Code | CSB-RA950156A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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WB | 1:500-1:5000 |
IHC | 1:50-1:200 |
The WT1 recombinant monoclonal antibody is produced through a four-step process. First, the WT1 monoclonal antibody gene is sequenced, and then the gene is cloned into a plasmid vector. Next, the recombinant vector is introduced into a host cell line. The WT1 recombinant monoclonal antibody is then purified from the cell culture supernatant using affinity chromatography, followed by testing and characterizing the purified antibody. The WT1 monoclonal antibody is developed using a synthesized peptide derived from human WT1 as the immunogen. This WT1 recombinant monoclonal antibody is suitable for detecting human WT1 protein using ELISA, WB, and IHC applications.
The WT1 is a transcription factor that plays an important role in the development and function of various tissues in the body, particularly in the formation and function of the kidneys and gonads. In the kidneys, WT1 is involved in the development of the nephrons, which are the functional units of the kidneys responsible for filtering blood and producing urine. In the gonads, WT1 is involved in the development of the testes and ovaries and is required for the proper differentiation and function of the Sertoli cells in the testes and granulosa cells in the ovaries. WT1 has also been shown to play a role in the regulation of gene expression, cell proliferation, and apoptosis in various tissues, including the kidney, gonads, and hematopoietic systems. Abnormal expression or mutations in the WT1 gene have been associated with a variety of diseases, including Wilms tumor, Denys-Drash syndrome, and Frasier syndrome.
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