Product Details

Purity

≥85% as determined by SDS-PAGE.

Activity

Not Test

Target Names

Igfbp3

Uniprot No.

P15473

Research Area

Signal Transduction

Alternative Names

IBP-3; IGF-binding protein 3; IGFBP-3

Species

Rattus norvegicus (Rat)

Source

Yeast

Expression Region

28-292aa

Target Protein Sequence

GAGAVGAGPVVRCEPCDARALAQCAPPPTAPACTELVREPGCGCCLTCALREGDACGVYTERCGTGLRCQPRPAEQYPLKALLNGRGFCANASAASNLSAYLPSQPSPGNTTESEEDHNAGSVESQVVPSTHRVTDSKFHPLHSKMEVIIKGQARDSQRYKVDYESQSTDTQNFSSESKRETEYGPCRREMEDTLNHLKFLNVLSPRGVHIPNCDKKGFYKKKQCRPSKGRKRGFCWCVDKYGQPLPGYDTKGKDDVHCLSVQSQ
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

30.4 kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Datasheet & COA

Please contact us to get it.

Target Background

Function

IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R. Promotes testicular germ cell apoptosis.

Gene References into Functions

Compound 49b treatment inhibited these retinal changes. In REC cultured in hypoxia, levels of IGFBP-3 were reduced, which were significantly increased by Compound 49b. PMID: 27439004
Study indicated that IGFBP-3 shRNA might rehabilitate erectile function via decreased concentrations of serum LDL lipoprotein and triglyceride, increased cavernosal smooth muscle and improvement in NO-cGMP signaling in diabetic rats. PMID: 25879169
IGFBP-3 shRNA may rehabilitate erectile function via increases of concentrations of serum testosterone and cavernous cyclic guanosine monophosphate in streptozotocin-induced diabetic rats PMID: 25582342
IGFBP-3 has novel protective effects on retinal and systemic vasculature and may be a therapeutic candidate for ocular complications such as diabetic retinopathy. PMID: 22792172
obesity induces specific changes in the expression profile of the adipose tissue depot surrounding the pancreas, leading to a decrease in IGFBP3 secretion PMID: 22067319
The results support the suggestion that inactivation of Mxi1 has a positive effect on cell proliferation by down-regulating IGFBP-3. PMID: 22008548
Paraquat-treated rats showed enhanced gene expression of growth factors such as platelet-derived growth factor-A and insulin-like growth factor-1 at 21 days and transforming growth factor-beta 1 at 14 days. PMID: 20498031
Data suggest that the inhibitory effect of chronic arthritis on skeletal muscle is higher in fast glycolytic than in slow oxidative muscle and that IGF-I administration attenuates this effect and decreases atrogin-1 and IGFBP-3 gene expression. PMID: 20519361
Downregulation of IGFBP-3 gene expression may cause pulmonary hypoplasia in the nitrofen-induced CDH model by interfering with retinoid signaling pathway. PMID: 19844724
IGFBP-3 and BAX interaction activates germ cell apoptosis via the mitochondria-dependent pathway. PMID: 19887447
IGFBP-3 and -5 proteins may have potential modulatory roles in type I fiber-dominated muscles, aging, and regrowth from atrophy. PMID: 12397024
role of glucocorticoids in the insulin-like growth factor binding protein (IGFBP)-3 response to endotoxin PMID: 12485840
Expressed in cardiac muscle cells. Serum withdrqwal-induced increase in apoptosis of cardiac muscle cells associated with IGFBP-3. PMID: 12508914
data indicate that acute inflammation inhibits serum concentrations of IGFBP-3 by decreasing its synthesis in the liver, rather than increasing its proteolysis PMID: 12581876
Aminoguanidine inhibition of inducible nitric oxide synthase blocked the inhibitory effect of LPS on both IGFBP-3 serum levels and its hepatic mRNA levels. PMID: 13129855
IGFBP-3 could play proapoptotic action at the myocardial level and suggest a novel role for this protein in cardiovascular dysfunction. PMID: 15055478
IGF-I uses different and only partially overlapping intracellular signaling pathways for regulation of IGFBP-3 and -5 with opposing biological functions might be important for modulation of IGF bioactivity in cellular microenvironment. PMID: 15845624
cross-talk between the IGFBP-3-dependent STAT-1 signaling and the TGF-beta-dependent ERK pathway that regulates mesenchymal chondroprogenitor cell proliferation PMID: 15956343
In utero dibutyl phthalate leads to IGFBP3 expression in rat testis in fetal and postnatal development. PMID: 16037377
retinoic acid modulates the secreted IGFBP-2 and -3 during O(2) exposure and inhibits the increase in collagen that occurs during lung injury PMID: 16055936
Liver cirrhosis is accompanied by an altered spatial expression of IGFBP-3 PMID: 16098781
Intracerebroventricular infusion of IGFBP-3 significantly impaired insulin action at the liver (45% increase in hepatic glucose production, P < 0.01). PMID: 17003344

Hide All

Subcellular Location

Secreted.

Database Links

KEGG: rno:24484

STRING: 10116.ENSRNOP00000011678

UniGene: Rn.26369

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：
Please enter the characters shown. Click the image to refresh if unclear.

Code	CSB-YP011097RA
Abbreviation	Recombinant Rat Igfbp3 protein
MSDS	MSDS Datasheet
Size	$306
	Order now
Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Have Questions?	Leave a Message or Start an on-line Chat

Recombinant Rat Insulin-like growth factor-binding protein 3 (Igfbp3)

Product Details

Related Products

Customer Reviews and Q&A

Target Background

×CloseMessage