Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

Bnip3

Uniprot No.

O55003

Research Area

others

Alternative Names

Bnip3; Nip3; BCL2/adenovirus E1B 19 kDa protein-interacting protein 3

Species

Mus musculus (Mouse)

Source

E.coli

Expression Region

1-156aa

Target Protein Sequence

MSQSGEENLQGSWVELHFSNGNGSSVPASVSIYNGDMEKILLDAQHESGRSSSKSSHCDSPPRSQTPQDTNRAEIDSHSFGEKNSTLSEEDYIERRREVESILKKNSDWIWDWSSRPENIPPKEFLFKHPKRTATLSMRNTSVMKKGGIFSADFLK
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

21.6 kDa

Protein Length

Partial

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The region for expressing recombinant Mouse Bnip3 contains amino acids 1-156. The calculated molecular weight for this Bnip3 protein is 21.6 kDa. The Bnip3 protein was expressed in e.coli. The N-terminal 6xHis tag was fused into the coding gene segment of Bnip3, making it easier to detect and purify the Bnip3 recombinant protein in the later stages of expression and purification.

Bnip3 (BCL2/adenovirus E1B 19 kDa protein-interacting protein 3) is one of the proteins currently under intense scrutiny in biomedical research. One of its primary areas of investigation is in the realms of apoptosis and mitochondrial function regulation. In this field, Bnip3 has been found to be closely associated with the regulation of the cell's self-destruction process and the stability of mitochondria. Particularly, under conditions of cellular stress and hypoxia, the expression levels of Bnip3 significantly increase, thereby influencing cell survival and death. Research on Bnip3 also extends to cardiovascular diseases, especially the physiological and pathological processes of cardiac muscle cells. Its role in conditions like ischemia-reperfusion injury and myocardial infarction is of particular interest. Scientists, by studying the relationship between Bnip3 expression and cardiovascular diseases, aim to discover new therapeutic methods and interventions.

Target Background

Function

Apoptosis-inducing protein that can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP: in response to mitochondrial damage, participates in mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane may play a critical role in the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix. Plays an important role in the calprotectin (S100A8/A9)-induced cell death pathway.

