Recombinant Human UDP-glucose:glycoprotein glucosyltransferase 2 (UGGT2), partial

Code CSB-YP865182HU
MSDS
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Source Yeast
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Code CSB-EP865182HU
MSDS
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Source E.coli
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Code CSB-EP865182HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP865182HU
MSDS
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Source Baculovirus
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Code CSB-MP865182HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
UGGT2
Uniprot No.
Alternative Names
UGGT2; UGCGL2; UGT2UDP-glucose:glycoprotein glucosyltransferase 2; UGT2; hUGT2; EC 2.4.1.-; UDP--Glc:glycoprotein glucosyltransferase 2; UDP-glucose ceramide glucosyltransferase-like 1
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Recognizes glycoproteins with minor folding defects. Reglucosylates single N-glycans near the misfolded part of the protein, thus providing quality control for protein folding in the endoplasmic reticulum. Reglucosylated proteins are recognized by calreticulin for recycling to the endoplasmic reticulum and refolding or degradation.
Gene References into Functions
  1. In this study, we aimed to clarify the contribution of the noncatalytic domains by comparing activities of truncated forms of recombinant HUGT1/HUGT2 and HUGT1/HUGT2 chimeras with full-length enzymes. PMID: 27496766
  2. The glucosyltransferase activity of UGGT2 has been lacking, leaving it unclear as to whether it has any function in the glycoprotein folding process. PMID: 24415556
  3. HUGT2 cannot activate the catalytic domain of UDP-glucose: glycoprotein glucosyltransferase I PMID: 12913004
Subcellular Location
Endoplasmic reticulum lumen. Endoplasmic reticulum-Golgi intermediate compartment.
Protein Families
Glycosyltransferase 8 family
Tissue Specificity
Higher levels in kidney, pancreas, heart, and skeletal muscle.
Database Links

HGNC: 15664

OMIM: 605898

KEGG: hsa:55757

STRING: 9606.ENSP00000365938

UniGene: Hs.193226

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