Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

Pdk1

Uniprot No.

Q8BFP9

Research Area

Signal Transduction

Alternative Names

Pdk1; [Pyruvate dehydrogenase; acetyl-transferring)] kinase isozyme 1; mitochondrial; EC 2.7.11.2; Pyruvate dehydrogenase kinase isoform 1; PDH kinase 1

Species

Mus musculus (Mouse)

Source

Baculovirus

Expression Region

27-434aa

Target Protein Sequence

ASASASGSGPASELGVPGQVDFYARFSPSPLSMKQFLDFGSVNACEKTSFMFLRQELPVRLANIMKEISLLPDNLLRTPSVQLVQSWYIQSLQELLDFKDKSAEDAKTIYEFTDTVIRIRNRHNDVIPTMAQGVTEYKESFGVDPVTSQNVQYFLDRFYMSRISIRMLLNQHSLLFGGKGSPSHRKHIGSINPNCDVVEVIKDGYENARRLCDLYYVNSPELELEELNAKSPGQTIQVVYVPSHLYHMVFELFKNAMRATMEHHADKGVYPPIQVHVTLGEEDLTVKMSDRGGGVPLRKIDRLFNYMYSTAPRPRVETSRAVPLAGFGYGLPISRLYAQYFQGDLKLYSLEGYGTDAVIYIKALSTESVERLPVYNKAAWKHYKANHEADDWCVPSREPKDMTTFRSS
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

48.7kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 10xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The expression region of this recombinant Mouse Pdk1 covers amino acids 27-434. The theoretical molecular weight of the Pdk1 protein is 48.7 kDa. This Pdk1 recombinant protein is manufactured in baculovirus. The N-terminal 10xHis tag was fused into the coding gene segment of Pdk1, making it easier to detect and purify the Pdk1 recombinant protein in the later stages of expression and purification.

Pyruvate dehydrogenase kinase isoform 1 (Pdk1) is a highly studied protein, with a primary focus on metabolic regulation and the field of tumor biology. As a critical regulator of the tricarboxylic acid cycle, Pdk1 influences cellular energy metabolism by inhibiting decarboxylase activity through phosphorylation. One of its most crucial research directions is its role in tumor metabolism regulation. Pdk1 is found to be overexpressed in various cancers, closely linked to tumor growth and metabolic reprogramming. Additionally, Pdk1 is implicated in multiple research areas, including cardiovascular diseases, as its regulation of metabolic pathways and signaling networks positions it as a potential drug target for the treatment and prevention of related diseases.

Target Background

Function

Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress.

Gene References into Functions

In Agrp Pdk1-/- female mice stature and femur length were shorter. Bone mineral density in the femur decreased, and bone formation was delayed. PMID: 28462077
HIF-1alpha-PDK1-mediated metabolic changes occur in mild hypoxia, where mitochondrial cytochrome c oxidase activity is unimpaired, suggesting a mode of glycolytic reprogramming. PMID: 27189088
The results demonstrate a novel role of PDK1 in AgRP neurons to counteract the high salt diet-induced hypertension by preventing hyperactivation of paraventricular nucleus nesfatin-1 neurons. PMID: 29705701
Here, the authors show that loss of Fxn in the nervous system in mice also activates an iron/sphingolipid/PDK1/Mef2 pathway, indicating that the mechanism is evolutionarily conserved. PMID: 27901468
PDK1 has broad effects in hematopoiesis and is a critical factor for engraftment of both hematopoietic stem cells and multipotent progenitors upon transplantation to recipient mice. PMID: 28182733
PTEN is required for stabilization of planar cell packing in the neural plate and for the formation of stable apical-basal microtubule arrays, while PDPK1 is required for stabilization of apical junctions during epithelial morphogenesis. PMID: 26809587
results thus reveal an essential role for the PDK1-Akt pathway in the regulation of a key step of neuronal migration. PMID: 27170189
PDK1 plays a pivotal role in regulating cardiac function and tumor metastasis by interfering with microenvironment. PMID: 25601552
These findings reveal that there are divergent roles of PDKs during oocyte maturation and indicate a new mechanism controlling meiotic structure. PMID: 25991547
Our data suggest that two arms of the glucose metabolism synergistically regulate the differential activation of macrophages. Our findings also highlight the central role of PDK1 in this event via controlling glycolysis and glucose oxidation. PMID: 25964487
PDHK1 is expressed in Th17 cells, but not Th1 cells, and at low levels in Tregs, and inhibition or knockdown of PDHK1 selectively suppressed Th17 cells and increased Tregs. PMID: 25437876
The tyrosine phosphorylation enhances PDHK1 kinase activity by promoting ATP and PDC binding. PMID: 22195962
Pdk1 enzyme is critical in conserving mitochondrial function by diverting metabolic intermediates to glycolysis PMID: 20160671

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Subcellular Location

Mitochondrion matrix.

Protein Families

PDK/BCKDK protein kinase family

Database Links

KEGG: mmu:228026

STRING: 10090.ENSMUSP00000006669

UniGene: Mm.34411

Code	CSB-BP807321MO
Abbreviation	Recombinant Mouse Pdk1 protein
MSDS	MSDS Datasheet
Size	$528
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Mouse [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial (Pdk1)

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