Recombinant Human papillomavirus type 18 Protein E7 (E7)

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Code CSB-EP361948HMN
Size $224
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP361948HMN could indicate that this peptide derived from E.coli-expressed Human papillomavirus type 18 E7.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP361948HMN could indicate that this peptide derived from E.coli-expressed Human papillomavirus type 18 E7.
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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
E7
Uniprot No.
Alternative Names
E7; Protein E7
Species
Human papillomavirus type 18
Source
E.coli
Expression Region
1-105aa
Target Protein Sequence
MHGPKATLQDIVLHLEPQNEIPVDLLCHEQLSDSEEENDEIDGVNHQHLPARRAEPQRHTMLCMCCKCEARIKLVVESSADDLRAFQQLFLNTLSFVCPWCASQQ
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
16.0kDa
Protein Length
Full Length
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
Tris-based buffer,50% glycerol
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description

The recombinant human papillomavirus type 18 E7 protein is a fusion protein consists of the human papillomavirus type 18 E7 protein (1-105aa) partnered with the N-terminal 6xHis tag. It was produced in the E.coli. This recombinant human papillomavirus type 18 E7 protein's urity is greater than 90% determined by SDS-PAGE. After electrophoresis, there is a 19 kDa protein band presented on the gel.

E7 proteins encoded by the cancer-associated alpha human papillomaviruses have potent transforming activities, which together with E6, are necessary but not sufficient to render their host squamous epithelial cell tumorigenic. HPV E7 proteins consist of approximately 100 amino acid residues. There are no cellular proteins that share extensive sequence similarities to E7. E7 proteins play a central role in the human papillomavirus life cycle, reprogramming the cellular environment to be conducive to viral replication. Some researches noted that the oncogenic activities of the high-risk alpha HPV E7 proteins represent functions related to the viral life cycle and/or arise as a consequence of a specific replication strategy that these viruses have adopted to establish a long-term persistent infection and/or produce viral progeny.

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 Q&A
Q:

Can you please provide the concentration of CSB-EP361948HMN; Recombinant Human papillomavirus type 18 Protein E7 (E7) to me?

A:
Thanks for your inquiry. According the the record, this protein is provdied as lyophilized form.
Lyophilized from a 0.2 μm filtered 10 mM Tris-HCl, 1 mM EDTA, pH 8.0.
The volume before lyophilization is 200ul/vial.

Target Background

Function
Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. E7 protein has both transforming and trans-activating activities. Induces the disassembly of the E2F1 transcription factor from RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes. Interferes with host histone deacetylation mediated by HDAC1 and HDAC2, leading to transcription activation. Plays also a role in the inhibition of both antiviral and antiproliferative functions of host interferon alpha. Interaction with host TMEM173/STING impairs the ability of TMEM173/STING to sense cytosolic DNA and promote the production of type I interferon (IFN-alpha and IFN-beta).
Subcellular Location
Host cytoplasm. Host nucleus.
Protein Families
Papillomaviridae E7 protein family
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