Recombinant Rat Proliferating cell nuclear antigen(Pcna)

Code CSB-YP017621RA
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Source Yeast
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Code CSB-EP017621RA
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Source E.coli
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Code CSB-EP017621RA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP017621RA
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Source Baculovirus
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Code CSB-MP017621RA
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Pcna
Uniprot No. P04961
Alternative Names Pcna; Proliferating cell nuclear antigen; PCNA; Cyclin
Species Rattus norvegicus (Rat)
Expression Region 1-261
Target Protein Sequence MFEARLIQGS ILKKVLEALK DLINEACWDI SSGGVNLQSM DSSHVSLVQL TLRSEGFDTY RCDRNLAMGV NLTSMSKILK CAGNEDIITL RAEDNADTLA LVFEAPNQEK VSDYEMKLMD LDVEQLGIPE QEYSCVVKMP SGEFARICRD LSHIGDAVVI SCAKDGVKFS ASGELGNGNI KLSQTSNVDK EEEAVSIEMN EPVQLTFALR YLNFFTKATP LSPTVTLSMS ADVPLVVEYK IADMGHLKYY LAPKIEDEEG S
Protein Length Full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Data

Function Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair
Gene References into Functions
  1. The damaged retinal pigment epithelium showed earlier expression of PCNA and RPE65 than photoreceptor out segments PMID: 29569173
  2. Taken together, these results indicated that ALA protected dopaminergic neurons against MPP+induced neurotoxicity through its ability to upregulate the DNA repair protein, PCNA, via the P53 pathway. PMID: 27665784
  3. This indicates that MPP+-induced oxidative damage is mediated by the downregulation of PCNA through the p53 pathway in a cellular model of Parkinson's disease . PMID: 26677001
  4. PCNA index decreased from 80 in HCC rats to 32 after boron treatment. PMID: 23219601
  5. The positive expression of PCNA in intestinal epithelial cells at the early development stage was much stronger and more extensive than that in mature rats. Expression was limited to intestinal crypts. PMID: 12857463
  6. Islet cell tumors were associated with increased nuclear expression of cyclin-dependent kinase 4 as well as increased proliferating cell nuclear antigen and decreased beta-catenin expression. PMID: 22477723
  7. Fluorine possibly damages the male reproductive system by reducing the expression of TERT and PCNA. PMID: 17456401
  8. Sex-associated difference in PCNA expression in N-methyl-N'-nitro-N-nitrosoguanidine-induced gastric cancers in rats. PMID: 22330348
  9. Glutamine supplementation rapidly improved the expression of PCNA after cisplatin-induced intestinal mucosal injury. PMID: 21080177
  10. Following melatonin treatment, pinealectomized female rats present signals of proliferation on ovarian surface epithelium and interstitial cells as well as high expressions of ovarian PCNA and vascular endothelial growth factor. PMID: 20605140
  11. High omega-6 polyunsaturated fatty acid diet can enhance azoxymethane-induced inhibition of P53 in colon mucosa, resulting in overexpression of PCNA, formation of aberrant crypt foci, and carcinogenesis in the colon. PMID: 20972911
  12. After treatment with Buyang Huanwu Decoction PCNA level was increased in rats with aortic intimal injuries. PMID: 18664354
  13. Compared with the control group, the PCNA level in the thyroid of rats given propylthiouracil for 3 days significantly increased. PMID: 18390212
  14. Exposure to diesel exhaust particles elevated transcription of proliferating cell nuclear antigen gene in rat alveolar macrophages. PMID: 12011483
  15. The presence of PCNA in a key anatomical location pertinent to an evolving ischaemic lesion indicates that PCNA influences cell survival in ischaemically compromised tissue. PMID: 12099899
  16. PCNA expression gradually decreased from prenatal day 19 onwards and has a role in growth regulation in the developing rat heart PMID: 12389741
  17. data show that changes in liver expression of DNA polymerase beta, Ref-1, proliferating cell nuclear antigen, and Bax are associated with peroxisome proliferator-induced carcinogenesis PMID: 12565796
  18. Activation of vascular smooth muscle cell Ca2+ channels and CaMKII is a key early signaling event required for upregulation of PCNA gene expression in VSMCs after oxidative injury to endothelium. PMID: 12969989
  19. ERK1/2 has a role in FSH induced PCNA expression and steroidogenesis in granulosa cells PMID: 15569628
  20. Examination by immunofluorescence techniques of tracheal cell cultures after exposure to very low doses of alpha particles revealed a large proportion of cells with proliferating cell nuclear antigen (PCNA) bound in their nuclei. PMID: 15606305
  21. PCNA-positive cell level was markedly higher in cadmium intoxication in submandibular gland. PMID: 15638412
  22. PCNA may play an important role in the damage and repair of intestinal mucosa in intrauterine ischemia PMID: 15818741
  23. regulation of PCNA through YY1 is affected by nucleophospmin/B23 PMID: 16099430
  24. In HCC, there was positive correlation between p-Stat3 level and the expression of TGFalpha and PCNA. PMID: 17031671
  25. PCNA may play a role in abnormal placenta formation resulting from diabetes PMID: 18377963
  26. Ovarian follicle counts using Pcna and semi-automated image analysis is reported. PMID: 18467674
  27. Pcna indexing as a preclinical immunohistochemical biological marker for testicular toxicity is reported. PMID: 19016366
  28. Proliferating cell nuclear antigen shRNA treatment attenuates chronic proliferative cholangitis in rats. PMID: 19032457

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Subcellular Location Nucleus
Protein Families PCNA family
Database Links

KEGG: rno:25737

STRING: 10116.ENSRNOP00000028887

UniGene: Rn.223

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