Recombinant Mouse C-C motif chemokine 17 (Ccl17) (Active)

CCL17 orchestrates T helper 2 cell recruitment to sites of allergic inflammation and tumor microenvironments, making quantitative assessment of its chemotactic potency essential for modeling Th2-driven pathology. This mammalian-expressed construct spanning residues 24–93 demonstrates robust receptor engagement in functional ELISA, binding immobilized anti-CCL17 antibody with an EC50 of 0.98–1.49 ng/mL—a quantitative benchmark that supports dose-response studies in chemotaxis assays, T cell migration experiments, and CCR4 signaling pathway investigations including MAPK and calcium flux activation. Endotoxin levels below 1.0 EU/μg meet the contamination thresholds required for primary immune cell assays, where LPS artifacts would confound interpretation of CCL17-specific effects on Th2 differentiation or regulatory T cell function. The C-terminal mFc tag facilitates immunoprecipitation and surface plasmon resonance studies while the >95% purity provides a clean reagent for antibody development workflows, including use as coating antigen in sandwich immunoassays or as a positive control in Western blot validation of anti-CCL17 detection reagents.