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The full-length cDNA ORF (1-449aa) of human Wilms tumor protein(WT1) was N-terminally tagged a 6xHis-SUMO and then expressed in E.coli. The Recombinant Human WT1 thus obtained was purified using SDS-PAGE analysis and got over 90% of purity. Under reducing conditions, the WT1 protein migrated to an approximately 66 kDa molecular mass band on the SDS-PAGE gel. In addition to being an immunogen for specific antibody generation, this recombinant WT1 protein also may be used in the WT1-associated transcription studies.
WT1 is a transcription factor indispensable for the development of urogenital, epicardium, and kidney during embryogenesis. In mammals, WT1 is expressed in specific tissues and has distinct expression at the different developmental stages. Studies have shown that WT1 exerts dual functions in tumorigenesis, that is, WT1 acts as a tumor suppressor or executes a proto-oncogenic effect. Inactivation or ablation of WT1 leads to the loss of specific target gene binding, interfering with the normal regulation of transcription, cell growth, differentiation, and proliferation, and eventually results in tumorigenesis. Also, WT1 may facilitate cell growth and take part in tumor cell infiltration and metastasis by modulating the transcriptional expression of growth factor genes.
|Purity||Greater than 90% as determined by SDS-PAGE.|
WIT 2; WT 1; AWT1; FWT1; GUD; NPHS4; WAGR; Wilms tumor 1; Wilms Tumor; Wilms tumor protein; Wilms' tumor gene; Wilms' tumor protein; WIT2; WT; WT1; WT1_HUMAN; WT33
|Species||Homo sapiens (Human)|
|Target Protein Sequence||MGSDVRDLNALLPAVPSLGGGGGCALPVSGAAQWAPVLDFAPPGASAYGSLGGPAPPPAPPPPPPPPPHSFIKQEPSWGGAEPHEEQCLSAFTVHFSGQFTGTAGACRYGPFGPPPPSQASSGQARMFPNAPYLPSCLESQPAIRNQGYSTVTFDGTPSYGHTPSHHAAQFPNHSFKHEDPMGQQGSLGEQQYSVPPPVYGCHTPTDSCTGSQALLLRTPYSSDNLYQMTSQLECMTWNQMNLGATLKGVAAGSSSSVKWTEGQSNHSTGYESDNHTTPILCGAQYRIHTHGVFRGIQDVRRVPGVAPTLVRSASETSEKRPFMCAYPGCNKRYFKLSHLQMHSRKHTGEKPYQCDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGKTSEKPFSCRWPSCQKKFARSDELVRHHNMHQRNMTKLQLAL
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
|Protein Length||Full Length|
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
|Buffer|| If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
|Reconstitution||We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.|
|Storage Condition||Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.|
|Shelf Life||The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
|Lead Time||3-7 business days|
|Notes||Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.|
|Datasheet & COA||Please contact us to get it.|
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Transcription factor that plays an important role in cellular development and cell survival. Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3'. Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has lower affinity for DNA, and can bind RNA.
|Gene References into Functions||
|Involvement in disease||Frasier syndrome (FS); Wilms tumor 1 (WT1); Denys-Drash syndrome (DDS); Nephrotic syndrome 4 (NPHS4); Meacham syndrome (MEACHS); Mesothelioma, malignant (MESOM)|
|Subcellular Location||Nucleus. Nucleus, nucleolus. Cytoplasm.; [Isoform 1]: Nucleus speckle.; [Isoform 4]: Nucleus, nucleoplasm.|
|Protein Families||EGR C2H2-type zinc-finger protein family|
|Tissue Specificity||Expressed in the kidney and a subset of hematopoietic cells.|