Product Details

Purity

>85% (SDS-PAGE)

Target Names

Rps6kb1

Uniprot No.

P67999

Alternative Names

Rps6kb1Ribosomal protein S6 kinase beta-1; S6K-beta-1; S6K1; EC 2.7.11.1; 70 kDa ribosomal protein S6 kinase 1; P70S6K1; p70-S6K 1; Ribosomal protein S6 kinase I; p70 ribosomal S6 kinase alpha; p70 S6 kinase alpha; p70 S6K-alpha; p70 S6KA

Species

Rattus norvegicus (Rat)

Expression Region

1-525

Target Protein Sequence

MRRRRRRDGF YPAPDFRHRE AEDMAGVFDI DLDQPEDAGS EDELEEGGQL NESMDHGGVG PYELGMEHCE KFEISETSVN RGPEKIRPEC FELLRVLGKG GYGKVFQVRK VTGANTGKIF AMKVLKKAMI VRNAKDTAHT KAERNILEEV KHPFIVDLIY AFQTGGKLYL ILEYLSGGEL FMQLEREGIF MEDTACFYLA EISMALGHLH QKGIIYRDLK PENIMLNHQG HVKLTDFGLC KESIHDGTVT HTFCGTIEYM APEILMRSGH NRAVDWWSLG ALMYDMLTGA PPFTGENRKK TIDKILKCKL NLPPYLTQEA RDLLKKLLKR NAASRLGAGP GDAGEVQAHP FFRHINWEEL LARKVEPPFK PLLQSEEDVS QFDSKFTRQT PVDSPDDSTL SESANQVFLG FTYVAPSVLE SVKEKFSFEP KIRSPRRFIG SPRTPVSPVK FSPGDFWGRG ASASTANPQT PVEYPMETSG IEQMDVTTSG EASAPLPIRQ PNSGPYKKQA FPMISKRPEH LRMNL

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Serine/threonine-protein kinase that acts downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression. Regulates protein synthesis through phosphorylation of EIF4B, RPS6 and EEF2K, and contributes to cell survival by repressing the pro-apoptotic function of BAD. Under conditions of nutrient depletion, the inactive form associates with the EIF3 translation initiation complex. Upon mitogenic stimulation, phosphorylation by the mammalian target of rapamycin complex 1 (mTORC1) leads to dissociation from the EIF3 complex and activation. The active form then phosphorylates and activates several substrates in the pre-initiation complex, including the EIF2B complex and the cap-binding complex component EIF4B. Also controls translation initiation by phosphorylating a negative regulator of EIF4A, PDCD4, targeting it for ubiquitination and subsequent proteolysis. Promotes initiation of the pioneer round of protein synthesis by phosphorylating POLDIP3/SKAR. In response to IGF1, activates translation elongation by phosphorylating EEF2 kinase (EEF2K), which leads to its inhibition and thus activation of EEF2. Also plays a role in feedback regulation of mTORC2 by mTORC1 by phosphorylating RICTOR, resulting in the inhibition of mTORC2 and AKT1 signaling. Mediates cell survival by phosphorylating the pro-apoptotic protein BAD and suppressing its pro-apoptotic function. Phosphorylates mitochondrial RMP leading to dissociation of a RMP:PPP1CC complex. The free mitochondrial PPP1CC can then dephosphorylate RPS6KB1 at Thr-412, which is proposed to be a negative feedback mechanism for the RPS6KB1 anti-apoptotic function. Mediates TNF-alpha-induced insulin resistance by phosphorylating IRS1 at multiple serine residues, resulting in accelerated degradation of IRS1. In cells lacking functional TSC1-2 complex, constitutively phosphorylates and inhibits GSK3B. May be involved in cytoskeletal rearrangement through binding to neurabin. Phosphorylates and activates the pyrimidine biosynthesis enzyme CAD, downstream of MTOR. Following activation by mTORC1, phosphorylates EPRS and thereby plays a key role in fatty acid uptake by adipocytes and also most probably in interferon-gamma-induced translation inhibition.

