Purity
Greater than 85% as determined by SDS-PAGE.
Alternative Names
Bnip3; Nip3; BCL2/adenovirus E1B 19 kDa protein-interacting protein 3
Species
Mus musculus (Mouse)
Expression Region
1-156aa
Target Protein Sequence
MSQSGEENLQGSWVELHFSNGNGSSVPASVSIYNGDMEKILLDAQHESGRSSSKSSHCDSPPRSQTPQDTNRAEIDSHSFGEKNSTLSEEDYIERRREVESILKKNSDWIWDWSSRPENIPPKEFLFKHPKRTATLSMRNTSVMKKGGIFSADFLK
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The region for expressing recombinant Mouse Bnip3 contains amino acids 1-156. The calculated molecular weight for this Bnip3 protein is 21.6 kDa. The Bnip3 protein was expressed in e.coli. The N-terminal 6xHis tag was fused into the coding gene segment of Bnip3, making it easier to detect and purify the Bnip3 recombinant protein in the later stages of expression and purification.
Bnip3 (BCL2/adenovirus E1B 19 kDa protein-interacting protein 3) is one of the proteins currently under intense scrutiny in biomedical research. One of its primary areas of investigation is in the realms of apoptosis and mitochondrial function regulation. In this field, Bnip3 has been found to be closely associated with the regulation of the cell's self-destruction process and the stability of mitochondria. Particularly, under conditions of cellular stress and hypoxia, the expression levels of Bnip3 significantly increase, thereby influencing cell survival and death. Research on Bnip3 also extends to cardiovascular diseases, especially the physiological and pathological processes of cardiac muscle cells. Its role in conditions like ischemia-reperfusion injury and myocardial infarction is of particular interest. Scientists, by studying the relationship between Bnip3 expression and cardiovascular diseases, aim to discover new therapeutic methods and interventions.