Product Details

Purity

Greater than 95% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Activity

Measured by its binding ability in a functional ELISA.Immobilized Mouse Ror1 at 2 μg/mL can bind Anti-ROR1 recombinant antibody (CSB-RA020067MA2HU). The EC50 is 0.8551-0.9363 ng/mL.

Target Names

Ror1

Uniprot No.

Q9Z139

Alternative Names

Inactive tyrosine-protein kinase transmembrane receptor ROR1; mROR1; Neurotrophic tyrosine kinase, receptor-related 1; Ror1; Ntrkr1

Species

Mus musculus (Mouse)

Source

Mammalian cell

Expression Region

30-403aa

Target Protein Sequence

QETELSVSAELVPTSSWNTSSEIDKGSYLTLDEPMNNITTSLGQTAELHCKVSGNPPPSIRWFKNDAPVVQEPRRISFRATNYGSRLRIRNLDTTDTGYFQCVATNGKKVVSTTGVLFVKFGPPPTASPGSSDEYEEDGFCQPYRGIACARFIGNRTVYMESLHMQGEIENQITAAFTMIGTSSHLSDKCSQFAIPSLCHYAFPYCDETSSVPKPRDLCRDECEVLENVLCQTEYIFARSNPMILMRLKLPNCEDLPQPESPEAANCIRIGIPMADPINKNHKCYNSTGVDYRGTVSVTKSGRQCQPWNSQYPHTHSFTALRFPELNGGHSYCRNPGNQKEAPWCFTLDENFKSDLCDIPACDSKDSKEKNKME

Mol. Weight

44.6 kDa

Protein Length

Partial

Tag Info

C-terminal 10xHis-tagged

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

ROR1 serves as a promising target in oncology and developmental neuroscience, yet its pseudokinase status and complex extracellular architecture demand native post-translational modifications for faithful ligand recognition. This mammalian-expressed fragment spanning residues 30–403 captures the extracellular domain and satisfies the purity and endotoxin criteria typical for therapeutic antibody screening, with greater than 95% purity by SDS-PAGE and less than 1.0 EU/μg endotoxin. Functional ELISA validation demonstrates specific antibody binding with an EC50 of 0.8551–0.9363 ng/mL when the protein is immobilized at 2 μg/mL, providing quantitative evidence that supports its use in competitive inhibition assays, blocking antibody screening, and epitope mapping studies. The confirmed binding activity makes this protein appropriate for SPR or BLI affinity characterization, small-molecule inhibitor screening, and as a positive control in receptor-ligand interaction platforms targeting ROR1-dependent signaling pathways.

Target Background

Function

Has very low kinase activity in vitro and is unlikely to function as a tyrosine kinase in vivo. Receptor for ligand WNT5A which activate downstream NFkB signaling pathway and may result in the inhibition of WNT3A-mediated signaling. In inner ear, crucial for spiral ganglion neurons to innervate auditory hair cells.

Gene References into Functions

Ror1 has a critical role in regulating SC proliferation during skeletal muscle regeneration PMID: 28790171
Ror1 is crucial for spiral ganglion neurons to innervate auditory hair cells. Impairment of ROR1 function largely affects development of the inner ear and hearing in humans and mice. PMID: 27162350
Although Ror1-mutant mice show no apparent defects in ureteric bud (UB) formation, Ror1; Ror2-double-mutant mice exhibit either defects in UB outgrowth and branching morphogenesis, associated with the loss of the MM from the UB domain, or ectopic formation of the UB. PMID: 26840931
Wnt5a-Ror1 and Wnt5a-Ror2 signaling pathways regulated neurogenesis in the developing neocortex. PMID: 22328498
results identify resistin as a potential inhibitory ligand for the receptor ROR1 and demonstrate, for the first time, that ROR1 plays an important role in adipogenesis and glucose homeostasis in 3T3-L1 cells PMID: 22074948
loss of the orphan receptor Ror1 results in a variety of phenotypic defects within the skeletal and urogenital systems PMID: 20593419
Expression and subcellular localization of Ror tyrosine kinase receptors are developmentally regulated in cultured hippocampal neurons. PMID: 12898527
The ROR1 protein suppression using antisense oligonucleotides or RNA interference (RNAi), neurons extended shorter and less branched minor processes when compared to those in control cells. PMID: 15654020

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Subcellular Location

Membrane; Single-pass type I membrane protein. Cell projection, axon.

Protein Families

Protein kinase superfamily, Tyr protein kinase family, ROR subfamily

Tissue Specificity

At postnatal P0, expressed in heart, lung, liver, kidney, spleen and inner ear.

Database Links

KEGG: mmu:26563

STRING: 10090.ENSMUSP00000048171

UniGene: Mm.485829

Code	CSB-MP4008MO
Abbreviation	Recombinant Mouse Ror1 protein, partial (Active)
MSDS	MSDS Datasheet
Size	$136
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Activity Measured by its binding ability in a functional ELISA.Immobilized Mouse Ror1 at 2 μg/ml can bind Anti-ROR1 recombinant antibody (CSB-RA020067MA2HU). The EC₅₀ is 0.8551-0.9363 ng/mL. Biological Activity Assay
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Recombinant Mouse Inactive tyrosine-protein kinase transmembrane receptor ROR1 (Ror1), partial (Active)

Product Details

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Target Background

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