BAG2 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA104772A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: Hela whole cell lysate(30µg), Jurkat whole cell lysate(30µg), PC-3 whole cell lysate(30µg) All lanes: BAG2 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 24 kDa Observed band size: 24 kDa Exposure time：1min
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Target Background

Product Details

Uniprot No.

O95816

Target Names

BAG2

Alternative Names

BAG family molecular chaperone regulator 2 (BAG-2) (Bcl-2-associated athanogene 2), BAG2

Species Reactivity

Human

Immunogen

A synthesized peptide from human BAG2 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

11B5

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

BAG2 serves as a critical co-chaperone that regulates protein quality control pathways, working alongside HSP70 family members to influence protein folding, degradation, and cellular stress responses. This molecular chaperone regulator has gained significant research attention for its roles in proteasomal degradation, tau protein homeostasis relevant to neurodegenerative disease, and emerging connections to cancer biology where it may influence tumor suppressor pathways.

This recombinant monoclonal antibody, clone 11B5, offers the reproducibility essential for longitudinal studies and multi-site collaborations. Because the antibody sequence is defined and production occurs in controlled recombinant systems, you can expect consistent performance across different lots—eliminating the variability that can complicate data interpretation when working with traditional hybridoma-derived antibodies.

Validation by western blot demonstrates reliable detection of BAG2 across multiple human cell lines, including HeLa, Jurkat, and PC-3 lysates. The antibody produces a clean signal at the expected 24 kDa molecular weight, confirming specific recognition of the target protein without apparent degradation products or non-specific bands under standard conditions. This performance across epithelial, T-lymphocyte, and prostate carcinoma cell backgrounds suggests robust utility for researchers working with diverse experimental models. The validated dilution range of 1:500 to 1:2000 for western blotting provides flexibility to optimize signal intensity based on your specific sample conditions and detection systems.

Whether investigating chaperone-mediated autophagy, exploring BAG2's influence on protein aggregation disorders, or examining its potential contributions to oncogenic signaling, this antibody provides a dependable tool for characterizing BAG2 expression and regulation in human experimental systems.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Co-chaperone for HSP70 and HSC70 chaperone proteins. Acts as a nucleotide-exchange factor (NEF) promoting the release of ADP from the HSP70 and HSC70 proteins thereby triggering client/substrate protein release.

Gene References into Functions

the nucleotide exchange factor (NEF) Bag2 stimulates SV40 release from Hsc70, thereby enabling successful virus arrival at the cytosol, which leads to infection. Hsp105, another NEF of Hsc70, displays a function overlapping that of Bag2, underscoring the importance of this release reaction. PMID: 29769335
A comprehensive review of the structure, functions, and protein interactions of BAG2 PMID: 28536620
Data show that BAG2 Inhibits CHIP-Mediated HSP72 ubiquitination in aged cells. PMID: 28042827
repression of BAG2 expression or BAG2 activity by cold-sensitive pathways, as modeled in undifferentiated and differentiated cells, respectively, may be a causal factor in the accumulation of cytotoxic hyperphosphorylated tau protein. PMID: 26208804
NF-kappaB-mediated modulation of BAG2 expression regulates the shift between the neurotrophic and neurotoxic effects of Abeta1-42. PMID: 25985852
Thus, BAG2 promotes mutant p53 accumulation and gain-of-function in tumor growth, metastasis and chemoresistance. PMID: 26271008
This study showed that BAG2 (Bcl-2 associated athanogene family protein 2) and BAG5 (Bcl-2-associated athanogene family protein 5) stabilise pathogenic ataxin3-80Q by inhibiting its ubiquitination PMID: 25006867
this data correlate BAG2 to PINK1 for the first time, strengthening the important role of BAG2 in Parkinson disease -related neurodegeneration. PMID: 24383081
BAG2 binds to the carboxyl terminus of Hsp70-interacting protein (CHIP) and provides a cochaperone-dependent regulatory mechanism for preventing unregulated ubiquitylation of misfolded proteins by CHIP PMID: 16169850
The activity of BAG-2 resembles that of the previously characterized Hsc70 cochaperone and CHIP inhibitor HspBP1. PMID: 16207813

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Database Links

HGNC: 938

OMIM: 603882

KEGG: hsa:9532

STRING: 9606.ENSP00000359727

UniGene: Hs.745046

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Protein processing in endoplasmic reticulum Target Therapy

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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