Product Details

Uniprot No.

P30542

Target Names

ADORA1

Alternative Names

ADORA1; Adenosine receptor A1

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human ADORA1

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

10D12

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The detection of human ADORA1 protein in ELISA, WB, and IHC applications can be effectively carried out using the ADORA1 recombinant monoclonal antibody, which is produced using recombinant DNA technology. The hybridomas producing the ADORA1 antibody are formed by fusing B cells isolated from an animal immunized with a synthesized peptide derived from human ADORA1 with myeloma cells. The cDNA of the ADORA1 monoclonal antibody is sequenced, and the gene coding for the ADORA1 antibody is synthesized. The synthesized gene is then cloned into a vector and transfected into cells for cultivation. Finally, the ADORA1 recombinant monoclonal antibody is purified using affinity chromatography from the cell culture supernatant.

The ADORA1 protein is a G protein-coupled receptor that is expressed on the cell surface of many types of cells. In the central nervous system, activation of the ADORA1 protein can inhibit the release of several neurotransmitters, including dopamine, acetylcholine, and glutamate. It is also involved in the modulation of pain perception, reduction of inflammation, as well as regulation of cardiovascular function and glucose metabolism.

Target Background

Function

Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase.

Gene References into Functions

ADORA1 is not a common risk factor or causal gene for Parkinson's disease or dementia with Lewy bodies in the European population PMID: 27987235
monocyte-derived macrophages from ankylosing spondylitis patients expressed increased levels of A2AAR and reduced levels of A1 and A2BAR compared to healthy controls PMID: 29524036
studies revealed individual trait characteristics for being either vulnerable or resilient to both alcohol and to sleep deprivation. Both interventions induce gradual increases in cerebral A1AR availability, pointing to a potential common molecular response mechanism. PMID: 30012607
Data suggested that adenosine A1 receptor might potentiate glycinergic transmission through Galphai/PKA/alpha3 and Gbetagamma/alpha1ins pathways in inflamed rat. PMID: 28882563
Elevated A1 adenosine receptor is associated with sleep deprivation. PMID: 28373571
Mutation in ADORA1 may be associated with early-onset parkinsonism and cognitive dysfunction. PMID: 27134041
Inhibition of cholinergic neurotransmission by beta3-adrenoceptors results from adenosine release via equilibrative nucleoside transporters and prejunctional A1-receptor stimulation in urinary bladder. PMID: 28446460
This study showed that increased neuronal A1R expression in Rasmussen Encephalitis may be involved in prevention of seizures spread and seizures-induced damage, limitation of both seizures and inflammation atrophy in 1 cerebral hemisphere. PMID: 28789481
A1R signaling enhances A2AR-mediated neurodegeneration [review] PMID: 28441750
Mutational and computational analysis of A1-AR revealed a distinct conformation of the second extracellular loop and a wider extracellular cavity with a secondary binding pocket that can accommodate orthosteric and allosteric ligands. PMID: 28235198
second extracellular loop is a key allosteric ligand-binding determinant. PMID: 27683013
this study highlights a key role for extracellular loop 2 in A1AR orthosteric ligand binding and receptor activation. PMID: 27683014
Dysregulation in ADORA1/ADORA2A expression was associated with glioma development. PMID: 27038930
A1R mRNA levels and A1R density in PBMCs from idiopathic normal-pressure hydrocephalus patients were significantly lower than control subjects PMID: 26810542
Its central activation could be and approach to achieving a shifted homeostasis in which physiology is downregulated to a homeostatically-regulated, stable hypometabolic state. (review) PMID: 27333659
In postmortem human prefrontal cortex, adenosine A1 receptor is coupled preferentially, if not exclusively, to Galphai-3. PMID: 26213104
Results show that ADORA1 rs2228079 and ADORA2A rs5751876 polymorphisms are associated with the risk of Gilles de la Tourette Syndrome, co-morbid disorders, and may affect the age of tics onset in Polish population. PMID: 26317759
It has the intrinsic ability to form a heteromeric complex with metabotropic glutamate receptor type 1 and mutually modulate signaling. PMID: 26154847
Paeoniflorin promotes the survival of cultured cortical neurons by increasing Akt and ERK1/2 phosphorylation via A1R-mediated transactivation of EGFR. PMID: 25661317
Adenosine A1 receptor activation increases ENT1 activity via protein kinase C. PMID: 25725289
This study revealed variation of regional A1AR availability associated with ADORA1 and particularly ADORA2A SNPs, several of which had previously been shown to be associated with increased anxiety and panic disorder. PMID: 24943643
Stimulation of A1AR and A2BAR had a prominent anti-proliferative/pro-apoptotic effect on the glioblastoma stem cells. PMID: 25429616
Results document that endogenously formed adenosine can chaperone its cognate A1 receptor. PMID: 25354767
