs onto HA. PMID: 26468290
TSG-6 exhibited anti-inflammatory effects during the wound healing process and cicatrization and significantly diminished hypertrophic scar formation in a rabbit ear model PMID: 25661977
results suggest that systemic administration of hASC or TSG-6 may be novel approaches to reverse CS-induced myelosuppression. PMID: 25329668
TSG-6 activity in tissues, was examined. PMID: 25325979
TSG-6 expression by hMSCs was induced 12 h after oropharyngeal delivery to LPS-exposed lungs. Knockdown of TSG-6 expression in hMSCs by RNA interference abrogated most of their anti-inflammatory effects. PMID: 21569482
Heavy chain transfer by tumor necrosis factor-stimulated gene 6 to the bikunin proteoglycan. PMID: 25561734
data suggest that TSG-6 expression might be essential in endometrial matrix organization and feto-maternal communication during the implantation process. PMID: 24213015
the HA-binding site defined here may not play a role in TSG-6-mediated transfer of heavy chains from inter-alpha-inhibitor onto HA, a process known to be essential for ovulation. PMID: 24403066
TSG-6 suppresses CXCL8-mediated chemotaxis of neutrophils; this lower potency effect might be important at sites where there is high local expression of TSG-6. PMID: 24501198
This study provides insight into what we believe to be a previously undescribed multifaceted role of mesenchymal stem cell-released TSG-6 in wound healing. PMID: 23921952
Data indicate that TSG-6-mediated cross-linking of hyaluronan (HA) films is impaired in the presence of inter-alpha-inhibitor (IalphaI) and that this effect suppresses the TSG-6-mediated enhancement of HA binding to CD44-positive cells. PMID: 24005673
The protein product of tumor necrosis factor-alpha stimulated gene-6 strongly correlated with ossification markers and hyaluronidase in calcified human aortic valves. PMID: 23017666
This review discusses TSG-6 as a potential molecular marker of oocyte maturation. PMID: 22922154
TSG-6 increases the accumulation of HA in the cell-associated matrix, partially by preventing its dissolution from the cell-associated matrix into the conditioned medium, but primarily by inducing HA synthesis PMID: 23129777
analysis of irreversible heavy chain transfer to hyaluronan oligosaccharides by tumor necrosis factor-stimulated gene-6 PMID: 23166324
TSG-6 reverts the inhibitory effects exerted by PTX3 on FGF2-mediated angiogenesis through competition of FGF2/PTX3 interaction. PMID: 22267482
TSG-6 was central to EMT through effects on HA macromolecular structure and through CD44-dependent triggering of cell responses. PMID: 21864707
TSG-6 is a potent HA cross-linking agent PMID: 21596748
TSG-6 protein, a negative regulator of inflammatory arthritis, forms a ternary complex with murine mast cell tryptases and heparin. PMID: 21566135
negative correlation between TSG-6 expression levels and severity of capsular contracture (CC) suggests a possible protective role for TSG-6 in the context of CC formation, and this may have a clinically relevant role in prevention of breast CC PMID: 21239672
TSG-6/HC2 link heavy chains randomly on the chondroitin sulfate chain of bikunin, in contrast to the ordered attachment observed during the biosynthesis. PMID: 20463016
polymorphism in osteoarthritis; chromosome location given here (chromosome 2) PMID: 11854277
TSG-6 is a multifunctional protein associated with inflammation [review] PMID: 12692188
Tumor necrosis factor, alpha-induced protein 6 activates an anti-inflammatory agent- inter-alpha-inhibitor[review]. PMID: 14515153
The TSG-6 and I alpha I interaction promotes a transesterification cleaving the protein-glycosaminoglycan-protein (PGP) cross-link. PMID: 15653696
This TSG-6 model reveals the residues most likely to be involved in stabilizing the inter-Link module interface, which are highly conserved across the Link protein/ chondroitin sulfate proteoglycan subfamily. PMID: 15718240
TSG-6 treatment also resulted in a rapid increase in cyclooxygenase-2 (COX-2) mRNA levels, suggesting that TSG-6 up-regulates COX-2 gene expression PMID: 15809059
TSG-6 acts as cofactor and catalyst in the production of IalphaI heavy chain x hyaluronan complexes PMID: 15840581
heparin with the Link module significantly increases the anti-plasmin activity of the TSG-6.IalphaI complex; TSG-6 is likely to contribute to matrix remodeling, at least in part, through down-regulation of the protease network PMID: 15917224
The tumor necrosis factor alpha induced protein 6 is a potential marker that could be used for early diagnosis and prognosis of cancerous or precancerous lesions. PMID: 16467113
hyaluronan synthase 2 and TSG-6 have roles in hyaluronan distribution and function in proximal tubular epithelial cells PMID: 16687630
Evidence is cited in this review that TSG-6 can regulate the expression of various molecules that have important roles in the control of inflammation. PMID: 16709183
TSG-6 is down-regulated during osteoblastic differentiation, and suppressed osteoblastic differentiation induced by both BMP-2 and osteogenic differentiation medium. PMID: 16771708
TSG-6 may play an important protective role in bronchial epithelium by increasing the antiprotease screen on the airway lumen. PMID: 16873769
Crystallography data on the free protein, and (15)N NMR relaxation data for the uncomplexed and HA(8)-bound forms of Link_TSG6, is presented. PMID: 17585936
TSG-6 is a high affinity ligand that can mediate fibronectin interactions with other matrix components and modulate some interactions of fibronectin with cells PMID: 18042364
3 genes were upregulated in patients with chronic EBV infection: guanylate binding protein 1, tumor necrosis factor-induced protein 6, and guanylate binding protein 5; they may be associated with the inflammatory reaction or with cell proliferation. PMID: 18260761
The transfer of heavy chains from bikunin proteins to hyaluronan requires both TSG-6 and HC2. PMID: 18448433
TSG-6 has dual roles in bone remodeling: it inhibits RANKL-induced bone erosion in inflammatory diseases, and its interactions with BMP-2 and RANKL help to balance mineralization by osteoblasts and bone resorption by osteoclasts PMID: 18586671
TSG-6 transfers proteins between glycosaminoglycans via a Ser28-mediated covalent catalytic mechanism PMID: 18820257
myelomonocytic cells and MoDC are a major source of TSG-6 and that PTX3 and TSG-6 are coregulated PMID: 19389798

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Tissue Specificity

Found in the synovial fluid of patients with rheumatoid arthritis.

Database Links

HGNC: 11898

OMIM: 600410

KEGG: hsa:7130

STRING: 9606.ENSP00000243347

UniGene: Hs.437322

Code	CSB-BP023959HU
Abbreviation	Recombinant Human TNFAIP6 protein
MSDS	MSDS Datasheet
Size	$528
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Tumor necrosis factor-inducible gene 6 protein (TNFAIP6)

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