Recombinant Mouse Glial fibrillary acidic protein(GFAP)

Code CSB-YP009369MO
Size US$1593
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names Gfap
Uniprot No. P03995
Research Area Others
Alternative Names GfapGlial fibrillary acidic protein; GFAP
Species Mus musculus (Mouse)
Source Yeast
Expression Region 1-430aa
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 51.9kDa
Protein Length Full Length
Tag Info N-terminal 6xHis-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Gene References into Functions
  1. This report the successful prediction and validation of Gfap as an miR-3099 target gene using a combination of bioinformatics resources with enrichment of annotations based on functional ontologies and a spatio-temporal expression dataset. PMID: 28597341
  2. These results indicate that autoantibodies against GFAP could serve as a predictive marker for the development of overt autoimmune diabetes. PMID: 28546444
  3. GFAP is specifically expressed in the auricular chondrocytes, and assumes a pivotal role in resistance against mechanical stress. PMID: 28063220
  4. compared open-skull and thinned-skull imaging methods for two-photon laser microscopy of live astrocytes in neocortex of GFAP-GFP transgenic mice PMID: 28107381
  5. work reveals that an Alexander disease-causing mutation alters GFAP turnover kinetics in vivo and provides an essential foundation for future studies aimed at preventing or reducing the accumulation of GFAP. PMID: 28223355
  6. Tat expression or GFAP expression led to formation of GFAP aggregates and induction of unfolded protein response (UPR) and endoplasmic reticulum (ER) stress in astrocytes. PMID: 27609520
  7. Study provides evidence that transcription of one of the astrocyte-specific genes, Gfap, is cooperatively regulated by co-expressed genes and their regulatory factors. PMID: 27041678
  8. This study demonstrated the GFAP-ApoE4 mice exhibited motor impairments when compared to GFAP-ApoE3 and wild-type mice. PMID: 26892275
  9. PINK1 deficiency causes defects in GFAP-positive astrogliogenesis during brain development. PMID: 26746235
  10. Gnasxl deficiency does not directly affect glial development in the hypothalamus, since it is expressed in neurons, and Gfap-positive astrocytes and tanycytes appear normal during early postnatal stages. PMID: 27080240
  11. Induction of glial cytokine expression was sequential, aligned with active sickness behavior, and preceded increased Iba-1 or GFAP immunoreactivity after lipopolysaccharide challenge PMID: 26470014
  12. Study provides a mechanistic link between the GFAP mutations/overexpression and the symptoms in those affected with Type II Alexander disease PMID: 26190408
  13. Study described GFAP-expressing non-myelinating Schwann cells in the lung, validated a transgenic mouse line that drives expression of cre under a GFAP promoter PMID: 26442852
  14. findings thus show that the inability to produce GFAP and Vim affects normal retinal physiology and that the effect of IF deficiency on retinal cell survival differs, depending on the underlying pathologic condition PMID: 26251181
  15. CUL4B as a negative regulator of GFAP expression during neural development. PMID: 26025376
  16. Astrocytes deficient of GFAP and vimentin showed decreased Notch signal sending competence and altered expression of Notch signaling pathway-related genes PMID: 26118771
  17. Absence of GFAP, or both GFAP and vimentin, alters Alzheimer's disease-induced changes in gene expression profile of astrocytes, showing a compensation of the decrease of neuronal support genes and a trend for a higher inflammatory expression profile PMID: 25731615
  18. Data indicate that glial fibrillary acidic protein (GFAP) was up-regulated in satellite glial cells (SGCs) in dorsal root ganglia 14 days after streptozotocin injection. PMID: 25312986
  19. Findings demonstrate that ENT1 regulates GFAP expression and possibly astrocyte function PMID: 25365803
  20. Data suggest that prenatal alterations in expression of various fetal brain proteins (including down-regulation of Gfap) are associated with aberrant behavioral characteristics of transgenic mice that model autism-like behavior. PMID: 25849768
  21. Study shows that increased levels of astrocytic GFAP can contribute to increases on inter-ictal spikes but do not represent a risk factor for appearance of post-traumatic seizures, even when there is increase in reactive gliosis PMID: 25069089
  22. The Phactr4 signals were not associated with F-actin fibers but were closely associated with intermediate filaments such as nestin and glial fibrillary acidic protein (GFAP) fibers. PMID: 24748504
  23. Report increasing glial fibrillary acidic protein expression and electron microscopic features of brain edema in rodent cerebral malaria. PMID: 24966914
  24. absence of GFAP and vimentin in glial cells does not seem to affect the outcome after peripheral motoneuron injury but may have an important effect on the response dynamics PMID: 24223940
  25. the astrocyte became activated, exhibiting significantly increased levels of GFAP expression directly related to the level of HIV/VSV replication PMID: 24254728
  26. traumatic scratch injury to astrocytes triggered a calcium influx from the extracellular compartment and activated the JNK/c-Jun/AP-1 pathway to switch on GFAP PMID: 24123203
  27. Data show that CD8 T cells reactive to glial fibrillary acidic protein (GFAP), a protein expressed in astrocytes, drive unique aspects of inflammatory central nervous system autoimmunity. PMID: 24591371
