Product Details

Uniprot No.

Q16611

Target Names

BAK1

Alternative Names

BAK1; BAK; BCL2L7; CDN1; Bcl-2 homologous antagonist/killer; Apoptosis regulator BAK; Bcl-2-like protein 7; Bcl2-L-7

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human BAK1

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

8D1

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, FC, IP

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
IP	1:200-1:1000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol
Immunoprecipitation (IP) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

BAK1, also known as Bcl-2 homologous antagonist/killer, serves as a critical pro-apoptotic regulator within the intrinsic mitochondrial death pathway. As a member of the Bcl-2 protein family, BAK1 functions by permeabilizing the outer mitochondrial membrane upon activation, enabling cytochrome c release and subsequent caspase cascade initiation. Understanding BAK1 expression and regulation is essential for researchers investigating apoptotic mechanisms, cancer biology, and therapeutic strategies targeting programmed cell death.

This recombinant monoclonal antibody, generated against a synthetic peptide derived from human BAK1, offers the consistency and reliability that demanding experimental workflows require. As a sequence-defined recombinant clone, it eliminates the lot-to-lot variability inherent to traditional hybridoma-derived antibodies, ensuring reproducible results across long-term studies and multi-site collaborations.

Validation across multiple applications demonstrates this antibody's experimental flexibility. Western blot analysis confirms specific detection of BAK1 at the predicted 24 kDa molecular weight across diverse human cell lines including MCF7, THP-1, HEK293, A431, and HepG2, with effective dilutions ranging from 1:500 to 1:5000. Immunohistochemical staining has been successfully performed in paraffin-embedded human endometrial tissue using citrate buffer antigen retrieval, while immunoprecipitation studies in HEK293 lysates confirm the antibody's capacity for protein enrichment applications. Flow cytometry validation in HeLa cells further extends its utility for single-cell analysis of intracellular BAK1 levels.

Whether investigating apoptotic signaling in cancer models, characterizing cell death pathways, or examining BAK1 expression patterns across tissues, this antibody provides researchers with a dependable tool for advancing cell biology research.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Code	CSB-RA624111A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: MCF7 whole cell lysate(30µg), THP-1 whole cell lysate(30µg), 293 whole cell lysate(30µg), A431 whole cell lysate(30µg), HepG2 whole cell lysate(30µg) All lanes: BAK1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 24 kDa Observed band size: 24 kDa Exposure time: 60s IHC image of CSB-RA624111A0HU diluted at 1:50 and staining in paraffin-embedded human endometrial tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody Immunoprecipitating BAK1 in HEK293 whole cell lysate Lane 1: Rabbit control IgG instead of CSB-RA624111A0HU in HEK293 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-RA624111A0HU (3μg) + HEK293 whole cell lysate (500μg) Lane 3: HEK293 whole cell lysate (20μg) Overlay histogram showing Hela cells stained with CSB-RA624111A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4℃. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4℃. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
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BAK1 Recombinant Monoclonal Antibody

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