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Recombinant Human papillomavirus type 18 Protein E7 (E7)

Recombinant Human papillomavirus type 18 Protein E7 (E7)

^{In Stock}

Code	CSB-YP361948HMN
Abbreviation	Recombinant Human papillomavirus type 18 E7 protein
MSDS	MSDS Datasheet
Size	$250
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

Uniprot No.

P06788

Research Area

Epigenetics and Nuclear Signaling

Alternative Names

E7; Protein E7

Species

Human papillomavirus type 18

Source

Yeast

Expression Region

1-105aa

Target Protein Sequence

MHGPKATLQDIVLHLEPQNEIPVDLLCHEQLSDSEEENDEIDGVNHQHLPARRAEPQRHTMLCMCCKCEARIKLVVESSADDLRAFQQLFLNTLSFVCPWCASQQ
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

14.0 kDa

Protein Length

Full Length

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Tris-based buffer,50% glycerol

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Customer Reviews and Q&A

■ Customer Reviews

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■ Q&A

We are using the E7 HPV protein from Lot# 03541 and need to change to a buffer without glycerol in order to make our experiments work. Is this possible? Or is the protein not H2O soluble?

Thanks for your inquiry.
1. It is suggested that you can change the buffer by yourselves, by performing dialysis. This experiment is very simple and easy to operate.
You can perform dialysis according to the storage buffer in our Datasheet&COA, WITHOUT glycerol, ie 20 mM Tris-HCl, 0.5 M NaCl, pH 8.0, to dialyze with a solution of the appropriate volume.
However, there will be certain loss of protein after the dialysis process.
2. We haven't detected the stability of the protein in water. In general, proteins will be stable at a certain ion concentration and pH buffering conditions,
but we have also seen a protein that can be stably present in the water environment before (The stability of this protein also has not been detected yet).
3. If you do not have the appropriate conditions to deal with, you can also re-order the protein without glycerol. If you have interest, we will send you the details.
Please do not hesitate to contact us if the customer has further questions. Nice day.

Target Background

Function

Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. E7 protein has both transforming and trans-activating activities. Induces the disassembly of the E2F1 transcription factor from RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes. Interferes with host histone deacetylation mediated by HDAC1 and HDAC2, leading to transcription activation. Plays also a role in the inhibition of both antiviral and antiproliferative functions of host interferon alpha. Interaction with host TMEM173/STING impairs the ability of TMEM173/STING to sense cytosolic DNA and promote the production of type I interferon (IFN-alpha and IFN-beta).

Subcellular Location

Host cytoplasm. Host nucleus.

Protein Families

Papillomaviridae E7 protein family

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