Results suggest that the anti-cancer effect of Omega-3 polyunsaturated fatty acids (Omega-3 PUFAs) on gastric cancer is at least partly dependent on activating the ADORA1-mediated apoptosis pathway. PMID: 24896321
regulation of GABA uptake occurs via modulation of the GABA transporters by the adenosine A1 (A1R) and A2A (A2AR) receptors PMID: 23657626
Induction of renal proximal tubular IL-11 is a critical intermediary in A1 adenosine receptor-mediated renal protection against acute ischemic kidney injury. PMID: 23813214
Adenosine regulates bone metabolism via A1, A2A, and A2B receptors. PMID: 23682121
Imiquimod suppresses propagation of herpes simplex virus 1 by upregulation of cystatin A via the adenosine receptor A1 pathway PMID: 22787201
A1/A2 adenosine receptor activation does not directly contribute to cutaneous active vasodilatation. PMID: 22356216
A(1) and A(2B)ARs could be potential biomarkers able to provide an early indication of SA risk and their stimulation may turn out to improve fetoplacental perfusion by increasing vascular endothelial growth factor (VEGF) and nitric oxide (NO). PMID: 22867902
In patients with stable angina ADORA1 agonist capadenoson lowers exercise heart at comparable maximum workload, which is associated with improved total exercise time and prolongation of time to ischaemia. PMID: 22370739
Results indicate a role for adenosine receptor A1 and A2A gene polymorphisms in susceptibility to apnoea of prematurity and bronchopulmonary dysplasia and in interindividual variability to caffeine response. PMID: 22462821
Data suggest that expression of ADORA1 is too low to play a major role in regulation of lipogenic gene expression in hepatocytes; studies include investigation of gene expression under influence of ADORA1 agonists. PMID: 22449383
data show that expression of A1Rs induced adipocyte differentiation, whereas A2bR expression inhibited adipogenesis and stimulated an osteoblastic phenotype PMID: 21730968
AMP and a non-hydrolyzable AMP analog (deoxyadenosine 5'-monophosphonate, ACP) directly activated the adenosine A(1) receptor PMID: 22215671
Inhibition of human mast cell activation would be a mechanism for A AR antagonists, but not A(2B) AR antagonists. PMID: 21506953
Adenosine receptor expression and activation during the differentiation of mesenchymal stem cell to osteoblasts and adipocytes, was investigated. PMID: 21590734
two genes involved in cardiovascular diseases, ADORA1 and PTGDS, were differentially up-regulated in epicardial adipose tissue compared to mediastinal and subcutaneous adipose tissue PMID: 21603615
Data suggest that adenosine deficiency may be relevant to human epilepsy and that a ketogenic diet can reduce seizures by increasing adenosine A1 receptor-mediated inhibition. PMID: 21701065
ADORA1 was expressed in nucleus, perinucleus and cytoplasm of retinal pigment epithelium. PMID: 21542986
report implicating genetic variability in the A1AR with post-traumatic seizures. PMID: 20609566
LUF5834 represents a novel high affinity radioligand for the A(1)R and may prove a useful tool to provide estimates of inverse agonist efficacy at this receptor. PMID: 20599769
results suggest that adenosine-mediated signaling in the heart requires a balance between A(1)- and A(2A)-receptors PMID: 20354569
Report fluorescence-based techniques, and in particular the development of fluorescent ligands, so study pharmacology of the adenosine A1 receptor. PMID: 20105183
Findings suggest that the adenosine A1 (and possibly coordinated with A2a) receptors contribute to dendritic cell differentiation and survival. PMID: 20085598
Studies indicate that adenosine mediates its actions by means of activation of specifiic G protein-coupled receptors, for which 4 subbtypes: A1R, A2AR, A2BR and A3R have been identified so far. PMID: 19883624
Adenosine, thus, suppresses CW2 human colonic cancer growth by inducing apoptosis as mediated via A(1) adenosine receptors. PMID: 19822392
Data suggest that adenosine receptor modulation may be useful for refining the use of chemotherapeutic drugs to treat human cancer more effectively. PMID: 19794965
ADORA1 polymorphisms may represent good candidate markers for schizophrenia research and ADORA1 may be involved in the pathophysiological mechanisms of schizophrenia in Japanese populations PMID: 19820430
Human A1 adenosine receptor overexpressed in adipose tissue of transgenic mice protects them from obesity-related insulin resistance. PMID: 11703426

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Subcellular Location

Cell membrane; Multi-pass membrane protein.

Protein Families

G-protein coupled receptor 1 family

Database Links

HGNC: 262

OMIM: 102775

KEGG: hsa:134

STRING: 9606.ENSP00000308549

UniGene: Hs.77867

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Code	CSB-RA224168A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: A549 whole cell lysate, 293 whole cell lysate, U87 whole cell lysate All lanes: ADORA1 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 37, 14 kDa Observed band size: 37 kDa IHC image of CSB-RA224168A0HU diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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ADORA1 Recombinant Monoclonal Antibody

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