ADRBK2 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA438282A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: THP-1 whole cell lysate(30µg), HEK293 whole cell lysate(30µg), A2780 whole cell lysate(30µg) All lanes: GRK3 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 80 kDa Observed band size: 80 kDa Exposure time：3min Overlay Peak curve showing SH-SY5Y cells stained with CSB-RA438282A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Have Questions?	Leave a Message or Start an on-line Chat

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Uniprot No.

P35626

Target Names

ADRBK2

Alternative Names

ADRBK2 antibody; Adrenergic; beta; receptor kinase 2 antibody; ARBK2_HUMAN antibody; BARK2 antibody; Beta adrenergic receptor kinase 2 antibody; Beta ARK 2 antibody; Beta-adrenergic receptor kinase 2 antibody; Beta-ARK-2 antibody; EC 2.7.11.15 antibody; G protein coupled receptor kinase 3 antibody; G-protein-coupled receptor kinase 3 antibody; GRK3 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human ADRBK2 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

21C6

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

ADRBK2, also known as G-protein-coupled receptor kinase 3 (GRK3), plays a central role in regulating signal transduction by phosphorylating and desensitizing activated G-protein-coupled receptors. This kinase is particularly important in modulating beta-adrenergic receptor signaling pathways, making it a key target for researchers investigating receptor regulation, cellular signaling dynamics, and related physiological processes.

This recombinant monoclonal antibody offers the consistency and reliability that demanding experimental workflows require. Produced using recombinant technology with a defined sequence, it eliminates the lot-to-lot variability that can compromise longitudinal studies or multi-site collaborations. The rabbit IgG format, generated from clone 21C6, has been affinity-purified to ensure high specificity against human ADRBK2.

Validation data demonstrates robust performance across multiple applications. In western blot analysis, the antibody detects a clear band at the predicted 80 kDa molecular weight across diverse human cell lines, including THP-1 monocytes, HEK293 cells, and A2780 ovarian carcinoma cells, confirming reliable detection across different cellular contexts. The recommended dilution range of 1:500 to 1:5000 provides flexibility for optimization in your specific experimental system. Flow cytometry validation using SH-SY5Y neuroblastoma cells shows distinct positive staining compared to isotype controls, demonstrating suitability for intracellular protein detection in fixed and permeabilized cells at dilutions between 1:50 and 1:200.

Whether you are investigating GPCR desensitization mechanisms, mapping signal transduction networks, or exploring receptor kinase biology in disease models, this antibody provides a dependable tool with demonstrated performance in both protein quantification and single-cell analysis applications.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

■ Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function

Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors.

Gene References into Functions

Study found that GRK3 was significantly overexpressed in 162 pairs of colon cancer tissues than in the matched noncancerous mucosa. These data show that aberrant expression of GRK3 plays an important role in promoting colon cancer progression through enhanced proliferation and reduced apoptosis. PMID: 29445249
hat expression of GRK3 was down-regulated in pancreatic ductal adenocarcinoma and was an independent prognostic factor PMID: 29254792
GRK3 is a new critical activator of neuroendocrine phenotypes and mediator of CREB activation in promoting neuroendocrine differentiation of prostate cancer cells. PMID: 27191986
effects of GRK3 modulation appear to be specific to chemokine-mediated migration behaviors without influencing tumor cell proliferation or survival PMID: 27049755
data are consistent with the possibility that oncogenes can induce cellular stiffness via an HDAC6-induced reorganization of the vimentin intermediate filament network PMID: 24434559
In oral squamous cell carcinomas, malignant cells and surrounding tissue overexpress the ADRBK2 gene. PMID: 21916780
A reduced cortical concentration of GRK3 in schizophrenia (resembling that in aging) may result in altered G protein-dependent signaling, thus contributing to prefrontal deficits in schizophrenia. PMID: 21784156
GRK3 is a negative regulator of cell growth whose expression is preferentially reduced in glioblastoma of the classical subtype as a consequence of activity in primary gliomagenic pathways. PMID: 22086906
mRNA levels for GRK3 were inversely correlated with systolic and diastolic blood pressure (day, night and 24 h), which suggests a protective role for GRK3 in the regulation of human blood pressure PMID: 20216086
we found no evidence of altered levels of acetylated histone H3 at the affected allele compared to the common allele PMID: 19766236
dysregulation in GRK3 expression alters signaling desensitization, and thereby predisposes to the development of bipolar disorder PMID: 12808434
the CRH-R1alpha carboxyl tail is important for regulation of receptor activity by G protein-coupled receptor kinase PMID: 15498832
ATP stimulation leads to GRK3 phosphorylation of P2X(7) receptor. PMID: 15728711
No support for a major role for GRK3 gene promoter variants in cocaine addiction. PMID: 17621168
Dysregulation in GRK3 expression alters signaling desensitization and thereby predisposes to the development of bipolar disease. PMID: 18075471
role for GRK3 in regulating CXCR4 attenuation and have provided a mechanistic link between the GRK3 pathway and the CXCR4-related WHIM(WT) disorder. PMID: 18274673
The G-384A variant may alter binding of Sp1/Sp4 transcription factors resulting in an increase in gene transcription and an increase in vulnerability to bipolar disorder. PMID: 18359007

Hide All

Protein Families

Protein kinase superfamily, AGC Ser/Thr protein kinase family, GPRK subfamily

Database Links

HGNC: 290

OMIM: 109636

KEGG: hsa:157

STRING: 9606.ENSP00000317578

UniGene: Hs.657494

Promotions

Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Chemokine signaling pathway Hedgehog signaling pathway Endocytosis Glutamatergic synapse

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

Join us with

Subscribe newsletter

Leave a message

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：
Please enter the characters shown. Click the image to refresh if unclear.

Place an order now

I. Product details

Product name *

Code *

Size *

Quantity *

Message

II. Contact details

Name *

Email address *

Company/Organization

Phone

III. Ship To

Address *

City *

State/Province *

Zip/Post code *

Country *

Attn Name *

Phone# *

IV. Bill To Same as Shipping Address