ARNT Antibody

Datasheet
Code CSB-RA002121A0HU
Size US$350
Image
  • Western Blot
    Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, K562 whole cell lysate
    All lanes: ARNT antibody at 1.83μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 87, 85, 86 KDa
    Observed band size: 87 KDa

  • IHC image of CSB-RA002121A0HU diluted at 1:183 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunoprecipitating HIF-1 beta in Hela whole cell lysate
    Lane 1: Rabbit control IgG instead of CSB-RA002121A0HU in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
    Lane 2: CSB-RA002121A0HU (3μg) + Hela whole cell lysate (500μg)
    Lane 3: Hela whole cell lysate (20μg)

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Product Details

Uniprot No. P27540
Target Names ARNT
Alternative Names arnT antibody; ARNT protein antibody; ARNT_HUMAN antibody; Aryl hydrocarbon receptor nuclear translocator antibody; bHLHe2 antibody; Class E basic helix-loop-helix protein 2 antibody; Dioxin receptor antibody; Dioxin receptor nuclear translocator antibody; Drnt antibody; HIF 1 beta antibody; HIF 1beta antibody; HIF-1-beta antibody; HIF1-beta antibody; HIF1B antibody; HIF1beta antibody; Hypoxia Inducible Factor 1 antibody; Hypoxia inducible factor 1 beta antibody; Hypoxia-inducible factor 1-beta antibody; nuclear translocator antibody; Tango antibody
Species Reactivity Human
Immunogen A synthesized peptide derived from human ARNT
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Monoclonal
Isotype Rabbit IgG
Purification Method Affinity-chromatography
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form Liquid
Tested Applications ELISA, WB, IHC, IP
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:50-1:200
IP 1:200-1:1000
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunoprecipitation (IP) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Data

