ABAT Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA242969A0HU
Size	US$210
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Image	IHC image of CSB-RA242969A0HU diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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Target Background

Product Details

Uniprot No.

P80404

Target Names

ABAT

Alternative Names

4-aminobutyrate aminotransferase, mitochondrial (EC 2.6.1.19) ((S)-3-amino-2-methylpropionate transaminase) (EC 2.6.1.22) (GABA aminotransferase) (GABA-AT) (Gamma-amino-N-butyrate transaminase) (GABA transaminase) (GABA-T) (L-AIBAT), ABAT, GABAT

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human ABAT

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

5B6

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

ABAT, or 4-aminobutyrate aminotransferase, serves as a critical mitochondrial enzyme responsible for catabolizing gamma-aminobutyric acid (GABA), the primary inhibitory neurotransmitter in the central nervous system. By regulating GABA availability, ABAT influences neuronal excitability and has emerged as a significant target in neuroscience research, particularly in studies examining seizure disorders, neurological development, and GABAergic signaling pathways. Beyond its neurological roles, ABAT has garnered attention in cancer metabolism research, where altered GABA metabolism may contribute to tumor microenvironment modulation and cellular proliferation.

This recombinant monoclonal antibody, clone 5B6, offers researchers the reproducibility essential for longitudinal studies and multi-site collaborations. Developed using recombinant technology with a defined sequence, this antibody eliminates the lot-to-lot variability inherent in traditional hybridoma-derived reagents, ensuring consistent performance across experiments. The rabbit host origin provides high-affinity binding characteristics, while affinity chromatography purification delivers a clean preparation suitable for demanding applications.

Validation in immunohistochemistry demonstrates reliable detection in paraffin-embedded human liver tissue, where ABAT expression reflects the organ's role in amino acid metabolism. Using a citrate buffer antigen retrieval protocol at pH 6.0 with recommended dilutions of 1:50 to 1:200, researchers can expect clear, specific staining patterns visualized through standard HRP-DAB detection systems. The antibody is also validated for ELISA applications, providing flexibility for both tissue-based localization studies and quantitative protein measurement workflows.

This antibody supports investigations spanning neuroscience, cancer biology, metabolic disorders, and signal transduction research, making it a versatile tool for researchers exploring GABA metabolism and its broader physiological implications.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Catalyzes the conversion of gamma-aminobutyrate and L-beta-aminoisobutyrate to succinate semialdehyde and methylmalonate semialdehyde, respectively. Can also convert delta-aminovalerate and beta-alanine.

Gene References into Functions

This study utilized ER+ IBC to identify a metagene including ABAT and STC2 as predictive biomarkers for endocrine therapy resistance. PMID: 25771305
A-to-G transition at nucleotide 754 of the human ABAT gene identified in lymphoblast cDNA (c.754A>G) results in substitution of an invariant arginine at amino acid 220 by lysine (p.Arg220Lys). This point mutation results in destabilization of the binding of pyridoxal-5'-phosphate to GABA-transaminase (required for transamination of GABA to succinic semialdehyde) and thus results in GABA-transaminase deficiency. PMID: 25485164
Findings point to a possible role of ABAT gene-regulated GABA catabolism for an altered processing of somatosensory stimuli as a potential vulnerability marker for affective disorders. PMID: 22225676
direct involvement of ABAT in pathways affecting lower esophageal sphincter (LES) control in gastro esophageal reflux disease PMID: 21552517
Observational study and genome-wide association study of gene-disease association. (HuGE Navigator) PMID: 20659789
excessive prenatal GABA exposure in the central nervous system (CNS) is responsible for the clinical manifestations of GABA transaminase deficiency [case report] PMID: 20052547
Marked differences in platelet uptake of GABA and activity of catabolic enzyme GABA-T between patients with generalized and localization-related epileptic syndromes. May indicate an impairment in function of brain GABAergic systems. PMID: 12694932
results suggest that the Cys321 residue is essential for the catalytic function of GABAT, and that it is involved in the formation of a disulfide link between two monomers of human brain GABAT PMID: 15528998
lysine 357 is essential for catalytic function of brain GABA transaminase, and is involved in binding PLP at the active site PMID: 15650327
Analysis of the autistic disorder susceptibility locus suggests an association on chromosome 16p between GRIN2A and ABAT. PMID: 15830322

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Involvement in disease

GABA transaminase deficiency (GABATD)

Subcellular Location

Mitochondrion matrix.

Protein Families

Class-III pyridoxal-phosphate-dependent aminotransferase family

Tissue Specificity

Liver > pancreas > brain > kidney > heart > placenta.

Database Links

HGNC: 23

OMIM: 137150

KEGG: hsa:18

STRING: 9606.ENSP00000268251

UniGene: Hs.336768

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Service

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Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Target Therapy

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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