ARPC5 Recombinant Monoclonal Antibody

ARPC5, also known as p16-ARC, serves as the smallest subunit of the Arp2/3 complex, a critical molecular machine that drives actin filament nucleation and branching. This complex plays fundamental roles in cell motility, endocytosis, and the maintenance of cellular architecture, making ARPC5 an important target for researchers investigating cytoskeletal dynamics, cancer cell migration, and neuronal development.

This recombinant monoclonal antibody, clone 4H8, offers the reproducibility and consistency that demanding experimental workflows require. Generated through recombinant technology with a defined sequence, it eliminates the lot-to-lot variability that can compromise longitudinal studies or multi-site collaborations. The rabbit host and monoclonal nature ensure high specificity for your target across repeated experiments.

Validation studies demonstrate reliable performance across multiple applications and sample types. In western blot analysis, the antibody detects a clean 16 kDa band matching the predicted molecular weight of ARPC5 in HeLa cells, U-87MG glioblastoma cells, and rat brain tissue lysate, confirming cross-species reactivity between human and rat samples. Immunofluorescence staining in HeLa cells reveals the expected cytoplasmic localization pattern consistent with ARPC5's role in actin network regulation. Flow cytometry validation further demonstrates the antibody's utility for quantitative single-cell analysis, with clear separation between specific signal and isotype control populations.

The flexibility to move between western blotting, immunofluorescence, and flow cytometry using a single validated reagent streamlines experimental design for researchers studying actin dynamics in contexts ranging from basic cell biology to disease-relevant models including cancer metastasis and neurodegenerative conditions.