Code | CSB-RA002121A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:5000 |
IHC | 1:50-1:200 |
IP | 1:200-1:1000 |
The generation of the ARNT recombinant monoclonal antibody relies on the utilization of DNA recombinant technology and in vitro genetic manipulation. The process begins with immunizing animals using a synthesized peptide derived from human ARNT, followed by the isolation and selection of positive B cells. These positive B cells undergo further screening and identification of individual clones. The light and heavy chains of the ARNT antibody are then amplified through PCR and inserted into a plasmid vector. This recombinant vector is introduced into a host cell line to facilitate antibody expression. The ARNT recombinant monoclonal antibody is purified from the cell culture supernatant using affinity chromatography. It exhibits specific binding to human ARNT protein and is well-suited for four applications, including ELISA, WB, IHC, and IP.
The ARNT protein is involved in the regulation of gene expression. It forms a heterodimer with other transcription factors, such as the AhR, HIF-1, and Nrf2, which allows them to bind to DNA and activate or repress the transcription of specific genes. ARNT plays an essential role in several cellular processes, including cell proliferation, differentiation, metabolism, and response to environmental stress. It also has a critical function in embryonic development, particularly in the formation of the cardiovascular and respiratory systems. ARNT has been implicated in various pathophysiological conditions, such as cancer, diabetes, and cardiovascular diseases.
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