Function Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia.
Gene References into Functions
  1. Genetic variation at the 8q24.21 renal cancer susceptibility locus affects HIF1A and HIF1B binding to a MYC enhancer. PMID: 27774982
  2. in transcriptionally active heterodimer with AHR, which recognizes the dioxin response element (DRE) in the promoter of downstream genes, right next to the DRE-docking sites is the extensive dimerization surface that is more hydrophobic than other surface areas, indicating that the dimerization interface of the AHR transcription complex is largely dictated by hydrophobic contacts PMID: 28396409
  3. Here the authors examined the crystal structures of multi-domain NPAS1-ARNT and NPAS3-ARNT-DNA complexes, discovering each to contain four putative ligand-binding pockets. PMID: 27782878
  4. Findings reveal that certain blood cancers rely on ARNT isoform 1 to potentiate proliferation by antagonizing RelB and p53-dependent cell cycle arrest and apoptosis. PMID: 26909609
  5. The structure clearly disclosed that AhRR competitively represses AhR binding to ARNT and target DNA and further suggested the existence of an AhRR-ARNT-specific repression mechanism. This study provides a structural basis for understanding the mechanism by which AhRR represses AhR-mediated gene transcription. PMID: 28904176
  6. Study showed that polymorphism rs2228099 of the ARNT gene could be a novel susceptibility gene to essential hypertension. PMID: 27977510
  7. The results revealed a HIF-1alpha-dependent mechanism leading to ARNT upregulation in hypoxia. PMID: 27362802
  8. The protein levels of phosphorylated (p)Akt, Erk1/2, pErk1/2, HIF1alpha and HIF1beta were significantly increased by 5.89, 0.5, 0.59, 1.46 and 0.92fold, respectively, in the patients with PAH, compared with those in the controls group PMID: 27667582
  9. this study shows that ARNT is upregulated in skin from atopic dermatitis patients in China PMID: 27129092
  10. Authors report here that not only HIF1alpha but also ARNT regulates VEGF expression in 3D cancer spheroids. Results suggest the utility of the in vitro 3D cancer spheroid model for investigating angiogenesis in cancerous tissues. PMID: 27542820
  11. The results of this study indicate that ARNT depletion renders tumour cells susceptible to radiation whereas overexpression of this transcription factor confers radioresistance PMID: 26572229
  12. HIF-1beta might act as a novel cross-link between the HIF and NF-kappaB pathways in suppression of angiogenesis by LDL, while proteasome inhibitors might promote angiogenesis by reactivating this signaling cascade under hyperlipidemia. PMID: 26388611
  13. ARNT Val189Val polymorphism is not associated with endometriosis in Asians. PMID: 26101050
  14. HIF1 regulates claudin-1 in intestinal epithelium. This regulation is important for intestinal epithelial tight junction integrity. PMID: 25904334
  15. Down-regulation of ARNT promotes colorectal cancer metastasis by activating the fibronectin/integrin beta1/FAK axis. PMID: 25839165
  16. AhR/ARNT activation and overexpression of BCL6 are collectively responsible for differential expression of more than 100 genes in diffuse large B-cell lymphoma cell line. PMID: 25769544
  17. Data indicate that silencing of hypoxia-inducible factor-1 beta (HIF-1beta) sensitizes tumor cells to hypoxic apoptosis. PMID: 25068796
  18. ARNT expression in the placental vasculature mediates key angiogenic expression and fetoplacental endothalial cell angiogenesis, and low ARNT expression in FGRadv ECs appears to be a key factor in deficient angiogenesis. PMID: 25343232
  19. findings suggest that TACC3 could be recruited as a bridge to cooperatively mediate between the HIF-2alpha PAS-B.ARNT PAS-B complex, thereby participating more directly in HIF-dependent gene transcription than previously anticipated PMID: 25627682
  20. The objective of this review is therefore to highlight and summarize current knowledge regarding the hypoxia-dependent upregulation of ARNT PMID: 24849811
  21. Arnt suppressed CXCL8, but did not prevent the p65-activation directly. PMID: 25201625
  22. knockdown of ARNT in cancer cells reduced the proliferation rate and the transformation ability of those cells. PMID: 24921657
  23. Myeloid ARNT is important for innate immune function and wound healing. PMID: 24990649
  24. We demonstrate that Arnt is an interaction partner for Miz-1, and that Arnt has a functional role in the regulation of CDKN2B PMID: 24618291
  25. Here we discuss recent findings from our lab and others that define roles of the HIF-VEGF axis in the retina. PMID: 24664708
  26. ARNT gene deletion is associated with chronic lymphocytic leukemia. PMID: 24249660
  27. These findings highlight the importance of the hypoxia-sensing pathway and HIFs in clinical hematology. PMID: 24371328
  28. Understanding the DNA binding-independent functions of ARNT may identify novel therapeutic options for the treatment of a large spectrum of disease states. PMID: 23116263
  29. ARNT may modulate reactive oxygen species signaling and drug response in acute myeloid leukemia. PMID: 24220583
  30. ARNT was found to be responsible for establishing the drug-resistant properties of cancer cells by upregulation of MDR1. PMID: 23907215
  31. results suggest that polymorphism located in the promoter of the AHR gene is associated with a protective effect on vitiligo in Han Chinese populations PMID: 22211302
  32. In loco synthesis of these highly potent AhR inducers by Malassezia yeasts could have a significant impact on skin homeostatic mechanisms and disease development. PMID: 23448877
  33. results show that ER-beta suppresses HIF-1alpha-mediated transcription via aryl hydrocarbon receptor nuclear translocator (ARNT) downregulation, which may account for the tumour suppressive function of ER-beta PMID: 21435239
  34. Among a panel of five different cell lines, in 518A2 cells exposed to hypoxia-mimetic cobalt chloride, HIF-1beta is rapidly elevated on protein level. PMID: 23541582
  35. ARNT controls AREG expression and the downstream EGFR-ERK pathway in keratinocytes, at least in part, by modulating HDAC activity. PMID: 22505606
  36. Binding of the ERalpha and ARNT1 AF2 domains to exon 21 of the SRC1 isoform SRC1e is essential for estrogen- and dioxin-transcription. PMID: 22328528
  37. Ainp1 reduces the interaction between ARNT and HIF-1alpha, suppresses the formation of the HIF-1 gel shift complex, and suppresses the ARNT recruitment to the vegf promoter. PMID: 22306343
