Recombinant Xenopus laevis Serine/threonine-protein kinase Chk1 (chek1)

Code CSB-YP738530XBE
MSDS
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Source Yeast
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Code CSB-EP738530XBE
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Source E.coli
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Code CSB-EP738530XBE-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP738530XBE
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Source Baculovirus
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Code CSB-MP738530XBE
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
chek1
Uniprot No.
Alternative Names
chek1; chk1Serine/threonine-protein kinase Chk1; EC 2.7.11.1; CHK1 checkpoint homolog; Checkpoint kinase-1; xChk1
Species
Xenopus laevis (African clawed frog)
Expression Region
1-474
Target Protein Sequence
MAVPFVEDWD LVQTLGEGAY GEVQLAVNRK TEEAVAVKIV DMTRAADCPE NIKKEICINR MLSHTNIVRF YGHRREGNIQ YLFLEYCRGG ELFDRIEPDV GMPEQDAQKF FQQLIAGVEY LHSIGITHRD IKPENLLLDE RDQLKISDFG LATVFRHNGK ERLLNKMCGT LPYVAPELIK SRAFHADPVD VWSCGIVLTA MLAGELPWDQ PNEVCQEYCD WKEKNHYLTP WKKISATPLA LLCKMLTENP QSRITIPDIK KDRWFTEIIK KGLKRSRVIS GGSSDSSVLC KQIRSDIDIS HFSHSEEKTA LSSTQPEPRT ALATWDSNSS YIDNLVQGKG ISFSQPACPD NMLLNSQLIG TPGSSQNVWQ RLVKRMTRFF TKVNAESSYS NLMDTCEKMG YVLKKSCANE VTLSTTDRRN NKLIFKVNLV EMEDRILLDF RLSKGDGLEF KRHFLKIKKK MDAVVAVQKV LPDT
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. This regulation is achieved by a number of mechanisms that together help to preserve the integrity of the genome. Recognizes the substrate consensus sequence [R-X-X-S/T]. Phosphorylates wee1 at 'Ser-549' and cdc25c at 'Ser-287', which creates binding sites for 14-3-3 proteins which activate wee1 and inhibit cdc25c. Phosphorylates cdc25a at 'Ser-504' which prevents the interaction of cdc25a with CDK2-cyclin E1, CDC2-cyclin A1 and CDC2-cyclin B1. This inhibitory effect does not require 14-3-3 protein binding. Activation of wee1 and inhibition of CDC25 results in increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and consequent inhibition of cell cycle progression. May promote DNA repair, regulate chromatin assembly and the transcription of genes that regulate cell-cycle progression. May also play a role in replication fork maintenance.
Gene References into Functions
  1. Inhibition of Drf1 is the primary mechanism by which Chk1 blocks cell-cycle progression in the early embryo and is an essential function of Chk1 at the blastula-to-gastrula stage of development. PMID: 28697335
  2. The study analyzes the role of Chk1 in the replication program in sperm nuclei replicating in Xenopus egg extracts by a combination of experimental and modelling approaches. PMID: 26046346
  3. ATR-Chk1 DDR pathway appears to be dispensable for the preferential association of REV1 to MMC-damaged chromatin. PMID: 25800873
  4. Results show that Chk1 negatively regulates the Treslin-mediated loading of Cdc45 onto chromatin and thereby serves to antagonize the initiation of replication. PMID: 25557548
  5. DNA polymerase kappa-dependent DNA synthesis at stalled replication forks is important for CHK1 activation. PMID: 23799366
  6. APE2 associates with Chk1; a serine residue (S86) in the Chk1-binding motif of APE2 is essential for Chk1 phosphorylation, indicating a Claspin-like but distinct role for APE2 in ATR-Chk1 signaling. PMID: 23754435
  7. Findings reveal that XH2AX has a specific role in anterior neural formation of Xenopus, which is mediated through phosphorylation of XH2AX at Thr(16) by Chk1. PMID: 20639511
  8. mechanism of Chk1 activation at the DNA replication checkpoint PMID: 14767054
  9. the Chk1 pathway involves claspin, which functions in checkpoint control [review] PMID: 15279790
  10. ATRIP must associate with ATR in order for ATR to carry out the phosphorylation of Chk1 effectively PMID: 16186122
  11. Cut5 plays a crucial role in the initial amplification step of the ATR-Chk1 signaling pathway at the stalled replication fork. PMID: 16923121
  12. Study shows that although ssDNA is not sufficient for checkpoint activation, the amount of ssDNA adjacent to the primer influences the level of Chk1 phosphorylation. PMID: 17437996
  13. Chk1 is activated at the midblastula transition in Xenopus laevis embryos independently of DNA content and the cyclin E/Cdk2 developmental timer. PMID: 18414041

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Subcellular Location
Nucleus. Chromosome. Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
Protein Families
Protein kinase superfamily, CAMK Ser/Thr protein kinase family, NIM1 subfamily
Database Links

KEGG: xla:398191

UniGene: Xl.406

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