Recombinant Rat Guanine nucleotide-binding protein G (k) subunit alpha (Gnai3)

Code CSB-YP009591RA
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Source Yeast
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Code CSB-EP009591RA
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Source E.coli
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Code CSB-EP009591RA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP009591RA
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Source Baculovirus
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Code CSB-MP009591RA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Gnai3
Uniprot No.
Alternative Names
Gnai3; Gnai-3Guanine nucleotide-binding protein G(i) subunit alpha; G(i) alpha-3
Species
Rattus norvegicus (Rat)
Expression Region
2-354
Target Protein Sequence
GCTLSAEDK AAVERSKMID RNLREDGEKA AKEVKLLLLG AGESGKSTIV KQMKIIHEDG YSEDECKQYK VVVYSNTIQS IIAIIRAMGR LKIDFGEAAR ADDARQLFVL AGSAEEGVMT SELAGVIKRL WRDGGVQACF SRSREYQLND SASYYLNDLD RISQTNYIPT QQDVLRTRVK TTGIVETHFT FKELYFKMFD VGGQRSERKK WIHCFEGVTA IIFCVALSDY DLVLAEDEEM NRMHESMKLF DSICNNKWFT DTSIILFLNK KDLFEEKIKR SPLTICYPEY TGSNTYEEAA AYIQCQFEDL NRRKDTKEVY THFTCATDTK NVQFVFDAVT DVIIKNNLKE CGLY
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Heterotrimeric guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades. The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state. Signaling by an activated GPCR promotes GDP release and GTP binding. The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal. Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins. Signaling is mediated via effector proteins, such as adenylate cyclase. Inhibits adenylate cyclase activity, leading to decreased intracellular cAMP levels. Stimulates the activity of receptor-regulated K(+) channels. The active GTP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division.
Gene References into Functions
  1. Nitric oxide decreased the enhanced expression of Gialpha-2/3 proteins and hyperproliferation of Vascular smooth muscle cells from spontaneously hypertensive rats by cGMP-independent mechanism. PMID: 28692698
  2. These results indicate that NPR-C activation by C-ANP4-23 attenuates the enhanced levels of cell cycle proteins through the inhibition of enhanced expression of Gialpha proteins. PMID: 24155894
  3. Results suggest that the suppression of Dopamine-2 (D2) receptor short (D2S)-regulated Gi3 inhibition of Gs protein may be one of the mechanisms controlling estradiol (E(2)-activated prolactin (PRL) synthesis and cell proliferation in lactotropes. PMID: 22573829
  4. studies suggest a possible role for zymogen granule-associated G(alpha)(i3) in zymogen granule-plasma membrane fusion PMID: 12038977
  5. Suggest role for oxidative stress in high glucose-induced decreased expression of Gnai2/3 and adenylyl cyclase signaling in vascular smooth muscle cells. PMID: 18441196

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Subcellular Location
Cytoplasm. Cell membrane; Lipid-anchor. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
Protein Families
G-alpha family, G(i/o/t/z) subfamily
Tissue Specificity
Ubiquitous.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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