Gene References into Functions

High-fat-mediated liver damage is associated with Sirt3 downregulation, which is followed by ERK-CREB pathway inactivation and Bnip3-mediated inhibition of mitophagy, causing hepatocytes to undergo mitochondria-dependent cell death. PMID: 30056271
JNK activates Mff and Bnip3, contributing to the fatal mitochondrial fission and mitophagy, respectively. PMID: 29149759
BNIP3 expression was upregulated in hypoxic keratinocytes, and BNIP3 silencing suppressed hypoxia-induced cell migration. PMID: 27783443
In a neuronal model of dominant optic atrophy, BNIP3 down-regulation reduced autophagy and mitophagy. PMID: 27861891
Down-regulation of Bcl2/adenovirus E1B 19-kDa-interacting protein 3 (BNIP3) by olomoucine, a cyclin-dependent kinasesinhibitor, reduces lipopolysaccharide - and nitric oxide-induced cell death in BV2 microglial cells. Olomoucine may protect cells by limiting proinflammatory responses, thereby reducing nitric oxide generation. PMID: 27345388
data outline Bnip3 as a key effector of PPARgamma-mediated adipose mitochondrial network fragmentation, improving insulin sensitivity and limiting oxidative stress. PMID: 27325287
the results suggest that BNIP3 plays a vital role in regulating PINK1 mitochondrial outer membrane localization, the proteolytic process of PINK1 and PINK1/parkin-mediated mitophagy under physiological conditions. PMID: 27528605
BNIP3 interacts with the mitochondrial outer membrane directly via mitochondrial BAX. PMID: 28333095
Data show that TGFbeta-activated kinase-1 (TAK1) activated nuclear factor of activated T-cells (NFAT)/NF-kappa B (NFkappaB), downregulated BCL2-adenovirus E1B interacting protein 3 (Bnip3), and inhibited cardiac cell death. PMID: 26564789
propose that BNIP3 acts as a brake on HIF-1 activity serving to increase rates of mitophagy in response to hypoxia and to limit production of damaging ROS that would further amplify HIF-1 expression and promote tumor progression to metastasis PMID: 26232272
Results suggest that Bnip3 regulates cardiac gene expression and perhaps myocyte morphology by activating nuclear p300 acetyltransferase and hyperacetylating histones and its selective transcription factors. PMID: 26317696
Bnip3 dual-functionality and crosstalk between mitophagy and apoptosis pathways is presented here. PMID: 26253153
regulates mitophagy during hypoxia, whereas NIX is required for mitophagy during development of the erythroid lineage. PMID: 25753537
role in the generation of robust NK cell memory in the process of mitophagy during viral infection PMID: 26253785
BNIP3 primarily regulates basal level of mitophagy in physiological conditions, whereas BNIP3 exclusively activates excessive mitophagy leading to cell death. PMID: 25230377
Bnip3 generation, mediated by PARP1, causes mitochondrial damage and neuron death. PMID: 25429139
Suggest pro-tumorigenic role of BNIP3 driving melanoma cell's aggressive features, like migration and vasculogenic mimicry. PMID: 24625986
BNIP3 has a protective effect against UVB-induced apoptosis in keratinocytes PMID: 24402046
The BNIP3 cell death pathway may be a new target for protecting oligodendrocytes from death after stroke PMID: 23692407
Bnip3 is a required downstream effector of FoxO-driven autophagic flux in mechanically unloaded failing myocardium. PMID: 23568341
These results uncover a mechanism of cavitation through hypoxia-induced apoptosis of the core cells mediated by HIFs, Bnip3, and AIF. PMID: 22753893
Loss of BNip3 resulted in increased lipid synthesis in the liver that was associated with elevated ATP levels, reduced AMP-regulated kinase (AMPK) activity, and increased expression of lipogenic enzymes. PMID: 22547685
our mouse model demonstrates a balance between BNIP3-mediated autophagy and H-ras(val12)-induced tumor formation and reveals that H-ras(val12) induces autophagy in a BNIP3-dependent manner. PMID: 22241963
The role of HIF-1alpha or NBIP3 in hypoxia-induced authophagy activation and osteoclastogenesis is reported. PMID: 21465467
Expression of NOV and BNIP3 in leukemia AML-M(4) is significantly higher than that in normal controls. PMID: 21518474
Suggest that induction of mitochondrial autophagy in response to Bnip3 is a protective response activated by the cardiac myocytes that involves Drp1-mediated mitochondrial fission and recruitment of Parkin. PMID: 21890690
enforced and endogenous expression of Ras coincided with the up-regulation of BNIP3 across a wide spectrum of cancer cells, providing the first experimental evidence that BNIP3 is a regulatory target of H-Ras PMID: 21868531
Bnip3 caused an increase in mitochondrial protease activity, suggesting that Bnip3 might promote degradation of proteins in the mitochondria. PMID: 21278801
findings reveal a novel intrinsic defense mechanism that opposes the mitochondrial defects and cell death of ventricular myocytes that is obligatorily linked and mutually dependent on alternative splicing of Bnip3FL during hypoxia or ischemic stress PMID: 21415393
Expression of MAFbx and Bnip3 was increased in hearts of mice in bearing colonic tumors. PMID: 21167183
Bnip3 mediates mitochondrial permeabilization by a novel mechanism that is different from other BH3-only proteins PMID: 20025887
Bnip3 is important in the cardiomyocyte death pathway after severe hypoxia. PMID: 20160671
The expression of PLAGL2 leads to the mRNA expression of a proapoptotic factor, Nip3 PMID: 11832486
Promoter analysis showed that the region between -281 and -1 of the 5'-upstream enhancer region of murine BNIP3 was sufficient for nitric oxide-dependent expression of BNIP3 PMID: 15358175
Data suggest that Bnip3-mediated upregulation of autophagic activity constitutes a protective response against Bnip3 death signaling. PMID: 16874059
Results identify BNIP3 as a key regulator of hypoxia-induced autophagy and suggest a novel role for the RB tumor suppressor in preventing nonapoptotic cell death by limiting the extent of BNIP3 induction in cells. PMID: 17576813
Bnip3/Bnip3L play a crucial role in anthrax lethal toxin-induced cytotoxicity, and down-regulation of Bnip3/Bnip3L is a mechanism of spontaneous or toxin-induced resistance of macrophages. PMID: 17623653
The study suggests that Bnip3 may actually allow cell survival either by preventing ATP depletion or by eliminating damaged mitochondria. PMID: 17786027
Bnip3 minimizes ventricular remodeling in the mouse. PMID: 17909626
These results suggest that BNIP-3 is a candidate for an intrinsic factor related to antidepressive effects and that Wakan-yaku theory may be useful for the identification of other intrinsic functional molecules. PMID: 18606473
Hepatic BNIP3 was also upregulated in two different models of liver stress in vivo, suggesting that a multitude of inflammatory stresses can lead to the modulation of BNIP3 PMID: 19147804
chronic intermittent hypoxia could up-regulate the expression of Nip3, and result in neuron apoptosis and ultrastructural changes in neurons of the frontal cortex PMID: 19187620
Increased cell injury and/or death could be caused directly by the upregulation of bNip3, a preapoptotic molecule that dimerizes with Bcl-2, or indirectly by the aberrant expression of SP-C-induced endoplasmic reticulum stress in epithelial cells. PMID: 19574421
BNIP3 is an important regulator of caspase-independent neural precursor cell death after hypoxia. PMID: 19915483

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Subcellular Location

Mitochondrion. Mitochondrion outer membrane; Single-pass membrane protein.

Protein Families

NIP3 family

Database Links

KEGG: mmu:12176

STRING: 10090.ENSMUSP00000101718

UniGene: Mm.378890

Code	CSB-EP002766MO1
Abbreviation	Recombinant Mouse Bnip3 protein, partial
MSDS	MSDS Datasheet
Size	$306
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Mouse BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 (Bnip3), partial

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