ROR1, or Receptor Tyrosine Kinase-like Orphan Receptor 1, also known as Neurotrophic Tyrosine Kinase, Receptor-related 1, is a Type I transmembrane protein and a member of the Receptor Tyrosine Kinases (RTKs) family. ROR1 is either lowly expressed or not expressed in normal human tissues but is highly expressed in a variety of malignant tumors or tissues, such as Chronic Lymphocytic Leukemia (CLL), breast cancer, ovarian cancer, melanoma, lung adenocarcinoma, and others. The mechanism of action of ROR1 involves mediating the non-canonical Wnt signaling pathway, particularly Wnt5a, participating in the phosphorylation of the NF-κB subunit p65, activating the NF-κB pathway in tumor cells, and promoting cell migration and invasion. As a highly specific tumor-associated antigen, ROR1 has become a potentially attractive target in cancer therapy.
Validated Data
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Activity
Measured by its binding ability in a functional ELISA. Immobilized ROR1 at 2 μg/ml can bind anti-ROR1 antibody(CSB-RA020067A1HU), the EC50 is 0.2450-0.3416 ng/mL.
The purity of ROR1 was greater than 90% as determined by SEC-HPLC
ROR1 Recombinant Monoclonal Antibody (CSB-RA020067A1HU)
Validated Data
The Binding Activity of ROR1 with Anti-ROR1 recombinant antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human ROR1(CSB-MP020067HU1d7) at 2 μg/mL can bind Anti-ROR1 recombinant antibody, the EC50 is 0.2450-0.3416 ng/mL.
Untransfected CT26 cells (green line) and transfected Human ROR1 CT26 stable cells (red line) were stained with anti-ROR1 antibody (2µg/1*106cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.
The following ROR1 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
ROR1 Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA020067LC01HU | ROR1 Antibody, FITC conjugated | Human | |
CSB-PA020067LA01HU | ROR1 Antibody | Human | ELISA, IHC |
CSB-PA020067GA01HU | ROR1 Antibody | Human,Mouse,Rat | ELISA,WB |
CSB-PA067964 | ROR1 Antibody | Human,Mouse | ELISA,IHC |
CSB-PA993257 | ROR1 Antibody | Human,Mouse | ELISA,IHC |
CSB-RA020067A1HU | ROR1 Recombinant Monoclonal Antibody | Human | ELISA, FC |
ROR1 Proteins for Homo sapiens (Human)
Code | Product Name | Source |
---|---|---|
CSB-YP020067HU | Recombinant Human Tyrosine-protein kinase transmembrane receptor ROR1 (ROR1), partial | Yeast |
CSB-EP020067HU | Recombinant Human Tyrosine-protein kinase transmembrane receptor ROR1 (ROR1), partial | E.coli |
CSB-MP020067HU CSB-EP020067HU-B |
Recombinant Human Tyrosine-protein kinase transmembrane receptor ROR1 (ROR1) | Mammalian cell In Vivo Biotinylation in E.coli |
CSB-MP020067HU1d7 | Recombinant Human Inactive tyrosine-protein kinase transmembrane receptor ROR1 (ROR1), partial (Active) | Mammalian cell |