  28. Brain levels of GFAP and Tau proteins decreased significantly at 6 h and increased considerably at 24 h after repeated blast exposures. Plasma samples showed a similar initial decrease and later increase over this timeframe. PMID: 23933206
  29. Data indicate that Gfapdelta is expressed in the in developing mouse brain sub-ventricular zones in accordance with the described localization in the developing and adult human brain. PMID: 23991052
  30. GFAP was found to be downregulated in HSV-1 acute infection in cornea and upregulated in late stage, suggesting that GFAP might play some role during HSV-1 infection in cornea. PMID: 23758602
  31. Data show that neurofibromatosis type 1 (NF1)-inactivation results in a cell-autonomous increase in glial fibrillary acidic protein+ (GFAP+), but not in NG2 proteoglycan NG2+, cell proliferation in vitro. PMID: 23318450
  32. this study demonistrated that mouse models of Alexander disease exhibit significant pathology in GFAP-positive radial glia-like cells in the dentate gyrus, and suffer from deficits in adult neurogenesis. PMID: 24259590
  33. These studies demonstrate that transactivation of the Gfap promoter is an early and sustained indicator of the disease process in the mouse. PMID: 23432455
  34. These data suggest that all astroglia cells in the developing and adolescent mouse brain express GFAPdelta, regardless of their neurogenic capabilities. PMID: 23285135
  35. The GFAP-stained intensity of the retinal area is increased in contralateral eyes and decreased in retinal ganglion cells of eyes with laser-induced ocular hypertension. PMID: 22583833
  36. Postulate that glial cells with increased Gfap expression support the elongation of new neurites from retinal ganglion cells possibly by providing a scaffold for outgrowth. PMID: 23259929
  37. GFAP expression is almost not affected by melatonin treatment in aged mice. PMID: 22200709
  38. differential regulation of GFAP isoforms is not involved in the reorganization of the intermediate filament network in reactive gliosis or in neurogenesis in the mouse brain. PMID: 22912745
  39. CD44-positive cells are APCs in the early postnatal cerebellum; surviving cells gradually express glial fibrillary acidic protein GFAP), a marker for mature astrocytes, indicating differentiation into mature astrocytes is the default for these cells. PMID: 21732075
  40. GFAP-negative astrocytes are fully inflammation-competent, displaying phenotypic heterogeneity as is commonly observed in brain astrocytes. PMID: 22072312
  41. In a mouse model of amyotrophic lateral sclerosis (ALS), GFAP is not necessary for the initiation of disease; instead, it plays some modulatory roles in the progression of ALS. PMID: 21453731
  42. Treadmill exercise training decreased the expression of GFAP in the striatum of chronic Parkinsonian mice, which can partially explain the beneficial neuroprotective role of exercise in patients with parkinson disease. PMID: 21725169
  43. The study shows opposing pattern of nestin and glial fibrillary acidic protein expression in mouse hippocampus occuring in early postnatal development, suggesting that it is important for neural differentiation and positioning in the hippocampus. PMID: 21368556
  44. In the mouse there is a slight increase in the number of GFAP positive cells in the white matter after 3 days of severe cerebral contusion trauma. PMID: 20479526
  45. Increased expression of GFAP in Muller cells of mer knockout mice occur at P20d in the peripheral retina and P4w in the central retina. GFAP expression in Muller cells appears to be a secondary response to the loss of retinal neurons. PMID: 20497693
  46. One protein, GFAP (glial fibrillary acidic protein), was found to be elevated in the LINCL mice compared with normal controls in both isolated storage bodies and a lysosome-enriched subcellular fraction that contains storage material. PMID: 20370715
  47. The results of this study suggested that GFAP is necessary for morphological retention and distribution of reactive astrocytes during prion disease, and that there is a GFAP-dependent function of glial filaments in reactive astrocytes. PMID: 19931516
  48. Report demonstrated for the first time that GFAPdelta is specifically expressed in radial glia and SVZ neural progenitors during human brain development. PMID: 20040497
  49. The exact expression of glial fibrillary acidic protein (GFAP) in trigeminal ganglion and dental pulp. PMID: 11838710
  50. Human influenza viral infection in utero alters GFAP immunoreactivity in the developing brains of neonatal mice. PMID: 12140787
  51. important role for astrocytes and GFAP in the progress of ischemic brain damage and increased ICP after cerebral ischemia with reperfusion PMID: 12168322
  52. nestin-containing cells express glial fibrillary acidic protein in the proliferative regions of central nervous system of postnatal developing and adult mice PMID: 12414089
  53. Results indicate that the activation of the endogenous glial fibrillary acidic protein (GFAP) gene as a consequence of viral infection could involve different regulatory pathways than activation as a result of prion infection. PMID: 12531521
  54. Gfap was used to identify astrocytes in culture. PMID: 12837282
  55. adult forebrain neural stem cells comprise a subpopulation of the GFAP-positive cells within the subependyma PMID: 12859339
