Recombinant Mouse Ly6/PLAUR domain-containing protein 3 (Lypd3), partial (Active)

Lypd3, a GPI-anchored protein implicated in metastasis-associated pathways, presents challenges for functional studies due to its membrane-tethered nature and complex folding requirements. This recombinant fragment spanning residues 33–287 demonstrates specific antibody binding in functional ELISA, with an EC50 of 1.995–2.345 ng/mL when immobilized at 2 μg/mL and probed with anti-LYPD3 antibody, confirming that the protein retains native epitope presentation. Mammalian expression preserves post-translational modifications critical for proper domain folding, enabling this protein to serve as a positive control in antibody validation workflows, as an immunogen for generating research-grade antibodies against Lypd3, and as a binding partner in protein-protein interaction assays investigating Lypd3-mediated signaling in cancer models. The combination of >95% purity and <1.0 EU/μg endotoxin satisfies the quality criteria typical for cell-based binding studies and supports use in assays where low background interference is essential.