Purity
Greater than 90% as determined by SDS-PAGE.
Alternative Names
IL5; Interleukin-5; IL-5; Eosinophil differentiation factor; T-cell replacing factor; TRF
Expression Region
22-134aa
Target Protein Sequence
VENTMNRLVAETLTLLSIHRTLLIGDGNLMISTPVHTNHQLCIEEVFQGIDTLKNQTARGDAVEKLFQNLSLIKEYIDRQKKNCGGERWRVTQFLDYLQVFLGVINTEWTMES
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The expression region of this recombinant Pig IL5 covers amino acids 22-134. The theoretical molecular weight of the IL5 protein is 17 kDa. This IL5 protein is produced using e.coli expression system. The N-terminal 6xHis tag was fused into the coding gene segment of IL5, making it easier to detect and purify the IL5 recombinant protein in the later stages of expression and purification.
IL5, a member of the interleukin family, is a cytokine that plays a crucial role in the immune system. IL5 is primarily produced by T-helper 2 (Th2) cells and is involved in the regulation of immune responses, particularly those related to eosinophils. This cytokine promotes the growth, activation, and differentiation of eosinophils, which are white blood cells involved in the immune response against parasites and in allergic reactions. IL5 is essential for maintaining the appropriate balance of immune cells and is implicated in various inflammatory and allergic conditions. Studying pig IL5 contributes to understanding immune regulation in pigs and may have implications for veterinary medicine and livestock health.
