Recombinant Mouse F-box/WD repeat-containing protein 1A(Btrc)

Code CSB-YP662374MO
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Source Yeast
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Code CSB-EP662374MO
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Source E.coli
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Code CSB-EP662374MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP662374MO
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Source Baculovirus
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Code CSB-MP662374MO
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Btrc
Uniprot No. Q3ULA2
Alternative Names Btrc; Fbw1; Fbxw1; Fwd1; Kiaa4123; F-box/WD repeat-containing protein 1A; Beta-TrCP protein E3RS-IkappaB; Beta-transducin repeat-containing protein; Beta-TrCP; E3RSIkappaB; mE3RS-IkappaB; F-box and WD repeats protein beta-TrCP; HOS; Ubiquitin ligase FWD1; pIkappaB-E3 receptor subunit
Species Mus musculus (Mouse)
Expression Region 1-605
Target Protein Sequence MDPAEAVLQE KALKFMCSMP RSLWLGCSSL ADSMPSLRCL YNPGTGALTA FQNSSEREDC NNGEPPRKII PEKNSLRQTY NSCARLCINQ ETVCLTSTAM KTENCVAKAK LANGTSSMIV PKQRKLSASY EKEKELCVKY FEQWSESDQV EFVEHLISQM CHYQHGHINS YLKPMLQRDF ITALPARGLD HIAENILSYL DAKSLCAAEL VCKEWYRVTS DGMLWKKLIE RMVRTDSLWR GLAERRGWGQ YLFKNKPPDE NAPPNSFYRA LYPKIIQDIE TIESNWRCGR HSLQRIHCRS ETSKGVYCLQ YDDQKIVSGL RDNTIKIWDK STLECKRILT GHTGSVLCLQ YDERVIITGS SDSTVRVWDV NAGEMLNTLI HHCEAVLHLR FNNGMMVTCS KDRSIAVWDM ASPTDITLRR VLVGHRAAVN VVDFDDKYIV SASGDRTIKV WNTSTCEFVR TLNGHKRGIA CLQYRDRLVV SGSSDNTIRL WDIECGACLR VLEGHEELVR CIRFDNKRIV SGAYDGKIKV WDLMAALDPR APAGTLCLRT LVEHSGRVFR LQFDEFQIVS SSHDDTILIW DFLNDPAAHA EPPRSPSRTY TYISR
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Data

Function Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds to phosphorylated target proteins
Gene References into Functions
  1. beta-TrCP regulates the transition from mitosis to meiosis in male germ cells by targeting DMRT1 for degradation. PMID: 28982686
  2. Data indicate a critical physiological function of beta-TRCP in regulating hepatic lipid metabolic homeostasis in part through modulating Lipin1 stability. PMID: 28049764
  3. betaTrCP levels are correlated with imiquimod-induced skin lesion. PMID: 27476970
  4. beta-TrCP1/FWD1 dominant negative mutant has a role in reducing myeloma cell growth PMID: 26009993
  5. Erbin as a novel substrate of SAG-betaTrCP E3 ligase. PMID: 26056141
  6. these results clearly suggest that both beta-TrCP- and Hrd1-dependent degradation mechanisms regulate the transcriptional activity of Nrf1 to maintain cellular homeostasis. PMID: 21911472
  7. beta-TrCP may be an essential player in UVB induced responses in skin PMID: 21187057
  8. Glycogen synthase kinase 3 phosphorylates a group of Ser residues in the Neh6 domain of mouse Nrf2 that overlap with an SCF/beta-TrCP destruction motif (DSGIS, residues 334 to 338) and promotes its degradation in a Keap1-independent manner. PMID: 21245377
  9. LPS appears to destabilize the LPCAT1 protein by GSK-3beta-mediated phosphorylation within a canonical phosphodegron for beta-TrCP docking and site-specific ubiquitination. PMID: 21068446
  10. the two beta-TrCP paralogs have a nonredundant role in spermatogenesis PMID: 20194439
  11. anti-inflammatory effects of H(2)O(2) may result from its ability to decrease ubiquitination as well as subsequent degradation of I kappaB alpha through inhibiting the association between I kappaB alpha and SCF(beta-TrCP) PMID: 19933270
  12. Results suggest a role for Btrc in regulating the eventual ratio of resulting differentiated retinal cell types. PMID: 19705444
  13. hnRNP-U engages a highly neddylated active SCF beta-TrCP which dissociates in the presence of a high-affinity substrate, resulting in the ubiquitination of the latter. PMID: 11850407
  14. HOS plays a role in inhibiting cell differentiation and cell transformation PMID: 12151397
  15. b-TrCP controls meiotic and mitotic progression in vivo. PMID: 12791266
  16. beta-TrCP1 contributes to, but is not absolutely required for, the degradation of I kappa B and beta-catenin PMID: 12843402
  17. mammary glands of betaTrcp1(-/-) female mice display a hypoplastic phenotype, whereas no effects on cell proliferation are observed in other somatic cells PMID: 15340078
  18. Cancer cell lines harboring Smad4 point mutations exhibited rapid Smad4 protein degradation due to the effect of SCF(beta-TrCP1), also called Btrc. PMID: 15855639
  19. Results indicate that the turnover of Mcl-1 by beta-TrCP is an essential mechanism for GSK-3beta-induced apoptosis and contributes to GSK-3beta-mediated tumor suppression and chemosensitization. PMID: 17387146
  20. Data show that beta-TrCP2 and beta-TrCP1 target PER2 protein in vitro, and also show that the beta-TrCP binding site of PER2 is recognized by both beta-TrCP1 and beta-TrCP2. PMID: 18782782
  21. Isoforms of beta-transducin repeat containing protein generated by alternative splicing may have different biological roles. PMID: 18929646
  22. results identify IKK2 and beta-TrCP1 as novel regulators of the p53 pathway and suggest that blocking of IKK2 and beta-TrCP1 could be a means of regulating p53 stability and thereby modulating its biological activity PMID: 19196987

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Subcellular Location Cytoplasm, Nucleus
Tissue Specificity Expressed in heart, brain, liver, skeletal muscle and, most strongly, in testis.
Database Links

KEGG: mmu:12234

STRING: 10090.ENSMUSP00000070728

UniGene: Mm.119717

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