Gene References into Functions

This study observed that high-intensity interval training increased the hippocampal and cortical levels of phosphorylated P70S6K in comparison to continuous endurance training. PMID: 28898133
Cdk5 phosphorylates p70 Ribosomal S6 Kinase 1 at Ser-411 in neuron, and loss of this phosphorylation event reduces the density of dendritic spines. PMID: 25903132
Ribosomal protein S6 kinase 1 signaling in prefrontal cortex has a role in controlling depressive behavior in a rat model PMID: 25918363
protein levels of activated S6 kinase 1 were significantly reduced following treatment with everolimus PMID: 25200811
Data indicate that activation of RNA polymerase II and p70 S6 kinase (P70S6K) are increased in apoptotic neurons, and their inhibition prevents apoptosis. PMID: 25568312
Data suggest that, in skeletal muscle, mTORC1 (mechanistic target of rapamycin, complex 1)/JNK (mitogen-activated protein kinase 8) signaling coordinates site-specific phosphorylation of p70S6K1; mTORC1/mTORC2 play roles in inhibition of JNK (Mapk8). PMID: 24801387
in adipose tissue phosphorylated Threonine in S6K1 is a marker for increased glucose levels PMID: 22705322
Insulin stimulation of SREBP-1c processing in transgenic rat hepatocytes requires p70 S6-kinase. PMID: 22927400
Data show that hydrogen sulfide acts via cAMP-mediated PI3K/Akt/p70S6K signal pathways to inhibit hippocampal neuronal apoptosis and protect neurons from oxygen glucose deprivation/reoxygenation injury. PMID: 20967511
Ingestion of a higher quality and quantity of dietary protein stimulates the phosphorylation of 4E-BP1 and S6K1 in the brain and increases the brain protein synthesis in the aged rats. PMID: 21228503
Data show that weak, sustained EGFR phosphorylation, rather than strong, transient phosphorylation, strongly induced phosphorylation of the ribosomal protein S6, a molecule downstream of Akt. PMID: 20664065
Collectively, these results indicate that the basal level of intracellular calcium gates BDNF-induced activation of p70S6K and protein synthesis through CaM. PMID: 20029971
glucose-induced p70(S6Kinase) activation in pancreatic beta-cells was mediated by a distinct signaling pathway independent of Calcium(2+)/cAMP PMID: 12663469
p70S6K plays a key role in retinal neuronal cell survival PMID: 14660591
Phosphorylation of S6K1 is controlled by the Tsc1-Tsc2 complex. PMID: 14993219
L-Arginine regulates p70 S6 kinase activity and phosphorylation of 4E-BP1 through mTOR signaling pathway PMID: 15010853
Follicle-stimulating hormone -induced phosphorylation of the S6 ribosomal protein, a cellular substrate of p70S6K, requires PKA activity. PMID: 15774499
Results indicate marked alterations in the p70S6k signaling pathway with aging. PMID: 16087221
CDK-mediated phosphorylation regulates the activation of S6K1. PMID: 17220300
AMPK activation attenuates S6K1 response to high-frequency electrically stimulated skeletal muscle contractions. PMID: 18187610
The decrease in S6K-1 expression might be an important factor affecting either glucose- or leucine-induced insulin secretion. PMID: 18234235
Activation of hypothalamic Rps6kb1 mediates diet-induced hepatic insulin resistance. PMID: 18618016
Mediobasal hypothalamic ribosomal protein S6 kinases activity bidirectionally drives behavioral and metabolic determinants of energy balance. PMID: 19041762

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Subcellular Location

Cytoplasm. Cell junction, synapse, synaptosome. Mitochondrion outer membrane. Mitochondrion.

Protein Families

Protein kinase superfamily, AGC Ser/Thr protein kinase family, S6 kinase subfamily

Tissue Specificity

Brain.

Database Links

KEGG: rno:83840

STRING: 10116.ENSRNOP00000005226

UniGene: Rn.4042

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Recombinant Rat Ribosomal protein S6 kinase beta-1 (Rps6kb1)

Product Details

Customer Reviews and Q&A

Target Background

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