  38. miR-24 negatively regulates ARNT expression in human liver, affecting the expression of its downstream genes. miR-24 would be one of the factors underlying the mechanisms by which ARNT protein is decreased by reactive oxygen species PMID: 22387692
  39. Data show that ARNT is an important regulator of hepatocellular carcinoma (HCC) growth and metastasis and could be a promising prognostic candidate in HCC patients. PMID: 21544813
  40. The presence of five unique SNPs at the ARNT locus are shown. PMID: 21828933
  41. ARNT may regulate many processes relevant to the biology of age related macular degeneration. PMID: 22183315
  42. mutations on ARNT PAS-B modulate coactivator selectivity and target gene induction by HIF in vivo, demonstrating a bifunctional role for transcriptional regulation by PAS domains within bHLH-PAS transcription factors PMID: 21512126
  43. NF-kB controls the levels of HIF-1a and HIF-1b genes by direct regulation. PMID: 21298084
  44. Cloning and functional analysis of the HIF-1beta promoter identifies a prominent region for interferon (IFN)-gamma-dependent repression. PMID: 21199896
  45. These results reveal a novel mechanism by which ARNT acts as a modulator to bridge the c-Jun/Sp1 interaction and plays a role in EGF-mediated gene expression under normoxic conditions. PMID: 20508969
  46. Reverse transcriptase-polymerase chain reaction analysis disclosed the existence of a ETV6-ARNT fusion gene in a patient with childhood T lymphoblastic leukemia. PMID: 20804916
  47. the expression of miR-101 is also modulated at different physiological conditions, such as androgen stimulation and HIF-1alpha/HIF-1beta induction. PMID: 20478051
  48. the expression of ARNT protein is significantly reduced in aryl hydrocarbon receptor interacting protein mutation+ tumors PMID: 19850893
  49. SRC-1, NCoA-2, and p/CIP are capable of independently enhancing TCDD-dependent induction of a luciferase reporter gene by the AHR/ARNT dimer PMID: 12024042
  50. modification by SUMO-1 chiefly at Lys(245) within the PAS domain of this protein, both in vivo and in vitro PMID: 12354770
  51. function as potent coactivator of estrogen receptor-dependent transcription PMID: 12754377
  52. formation of stable protein-DNA complexes by DR/Arnt and HIF-1alpha/Arnt heterodimers with their cognate DNA sequences requires Per/Arnt/Sim A domains PMID: 14638687
  53. nucleotide preference of the heterodimers of HLF and Arnt, and of AHR and Arnt PMID: 15190133
  54. Studies using a small interfering RNA to down-regulate Arnt protein expression revealed that TCDD-induced G(1) arrest is absolutely dependent on the Arnt protein. PMID: 15492120
  55. Stat3 is required for both basal and growth signal-induced expression of HIF-1 PMID: 16007214
  56. The role of ARNT/HIF1beta and altered gene expression in impaired beta cell function and the pathogenesis of human type 2 diabetes. PMID: 16096055
  57. Ainp2 enhances the 3-methylchloranthrene-induced activity in HepG2 cells, suggesting that Ainp2 plays a role in the Arnt-dependent function. PMID: 16111650
  58. Phosphorylation of Ser77 within the alternative exon of ARNT has a major influence on the activity of alternatively (Alt) spliced ARNT homodimers, but not an Alt ARNT heterodimer. PMID: 16129408
  59. In our cohort of patients, the polymorphism in codon 511 of the ARNT gene is not associated with RM. PMID: 16364012
  60. dioxin receptor is silenced by promoter hypermethylation in human acute lymphoblastic leukemia through inhibition of Sp1 binding PMID: 16410262
  61. for the bHLH.PAS transcription factors Dioxin Receptor and Arnt, the DR PAS A domain has a role in dimerization and affinity for an atypical E-box DNA sequence PMID: 16520375
  62. In hypoxia, HIF-alpha is stabilized and either dimerizes with HIF-beta to form transcriptionally active HIF for a hypoxia response, or it interacts with unrelated proteins, enabling convergence of HIF oxygen sensing with other signaling pathways. PMID: 16554418
  63. xenobiotic (TCDD) treatments of breast cancer cells containing reduced levels of BRCA1 cause the transcription factor ARNT to become unstable PMID: 16567799
  64. Overexpression of presenilin-1 increased HIF-1beta, suggesting that HIF is downstream of presenilin PMID: 18174159
  65. ARNT variants are unlikely to explain the linkage signal on chromosome 1q, but may alter insulin secretion in nondiabetic subjects PMID: 18366646
  66. identified hypoxia inducible factor 1beta (HIF1beta) as a TG2 binding partner PMID: 18375543
  67. The downregulation of ETS1 expression with small interfering RNA (siRNA) involves HIF1beta in regulating hypoxia-inducible genes. PMID: 18381358
  68. reduced availability of glucose under hypoxia downregulates HIF-1 in part through the inhibition of HIF-1 alpha mRNA PMID: 18762723
  69. Hybrids of the bHLH and bZIP protein motifs display different DNA-binding activities in vivo vs. in vitro. PMID: 18949049
  70. subtypes of VHL mutations support an intermediate level of HIF-1alpha and HIF-2alpha regulation via a remnant VBC complex. PMID: 19030229
  71. crystal structures of the heterodimer formed by the C-terminal PAS domains from the HIF2alpha and ARNT subunits of the HIF2 transcription factor, both in the absence and presence of an artificial ligand. PMID: 19129502
  72. identification of ARNT as a CD30-interacting protein that modulated the activity of the RelB subunit of the transcription factor NF-kappaB; findings indicate that ARNT functions in concert with RelB in a CD30-induced negative feedback mechanism PMID: 19131627
  73. ARNT plays an important role in EGF-regulated COX-2 gene expression and may thus be related to either a cause or a consequence of tumorigenesis in cervical cancer PMID: 19203995
  74. The SUMOylation of both AhRR and Arnt is important for the efficient transcriptional repression activity of the AhRR/Arnt heterodimer PMID: 19251700
  75. The results of the present study demonstrate that HIF-1alpha and HIF-1beta enhances expression of VEGF and glucose metabolism-related genes in response to hypoxia in gastric cancer. PMID: 19287200
  76. A deficiency of ARNT action in the liver could contribute to the altered metabolic function in humans with type 2 diabetes. PMID: 19416713

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Subcellular Location Nucleus
Database Links

HGNC: 700

OMIM: 126110

KEGG: hsa:405

STRING: 9606.ENSP00000351407

UniGene: Hs.632446

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