Code | CSB-RA951649A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:5000 |
IHC | 1:50-1:200 |
CUSABIO initiated an immune response by immunizing an animal with a human APC-derived peptide. B cells were then isolated from the immunized animal and fused with myeloma cells, resulting in the formation of hybridoma cells. Through rigorous screening, a single hybridoma cell clone that produces APC-specific antibodies was meticulously chosen. The selected hybridoma cells underwent RNA extraction, followed by amplification of the APC antibody-encoding genes using reverse transcription PCR. These amplified genes were subsequently cloned into an expression vector and introduced into a suitable host system for efficient antibody expression. The resulting APC recombinant monoclonal antibodies were purified from the cell culture supernatant using affinity chromatography. To ensure their quality, the binding specificity and affinity of the recombinant monoclonal APC antibody were extensively validated using three techniques including ELISA, WB, and IHC. Importantly, this antibody specifically recognizes both human and rat APC proteins.
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