  56. Axonal plasticity and functional recovery after spinal cord injury in mice deficient in both glial fibrillary acidic protein and vimentin genes. PMID: 12861073
  57. GFAP plays a crucial role in the hippocampal neurodegeneration after central nervous system insult. PMID: 14753450
  58. Loss of GFAP results in decreases in both astrocytic (EAAT1) and neuronal (EAAT3) glutamate transporter subtypes, and a region-specific modification of neuronal glutamate transporter(EAAT3)trafficking. PMID: 15135219
  59. A role is revealed for GFAP in central nervous system infections: restricting lesion size, intracerebral pathogenic load, and in prevention of pathogen-induced tissue necrosis within the highly vulnerable brain parenchyma. PMID: 15217091
  60. The Glial Fibrillary Acidic Protein immunoreactivitity was strongly found in the periischemic area. PMID: 15647743
  61. GFAP-expressing neural stem cells are phenotypically and functionally distinct from non-neurogenic astrocytes PMID: 16267834
  62. LIF generates GFAP(+) cells that remain in cell cycle, contain progenitor cell markers, BMP-induced GFAP(+) cells are stellate, exit the cell cycle, and lack progenitor traits. PMID: 16314487
  63. GFAP protein level exhibited a 50% reduction, while its mRNA remained unaffected by rearing in darness in the visual cortex. PMID: 16343449
  64. Thus, expression of the GFAP gfa28-lacZ transgene appears to serendipitously mark a distinct set of astrocyte precursors. PMID: 16482522
  65. GFAP-expressing cells in concert with ependymal cells can perform typical astrocytic functions such as K+ and glutamate buffering in the postnatal SVZ but display a unique set of functional characteristics intermediate between astrocytes and radial glia. PMID: 16886203
  66. data show that the density of GFAP immunoreactive processes in the hippocampus peaks on proestrus although cell density does not change PMID: 16926532
  67. GFAP might contribute to form macro-complexes to initiate mitogenic and differentiating signaling for efficient nerve regeneration. PMID: 16988027
  68. analysis of glial fibrillary acidic protein, metallothionein, and MHC II expression in human, rat and mouse cells PMID: 17008879
  69. Feedback interactions among GFAP accumulation, SAPK/JNK activation, and proteasomal hypofunction cooperate to produce further protein accumulation and cellular stress responses PMID: 17038307
  70. These results indicate that GFAP plays a crucial role in pyramidal neuronal survival after injury or KA-induced neurotoxicity. PMID: 17085299
  71. These results indicate that the presence of GFAP aggregates containing mutant GFAP is not sufficient to induce a major phenotype of Alexander disease, even though it causes some abnormalities in the mouse. PMID: 17299771
  72. Dmn is expressed in reacive astrocytes in neurotrauma and interacts differentially with Vim and Gfap. PMID: 17356066
  73. These results indicate that the influenza A/NWS virus enhances the activation of astrocytes and GFAP and NOS-2 expression which in turn enhances NO production and the expansion of capillary blood vessels. PMID: 18080188
  74. GFAP upregulation in ischemic injury may have different functional significance in female and male experimental animals and may not directly reflect the extent of ischemia-induced neuronal damage in female GFAP-luc mice. PMID: 18258827
  75. These results identify the PACAP-cAMP-Ca(2+)-DREAM cascade as a new pathway to activate GFAP gene expression during astrocyte differentiation. PMID: 18579744
  76. Transgenic mice showed more severe ABR deficits and OHC damage, suggesting that cochlear nerve glial cells with GFAP aggregates play a role in noise susceptibility. Thus, we should focus more on the roles of cochlear nerve glial cells in SNHL. PMID: 18602179
  77. Data show that no migration of nestin-positive cells are immunoreactive for GFAP in the peri-infarct area after photothrombosis. PMID: 18616932
  78. In the bite raised condition, the number of neurons was lower in the dorsal and ventral CA3 subfields of the hippocampus, and the number of glial fibrillary acidic protein-labeled astrocytes was increased in the CA1, CA3, and DG subfields. PMID: 18975611
  79. The disease onset was characterized by induction of GFAP in peripheral nerve Schwann cells suggesting that peripheral nerves pathology/denervation and Schwann cell stress may play an important role in the ALS pathogenesis. PMID: 19115383
  80. Data show that mutant and excess wild type GFAP promote formation of cytoplasmic inclusions, disrupts the cytoskeleton, decreases cell proliferation, increases cell death, reduces proteasomal function, and compromises astrocyte resistance to stress. PMID: 19146851
  81. These data suggest that a pool of GFAP/S100beta+ glial cells located in the cerebellar white matter generate a large fraction of cerebellar interneurons for the molecular layer within the first postnatal 12 days of cerebellar development. PMID: 19418461
  82. The GFAP-fLuc was highly expressed in brain compared to other tissues, and was limited to astrocytes, whereas the GAPDH-RLuc was more widely expressed. PMID: 19457099

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Subcellular Location Cytoplasm
Protein Families Intermediate filament family
Tissue Specificity Brain; isoform 2 expressed at 20-fold lower level than isoform 1.
Database Links

KEGG: mmu:14580

STRING: 10090.ENSMUSP00000064691

UniGene: Mm.1239

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